简介:AIM:Toinvestigatetheantifibroticeffectofthefreeze-driedbilayeredfibrin-bindingamnioticmembraneasadrugdeliverysystemonglaucomasurgeryinrabbitmodel.Theaimofthisstudywastoprepareanovellocaldeliverysystemforthesustainedandcontrollablereleaseof5-Fu.METHODS:Twenty-fourJapanesewhiterabbitswererandomizedintothreegroups:theexperimentalgroup(oculartrabeculectomyincombinationwith5-Fuloadedfreeze-driedbilayeredfibrin-bindingamnioticmembranetransplantation),thecontrolgroup(oculartrabeculectomyincombinationwith5-Fu)andtheblankgroup(singletrabeculectomy).HEstaining,massionstainingandimmunohistochemistryforα-SMAwereperformedondays7,14,21and30followingsurgery.Theconcentrationof5-Fuinrabbitaqueoushumorwasexaminedbyhighperformanceliquidchromatography(HPLC)3daysafterthesurgery.RESULTS:Statisticaldifferenceswerenotedinintraocularpressureamonggroupsonday7,14,21and30followingsurgery.Histologyfurtherdemonstratedthattrabeculectomyincombinationwithfreeze-driedbilayeredfibrin-bindingamnioticmembraneyieldedwellwoundhealingandnoscarformationandwasbeneficialforlongtermeffect.CONCLUSION:HPLCshowedagoodslow-releaseeffectwithfreeze-driedbilayeredfibrin-bindingamnioticmembrane.
简介:目的调查上海市奉贤区奉城镇60岁以上老年人群干眼的患病率及相关影响因素。方法2013年6~12月对上海市奉贤区奉城镇60岁以上人群采用随机整群分组抽样法,进行问卷调查,对有阳性症状的受检者做裸眼视力、裂隙灯、基础泪液分泌试验(SⅠt)、泪膜破裂时间(BUT)、角膜荧光素染色(FL)、睑板腺功能检查。结果随机抽取受检者2387例,实际受检者2058例,应答率为86.2%。受检者中干眼患者295例,患病率为14.3%,女性患病率为18.0%(208/1153),男性患病率为9.6%(87/905),女性高于男性(χ^2=29.22,P〈0.05);80岁以上人群的干眼患病率为21.3%,70~79岁为15.8%,60~69岁为9.2%(χ^2=36.61,P〈0.005)。结论上海市奉贤区奉城镇60岁以上人群中,女性的干眼患病率高于男性。干眼患病率随年龄增长而升高,眼干涩、视疲劳、异物感为最常见症状,局部及全身多种因素均影响干眼的发生。干眼的社区防治对郊区眼病防治工作有着重要的意义。
简介:目的总结45例60岁以上老年患者行乙状窦后进路桥小脑角手术的围手术期治疗经验.方法对我科1991年1月至2000年1月行该手术治疗的45例老年患者进行回顾性分析.其中三叉神经痛37例;舌咽神经痛3例;半面痉挛4例;桥小脑角胆脂瘤1例.术前检查异常的有:心电图12例,占26.67%;胸部X线6例,占13.3%;颅脑CT2例,占4.44%,高血压病9例,占20%;糖尿病5例,占11.1%;肝功能异常3例,占6.67%;白细胞低2例,占4.44%;血小板减少2例,占4.44%.结果所有患者均顺利完成手术,术后无严重并发症,均治愈出院,随访2~9年,症状无复发.结论老年患者虽然体质较弱,检查结果异常多,但只要正确处理围手术期的异常,提高手术技巧,老年患者完全能够耐受此类手术并顺利康复.
简介:AIM:Toinvestigatethepossiblerelationshipbetweentheinfluencingfactorsoccurringbeforeandduringbirthinfull-terminfantsandtheoutcomeofretinopathy.·METHODS:Totally816full-terminfantsadmittedintheneonateintensiveunitofBoaiHospitalofZhongshanbetween1May,2008and30June,2011wereincludedinthestudy.Fundusexaminationwasperformedandevaluatedindividuallyonthemattheageof48hoursafterdelivery,2weeksand1month.Somepossibleriskfactorshappeningprenatallyorduringdeliverysuchaspregnantrelatedhypertension,placentaprevia,placentalabruptionetc,aswellassomeneonatalriskfactorssuchasneonatalasphyxia,hypoxic-ischemicencephalopathy(HIE),lowbirthweightetc,wererecordedandevacuated.Thentheeffectoftheriskfactorsoffull-terminfantsonretinopathywasstudied.·RESULTS:Theincidenceofretinalhemorrhageoffull-terminfantswithprenatalpregnantrelatedhypertension(PRH)ofthemother(43.6%)wassignificantlyhigherthanthatoffull-terminfantswithout(8.0%).(P<0.001).Theincidenceofretinalhemorrhageoffull-terminfantswithneonatalasphyxiaand/orhypoxic-ischemicencephalopathy(HIE)(29.3%)wassignificantlyhigherthanthatofthosewithout(15.7%),butcorrelationwasnotfoundbetweentheseverityofretinahemorrhageandthedegreeofhypoxicdisease.Apalecolorofopticdiscwasassociatedwithalowbirthweightoffull-terminfant.Full-terminfantswithbirthweighlessthan2500ghadasignificanthigherincidenceofretinopathythanthosewithbirthweightequalormorethan2500g(P<0.001).·CONCLUSION:Themaininfluencingfactorswhichleadtoretinopathyofhighriskfull-terminfantsareprenatalfactorssuchasPRH,andsomeneonatalriskfactorssuchasasphyxia,hypoxic-ischemicencephalopathy,andlowbirthweight.
简介:Oculardrugtransportbarriersposeachallengefordrugdeliverycomprisingtheocularsurfaceepithelium,thetearfilmandinternalbarriersoftheblood-aqueousandblood-retinabarriers.Oculardrugdeliveryefficiencydependsonthebarriersandtheclearancefromthechoroidal,conjunctivalvesselsandlymphatic.Traditionaldrugadministrationreducestheclinicalefficacyespeciallyforpoorwatersolublemoleculesandfortheposteriorsegmentoftheeye.Nanoparticles(NPs)havebeendesignedtoovercomethebarriers,increasethedrugpenetrationatthetargetsiteandprolongthedruglevelsbyfewinternalsofdrugadministrationsinlowerdoseswithoutanytoxicitycomparedtotheconventionaleyedrops.Withtheaidofhighspecificityandmultifunctionality,DNANPscanberesultedinhighertransfectionefficiencyforgenetherapy.NPscouldtargetatcornea,retinaandchoroidbysurficialapplicationsandintravitrealinjection.ThisreviewisconcernedwithrecentfindingsandapplicationsofNPsdrugdeliverysystemsforthetreatmentofdifferenteyediseases.
简介:AIM:Toinvestigatethecharacteristicsandcriterionofgraftrejectioninmicemodel.METHODS:C57BL/6orBALB/cmicecornealgraftsweregraftedontoBALB/chosts.Eachgroupwasdividedintotwosubgroupsaccordingtothecornealopacityscores12daftertransplantation.Thecharacteristicsofopacityandneovascularizationwereobserved.Miceofthe12th,50thdayaftertransplantation,thegraftsbiopsyofmiceinallogeneicgroup1,whichopacityscoreexceed3,werepreparedforhistologicalobservationandthoserestoretransparentwereendothelialstained.RESULTS:Therewasnodifferenceofcornealopacityscoreonthe7thand12thdayafteroperation;thehistologicalresultshadnodisparitybetweensyngeneicgroupandallogeneicgroup.Onthe12thdayaftersurgery,theturbiditycurvewasapparentingraftswithopacityscore<2.Mononuclearcellswereshowningraftswithopacityscorereached3inallogeneicgroup1.Differentrejectionperformancewasobservedintissuesectionsonthe50thdayaftersurgery.CONCLUSION:Grafts,opacityscoreexceeds3fromthe7thtothe12thdayafteroperationcouldnotbejudgedasarejection.Weshouldpaymoreattentiontothevariationofgraftsopacitysince12daftercornealtransplantation.
简介:AIM:Todefinetheultrasonographicstructureofnormallowereyelidanatomiccompartmentsandtheirspacialrelationshipindynamicmotion.METHODS:Highresolutionultrasound(15MHz)wasperformedonthelowereyelidsof7normalsubjects.Movementsofthelowereyelidanditscompartmentswerevisualizedwithultrasound.Inaddition,themaximalexcursionareaofthelowereyelidfatcompartmentsandretractormotionswasmeasuredbeforeandaftermotion.RESULTS:Theorbicularismusclecouldbeseenasanecholucentstructurebetweenthedermisandtheechodencefatpads.Lowereyelidfatpadseemstobedividedinto2compartmentsasrangeofmotionanddirectionofmovementofeachofthemvaries.Itseemsthatthesecompartmentshavealsodifferentbehavior.Themeasuredprofileareaofthevisiblenormallowereyelidfatpadsduringmovementofglobefromup-gazetodown-gazedecreasedby50%.Orderofmovementoflowereyelidstructuresseemstobeasfollows:afterglobemovementfistweseeretractormovement,anteriororbitalfatpad,thenskinandseptum,andfinallymovementofinferiorfatpad.CONCLUSION:Ultrasoundrepresentsanoninvasivetoolforthevisualizationoflowereyelidmorphology.Expandingitsapplicationcouldhelpusunderstandthecompartmentalchangesinphysiologicaleyelidmovement,inaginganddiseasedstudypopulations,aswellasassessoperativeoutcomes.
简介:<正>DearSir,IamProf.BeuyJoobfromSanitation1MedicalAcademicCenter,Bangkok,Thailand.Iwritetodiscusstherecentpublicationonproteomicanalysisindiabeticretinopathy(DR).Liuetal[1]concludedthattheirapproachbytwodimensionalfluorescencedifferencegelelectrophoresis(2D-DIGE)combinedwithmatrix-assistedlaser
简介:AIM:Toexaminethemechanismofthedevelopmentofpseudoexfoliation(PSX)syndromeviabothcytokineformationandendothelialvasorelaxingandgrowthfactorsthatwillprovideusnewtherapeuticinsightsforthetreatment.METHODS:Thisisacrosssectionalstudyincludedtwogroups;Group1:controlpatientswithnuclearcataract(n=20,aged51-80years).Group2:PSXpatientswithnuclearcataract(n=18,aged50-90years).Patientswithotherophthalmicproblemsandsystemicdiseaseswereexcluded.Vascularendothelialgrowthfactor(VEGF),interleukin-6(IL-6)andinterleukin-1β(IL-1β)andnitrotyrosinelevelsweredeterminedthroughserumsamplesbyEnzyme-linkedimmunosorbentassay(ELISA)method.Nitrite-nitratelevelsweremeasuredwithphotometricendpointdetermination.RESULTS:Therewerenosignificantdifferencesbetweenthegroupsintermsofage,VEGF,IL-1β,nitrite-nitrateandnitrotyrosine.ThesignificantresultswerethemeanIL-6levelsthatwerehigherinPSXgroup2(37.68±29.52pg/mL)comparedtothatincontrolgroup1(15.32±10.08pg/mL)(P<0.001).CONCLUSION:SeveralinteractingandextendingbiochemicalpathwaysmayleadtothepromotionofVEGFandIL-6expressions.IL-6whichistheonlyalteredmarkerinourstudymayindirectlycauseanincreaseofvascularpermeabilityandneovascularization.WesuggestinflammationasafactorthatcanbeinvolvedinetiopathogenesisofPSX.
简介:·Descemet’smembranedetachment(DMD)canbeapotentiallyseriouscomplicationofintraocularsurgeryoroculartrauma.Thecauseisnotveryclear.WearetryingtoremindanawarenessofthespectrumofDMDresultingfromtrabeculectomybypresentingacaseofextensiveDMDaftertrabeculectomywhichwassuccessfullyrepaired.