简介:AIM:Toinvestigatethecharacteristicsandcriterionofgraftrejectioninmicemodel.METHODS:C57BL/6orBALB/cmicecornealgraftsweregraftedontoBALB/chosts.Eachgroupwasdividedintotwosubgroupsaccordingtothecornealopacityscores12daftertransplantation.Thecharacteristicsofopacityandneovascularizationwereobserved.Miceofthe12th,50thdayaftertransplantation,thegraftsbiopsyofmiceinallogeneicgroup1,whichopacityscoreexceed3,werepreparedforhistologicalobservationandthoserestoretransparentwereendothelialstained.RESULTS:Therewasnodifferenceofcornealopacityscoreonthe7thand12thdayafteroperation;thehistologicalresultshadnodisparitybetweensyngeneicgroupandallogeneicgroup.Onthe12thdayaftersurgery,theturbiditycurvewasapparentingraftswithopacityscore<2.Mononuclearcellswereshowningraftswithopacityscorereached3inallogeneicgroup1.Differentrejectionperformancewasobservedintissuesectionsonthe50thdayaftersurgery.CONCLUSION:Grafts,opacityscoreexceeds3fromthe7thtothe12thdayafteroperationcouldnotbejudgedasarejection.Weshouldpaymoreattentiontothevariationofgraftsopacitysince12daftercornealtransplantation.
简介:目的:评价图形视觉诱发电位(patternvisualevokedpotentials,P-VEP)、眼底照相、视野和光学相干断层扫描(opticalcoherencetomography,OCT)对青光眼患者检查的可靠性。方法:对83例95眼青光眼患者应用眼科电生理仪、眼底照相、视野和OCT,根据P-VEP、眼底照相、OCT、视野检查结果的阳性率进行相关性分析。结果:患者83例95眼中,视野检查成功获取阳性病例图像73眼(76.8%),无法获取图像22眼(23.2%);眼底照像成功获取阳性病例图像75眼(78.9%),无法获取图像20眼(21.1%);获得P-VEP阳性病例47眼(49.5%),未见明显异常26眼(27.3%),无法采集图像22眼(23.2%);成功获取OCT阳性病例图像81眼(85.3%),无法获取OCT图像14眼(14.7%)。结论:视觉诱发电位、眼底照相、视野和光学相干断层图像结果对青光眼患者的视功能状况进行综合评估。
简介:目的通过快速评估可避免盲系统评估四川省冕宁县50岁以上人群视力障碍的患病率和致残原因。方法在2011年6月至8月使用RAAB系统对四川省冕宁县50岁以上居民采用分层、整群随机抽样方法抽取2850人,根据人口比例,分为57个人群组,每个人群组人数为50人,每个人群组采用紧凑段抽样(compactsegmentsampling,CSS),当紧凑段抽样不可行时,采用配额抽样。使用简易视力卡检测视力,用便携式裂隙灯和直接检眼镜检查晶状体及眼底情况以明确导致视力残疾的原因。结果在冕宁县抽样调查的2850人中,2817人接受了检查(98.8%),参照1973年世界卫生组织(WorldHealthOrganization,WHO)视力损伤标准,共有视力残疾者537人,患病率为19.1%,其中盲111人,盲的患病率为3.9%(95%可信区间:3.2%-4.7%),严重视力损伤69人,其患病率2.4%(95%可信区间:1.8%-3.1%);视力损伤357人,其患病率12.7%(95%可信区间:10.5%-14.9%),其中可避免盲占87.4%。结论1.冕宁县50岁及以上人群盲的患病率为3.9%,严重视力损伤的患病率2.4%;视力损伤的患病率12.7%,其中可避免盲占87.4%。未经治疗的白内障仍然是该县最主要的致残原因。2.RAAB系统作为一种在人群中快速筛查可避免盲的调查方法,准确性好,简单实用、高效,重复性强。
简介:目的:探索一种临床实用的前房深度相对定量测量法,初步确定老年人前房深度的正常参考值。方法:裂隙灯光源外转45°,受检眼直视裂隙光,在6点时钟方向测量六个点的前房深度(ABCDEF),以角膜缘处的角膜厚度(CT值)为1计。A:角巩膜缘黑白交界处;B:A点上移1CT;C:B点上移1CT;D:虹膜最高点;E:瞳孔缘;F:晶状体前极。测量了50~75岁的正常老年人,229例229眼,采用双盲法对22例44眼进行了11次重复性检测。结果:女性组ABCDEF点的前房深度均值分别为0.38,0.58,0.89,2.10,2.65和3.26CT;男性组分别为0.71,1.02,1.43,2.37,2.90,3.41CT。女性组每个点的深度均比男性浅(P〈0.05),越近周边部越显著。结论:前房深度六点相对定量测量法可重复性好,实用性强。
简介:AIM:Anaerobicbacteriacancauseocularinfections.WetestedtheOxyPlateTMAnaerobicSystem(OXY)toisolatepertinentanaerobicbacteriathatcancauseoculardisease.METHODS:OXY,whichdoesnotrequiredirectanaerobicconditions(i.e.bags,jars),wascomparedtoconventionalisolationofincubatingculturemediainanaerobicbags.Standardcoloniescountswereperformedonanaerobicocularbacterialisolatesunderaerobicandanaerobicconditions(anaerobicbags)usingagarmedia:1)OXY(aerobiconly),2)5%sheepblood(SB),3)Chocolate,and4)Schaedler.Thebacteriatestedwerede-identifiedocularisolatesculturedfromendophthalmitisanddacryocystitisthatinclude10Propionibacteriumacnesand3Actinomycesspecies.Thecolonycountsforeachbacteriaisolate,oneachculturingcondition,wererankedfromlargesttosmallest,andnon-parametricallycomparedtodeterminethebestculturingcondition.RESULTS:Allanaerobicconditionswerepositiveforalloftheanaerobicisolates.SBandSchaedler’sagarunderaerobicconditionsdidnotsupportthegrowthofanaerobicbacteria.SparsegrowthwasnotedonchocolateagarwithPropionibacteriumacnes.Asananaerobicsystem,SBinananaerobicbagisolatedhighercolonycountsthanOXY(P=0.0028)andchocolateagar(P=0.0028).CONCLUSION:AlthoughOXYdidnottesttobemoreefficientthanotheranaerobicsystems,itappearstobeareasonablealternativeforisolatinganaerobicbacteriafromocularsites.Theuseofanagarmediuminaspeciallydesignedplate,withouttherequirementofananaerobicbag,renderedOXYasanadvantageoverotheranaerobicsystems.
简介:Thekeratoprosthesis(KPro;artificialcornea)isaspecialrefractivedevicetoreplacehumancorneabyusingheterogeneousformingmaterialsfortheimplantationintothedamagedeyesinordertoobtainacertainvision.Themainproblemsofartificialcorneaarethebiocompatibilityandstabilityofthetissueparticularlyinpenetratingkeratoplasty.Thecurrentstudiesoftissue-engineeredscaffoldmaterialsthroughcomprisingcompositesofnaturalandsyntheticbiopolymerstogetherhavedevelopedanewwaytoartificialcornea.Althoughawideagreementthatthelong-termstabilityofthesedeviceswouldbegreatlyimprovedbythepresenceofcorneacells,modificationofkeratoprosthesistosupportcorneacellsremainselusive.Mostofthestudiesoncornealsubstratematerialsandsurfacemodificationofcompositeshavetriedtoimprovethegrowthandbiocompatibilityofcorneacellswhichcannotonlyreducethestimulusofheterogeneousmaterials,butalsomoreimportantlycontinuousandstablecorneacellscanpreventthedestructionofcollagenase.Thenecrosisofstromaandspontaneousextrusionofthedevice,allowformaintenanceofaprecornealtearlayer,andplaytheroleofensuringagoodopticalsurfaceandresistingbacterialinfection.Asaresult,improvementincornealcellshasbeenthemainaimofseveralrecentinvestigations;someefforthasfocusedonbiomaterialforitswellbiologicalpropertiessuchaspromotingthegrowthofcorneacells.Thepurposeofthisreviewistosummarythegrowthstatusofthecornealcellsaftertheimplantationofseveralartificialcorneas.
简介:目的:观察糖尿病视网膜病变(diabeticretinopathy,DR)患者行全视网膜光凝(panretinalphotocoagulation,PRP)后服药前及服药2mo后患眼的全视野视网膜电图(fullfieldelectroretinogram,ERG)变化,探讨递法明片对DR暗适应功能的保护作用。方法:选择在我医院就诊的重度非增殖性糖尿病视网膜病变(nonproliferativediabeticretinopathy,NPDR)患者55例55眼,随机分为治疗组和对照组。两组均行全视网膜光凝,治疗组光凝后口服递法明片,对照组口服维生素B1片2次/d,10mg/次。PRP后服药前及服药2mo后行全视野视网膜电图检查,观察其暗视视杆反应的变化并进行统计学处理。结果:两组患者服药前及服药2mo后,暗视视杆反应bT值组内及组间比较差异均无统计学意义(P〉0.05);治疗组bA值服药2mo后与服药前相比振幅升高,差异有统计学意义(P〈0.05),与对照组相比治疗组振幅升高较大,差异有统计学意义(P〈0.05)。结论:递法明片可减轻PRP治疗对视网膜的损害,改善患者的暗适应功能。