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简介：AbstractObjective:Amino acyl modification of lysine residues is an essential mechanism of nutrient sensing that regulates various biological functions including reproduction. At present, the lack of pan-specific antibodies for a recently identified lysine valylation hinders the characterization and detection of this modification. The objective of this study is to raise pan-specific antibodies that may facilitate the identification of novel expression patterns of lysine valylation.Methods:Chicken ovalbumin was valylated as an immunogen to raise polyclonal antibodies (PcAbs) in rabbits. The population of the pan-specific antibodies recognizing valylated lysine was purified using the chemically synthesized valylated peptides consisting of random amino acids. The specificity of the antibodies was evaluated using ELISA, dot blots, Western blots, and immunohistochemistry (IHC) staining in human epididymis as well.Results:A preliminary and simple strategy to make an anti-valylated lysine PcAb was developed. The recognition of the antibodies to valyllysine was evaluated as pan specific. This was useful for the detection of the newly identified valyl modification using ELISA, dot blots, and Western blots. The antibodies were also successfully utilized in IHC assays, which revealed novel valyllysine modification patterns in epididymis tissues of human.Conclusions:A new antibody tool was provided for the study of lysine valylation. The novel expression patterns of valyllysine in the epididymis suggest that this modification may be involved in sperm maturation.

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简介：在在一样的种类的分开的人口之中的重金属忍耐的差别经常作为本地改编被解释了。在移开stressor以后的差别的坚持显示为增加的忍耐负责的机制是遗传上坚定的。果蝇subobscuraCollin(双翅目：Drosophilidae)人口与重金属污染的不同历史从二个地区被取样，并且与铅(Pb)的不同集中在标准媒介上并且在媒介上在实验室为八代高耸了。为了决定从不同自然人口的苍蝇是否在实验室暴露了到污染Pb的媒介，显示人口在在代上的健康部件的特定的可变性，有铅的不同集中的试验性的组为肥沃，发展时间，和egg-to-adult生存能力是在三代(F2，F5，和F8)的assayed。在污染媒介上，肥沃在以后的代被减少，生存能力被增加，不管人口的环境起源。为两张人口，发展时间显示出与铅一起在媒介上慢下来的一个趋势。更快的开发在以后的代被观察。到到在所有的成年开发的污染，意味着的更高的肥沃，更高的生存能力，和更快的鸡蛋的Preadaptation学习了代，在D被发现。与重金属污染的高水平从地区发源的subobscura。

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简介：这份报纸学习多相的thermoelasticcomposites的有效性质。把免费精力和吉布斯基于Helmholtz多相的composites的单个阶段，有效有弹性的张肌，热扩大的张肌，和特定的热的免费精力借助于体积一般水准被导出这些阶段免费精力。特别强调以经常紧张、经常压力的状况，修改Eshelby的micromechanics理论在被开发被放在有效的特定的热的新分析表情的推导上，在包括之间的相互作用被考虑。作为一个解说性的例子，为三阶段的thermoelastic的有效的特定的热的分析表示合成被介绍。

简介：Thepartialchargesimulationmethodispresentedtosolvethecharacteristicimpedanceofthetransmissionlineofspecificcrosssectionwithanoffsetinnerconductor.Thismethodhasahigheraccuracyduetotheaccuratesatisfactionoftheboundaryconditionontheouterconductor.ThecombinedmethodoftheGausseliminationandoptimizationisusedtosolvetheequationofchargesimulation,anditisaneffectivemethodforincreasingtheaccuracyandassuringtheconvergence.TheGreen’sfunctionsoffivetransmissionlines(i.e,withcircular,elliptic,rectangular,troughandslabconductor)aregiven.

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简介：Theobjectiveofthisstudyistoinvestigatethehemodynamicsinpatient-specificthoracicaorticaneurysmanddiscussthereasonforformationofaorticplaque.A3-Dimensionalpulsatilebloodflowinthoracicaortawithafusiformaneurysmand3mainbranchedvesselswasstudiednumericallywiththeaverageReynoldsnumberof1399andtheWomersleynumberof19.2.Basedontheclinical2-DimensionalCTslicedata,thepatient-specificgeometrymodelwasconstructedusingmedicalimageprocesssoftware.Un...

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简介：China’sCrudeMagnesiumOutputDuringJanuary-FebruaryAccordingtothestatisticsofNonferrousMetalsAssociationofChina,China’soutputofcrudemagne-

简介：Anumericalanalysisofnon-Newtonianfluidflowinnon-planararterywithbifurcationwasperformedbyusingafiniteelementmethodtosolvethethree-dimensionalNavier-Stokesequationscoupledwiththenon-Newtonianconstitutivemodels,includingCarreau,CrossandBinghammodels.Theobjectiveofthisstudyistoinvestigatetheeffectsofthenon-Newtonianpropertiesofbloodaswellascurvatureandout-of-planegeometryinthenon-planardaughtervesselsonthevelocitydistributionandwallshearstress.Theresultsofthisstudysupporttheviewthatthenon-planarityofbloodvesselsandthenon-Newtonianpropertiesofbloodareofimportantinhemodynamicsandplayasignificantroleinvascularbiologyandpathophysiology.

简介：Breastcancerisoneofthemostcommonlydiagnosedmalignanciesandtheleadingcauseofcancerdeathofwomenovertheworld.AlargenumberoffemaleswithbreastcancerinVietnamandotherSoutheastAsian(SEA)countriespresentatanearlyagewithmoreaggressivetumorscomparedwithwomeninAustralia.Despiteexperiencingalowincidencerate,theincreasingincidencerateamongSEAcountriesexceedsthatoftheWesternizedworld.Changesinreproductivefactors,environmentalexposures,andlifestylearethepossiblecausesofthistrend.However,limitedevidenceshowsthatthesefactorsareassociatedwithbreastcancerintheVietnamesepopulation.BreastcancerincidencerateswithinVietnamarenotuniformandappeartobedependentongeographiclocation.FindingsfromthisreviewhaveimportantimplicationsforbreastcancercontrolandtreatmentinVietnam.AgoodunderstandingofthemorphologyofthebreastandthetypeandnatureofbreastcancerspresentinginVietnamisrequiredtofacilitatetheintroductionofaneffectivenationalbreastscreeningprogram.

简介：Newtonian,QuemadaandCassonbloodviscositymodelsareimplementedinordertosimulatetherheologicalbehaviorofbloodunderpulsatingflowconditionsinapatientspecificiliacbifurcation.Theinfluenceoftheappliedbloodconstitutiveequationsismonitoredviathewallshearstress(WSS)distribution,magnitudeandoscillations,non-Newtonianimportancefactors,andviscosityvaluesaccordingtotheshearrate.ThedistributionofWSSonthevascularwallfollowsapatternwhichisindependentoftherheologicalmodelchosen.Ontheotherhand,theWSSmagnitudeandoscillationsaredirectlyrelatedtothebloodconstitutiveequationsappliedandtheshearrate.ItisconcludedthattheNewtonianapproximationissatisfactoryonlyinhighshearandflowrates.Moreover,theNewtonianmodelseemstooverestimatethepossibilityfortheformationofatheroscleroticlesionsoraneurysmsatsitesofthevascularwallwheretheWSSareoscillating.

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简介：食物过敏症是世界范围的一个主要健康问题。桅杆房间在桅杆房间degranulation为需要广泛地被学习的立即的超敏性起一个很重要的作用。在这研究，一条途径被采取在vitro学习敏化的桅杆房间degranulation的特征，它与桅杆房间和动物模型的学习联系了。BALB/c老鼠被几食物变应原分别地使免疫，然后，血和腹桅杆房间在不同时间点被收集。一颗动态决心在桅杆房间和serumalIgE之间被执行。顺序的时间点上的比较分析证明在敏化的BALB/c老鼠在桅杆房间degranulation和IgE抗体titers之间有靠近的巧合。而且，敏化的桅杆房间能在vitro对挑战实现特定的degranulation，有趣，但是仔细，tropomyosins导致了桅杆房间degranulation显示的生气反应。这很类似于在vivo抵抗变应原的IgE。学习在桅杆房间上揭示了一些特征，来自敏化的BALB/c老鼠，在vitro的degranulation。

简介：Bacterialflagellarfilamentcanundergoastress-inducedpolymorphicphasetransitioninbothvitroandvivoenvironments.Thefilamenthas12differenthelicalforms(phases)characterizedbydifferentpitchlengthsandhelixradii.Whensubjectedtothefrictionalforceofflowingfluid,thefilamentchangesbetweenaleft-handednormalphaseandaright-handedsemi-coiledphaseviaphasenucleationandgrowth.Thispaperdevelopsnon-localfiniteelementmethod(FEM)tosimulatethephasetransitionunderad...

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简介：WehavedevelopedandtestedchimericT-cellreceptors(TCR)specificforp185HER2.Intheseexperiments,retroviralvectorsexpressingtheN297orN29ξreceptorswereconstructedinpRET6.AmphotropicviralproducercellswereestablishedintheGALV-basedPG13packagingcellline.Ficollpurifiedhumanperipheralbloodlymphocytes(PBL)werevitallytransducedusinganoptimizedprotocolincorporatingactivationwithimmobilizedanti-CD3/anti-CD28monoclonalantibodies,followedbyviralinfectioninthepresenceoffibronectinfragmentCH296.Transducedcellswereco-culturedwithhumantumorcelllinesthatoverexpress(SK-OV-3)orunderexpress(MCF7)p185HER2toassayforantigenspecificimmuneresponses.BothCD4^+andCD8^+T-cellstransducedwiththeN297orN29ξchTCRdemonstratedHER2-specificantigenresponses,asdeterminedbyreleaseofTh1likecytokines,andcellularcytotoxicityassays.OurresultssupportthefeasibilityofadoptiveimmunothempywithgeneticallymodifiedT-cellsexpressingachTCRspecificforp185HER2.