简介:Objectiwe:InordertodetecttheroleofmonocytesinHSV-2infection,westudiedtheeffectofherpessim-plexVirus-2infectionontheproductionoftumorne-crosisfactor(TNF-α),interleukin-6(IL-6)secretedbymonocytes.Methods:MonocyteswereinfectedbyHSV-2(333Strain).Culturesupernatantswerecollectedat1,3,5,7dayspost-infection.ThelevelsofTNF-α,IL-6weremeasuredbyenzyme-linkedimmunosorbentas-say(ELISA).Results:ThelevelsofTNF-αsecretionbymono-cytessignificantlydecreasedonfirstdaypost-infection.ThelevelsofIL-6significantlydecreasedonfirstandthirddayspost-infection,andthengradu-allyincreasedtothecontrolonseventhdaypost-infection.Conclusions:TNF-αandIL-6productionbymono-cyteswasinhibitedduringHSV-2infection.Thepro-ductionofcytokinesmayplayanimportantroleinherpessimplexviurs-2pathogenicityandimmunity.
简介:Objective:ThisstudyanalyzedtheTlymphocytesandThl/Th2typecytokineprofileshiftintheperipheralbloodofpatientswithrecurrentgenitalherpes(RGH).Methods:Immunofluorescentstainingofcellsurfaceantigenandintracellularcytokines(IL-2,IL-4,IL-12,IFN-r)inperipheralbloodfrom20RGHpatientsand10controlswereanalyzedusingflowcytometrictechniques.Results:RGHpatientshadsignificantlylowerlevelsofCD3^+Tcells,CD4^+TcellsandCD4^+T/CD8^+Tcellsratiocomparedtocontrollevels(P<0.001),andIL-2-producing,IFN-r-producingandIL-12-producingTcellswereincreasedinRGHpatients(CD4^+T:P<0.001,CD8^+T:P<0.05respectively),whereasIL-4-producingTcellswereincreasedinRGHpatientscomparedtocontrols(CD4+T:P<0.05;CDS^+T:P<0.001respectively).Conclusions:RGHpatientshaveTlymphocytesubsetvariationsandThl/Th2cytokinechanges.TheincreaseinTh2cellsThl/Th2imbalancemayhaveimportantimplicationsforRGHpathogenesis.
简介:目的研究单纯疱疹病毒2型(HSV-2)对人单核细胞表达HLA-Ⅱ类抗原(HLA—DR、HLA-DQ)的影响,探讨单核细胞及HLA-Ⅱ类抗原在单纯疱疹病毒2型感染中的作用。方法用单纯疱疹病毒2型(333株)感染人单核细胞,于感染后1、3、5、7天分别收集细胞,用APAAP法检洲单核细胞HLA—DR、HLA-DQ表达水平。结果单纯疱疹病毒2型感染后1天,单核细胞表达HLA-KR、HLA—DQ抗原水平明显降低,以后逐渐升高,至7天达到对照组水平。结论单纯疱疹病毒2型感染早期能抑制单核细胞表达HLA—Ⅱ类抗原,HSV-2抑制单核细胞表达HLA-Ⅱ类抗原可能是HSV-2入侵机体的手段之。HLA-Ⅱ类抗原在单纯疱疹病毒2型致病及免疫过程中可能起重要作用。
简介:目的:研究TGF-β1和MMP-2在病理性瘢痕表皮中的表达及其意义。方法:运用免疫组化法检测TGF-β1和MMP-2在病理性瘢痕表皮中的表达,并与其在表浅性瘢痕、正常皮肤表皮中的表达进行比较,分析其规律。结果:TGF-β1在病理性瘢痕表皮角质细胞中的阳性率及表达强度均较表浅性瘢痕和正常皮肤明显增高(P值均〈0.05),MMP-2在病理性瘢痕表皮中的表达也出现增强,但与表浅性瘢痕、正常皮肤表皮比较,阳性率的差异不具有统计学意义(P〉0.05),表达强度间的差异具有统计学意义(P〈0.05)。结论:TGF-β1和MMP-2在病理性瘢痕表皮中出现不同程度的表达增高,提示它们可能参与了病理性瘢痕的形成,表皮可能是多种细胞因子的重要来源。
简介:目的:检测生殖器疱疹患者外周血中肥大细胞数量、IL-33及IL-6的水平变化,探讨其在生殖器疱疹发病机制中的作用。方法:选择30例生殖器疱疹患者,采用口服伐昔洛韦片治疗,治疗前后流式细胞仪检测外周血中肥大细胞数量,ELISA检测IL-33、IL-6的水平,并与30例健康对照者进行比较。结果:生殖器疱疹患者经伐昔洛韦治疗后痊愈14例(46.7%),显效13例(43.3%),有效3例(10%),无效0例,总显效率为90%,无不良反应发生。治疗前生殖器疱疹患者外周血中肥大细胞数量较健康对照组明显增多(t=29.83,P<0.01),IL-33、IL-6水平升高(t值分别为170.88、25.03,P值均<0.01)。生殖器疱疹患者治疗后外周血中肥大细胞数量较治疗前明显减少(t=17.10,P<0.01),IL-33、IL-6水平较治疗前明显降低(t值分别为163.36、15.61,P值均<0.01)。结论:肥大细胞发挥其天然免疫应答作用,可能通过调节IL-33、IL-6参与HSV-2感染所致生殖器疱疹的发病机制。
简介:Objective:TostudytheexpressionofFasandBcl-2proteinsonTlymphocytesubsetsintheperipheralbloodofrelapsingpatientswithcondylomaacuminatum(CA)andhealthycontrols.Methods:Flowcytometry(permeabizationandstainingprocedurewithconjugatedantibodies)wasused.Results:WeobservedthattheexpressionofFasproteinonCD4^+TlymphocytesubsetofCApatientswassignificantlyhigherthanthatofhealthycontrols(P<0.01).Conclusions:IncreasedexpressionofFasproteinonCD4^+Tlymphocytesubsetmaybeacauseofde-creasedpercentageofCD4^+Tlymphocytesubset.ThisinducestheincreasedratioofCD4^+/CD8^+.
简介:Objective:Tostudytheexpressionofactivatedepi-dermalgrowthfactorreceptor(EGFR)andtranscrip-tionfactorE2F(E2F)inCondylomaAccuminata(CA)patients.Methods:ImmunofluorescenttechniqueswereusedtoinvestigatetheexpressionofactivatedEGFRandE2FinCApatients.Results:TheexpressionofactivatedEGFRonthemembraneofepithelialcellsinCAlesionswassig-nificantlygreatercomparedtoexpressionleversinthecontrolgroup(P<0.01).Moreover,theco-expres-sionofactivatedEGFRandE2Fwassignificantlyin-creasedcomparedtothecontrolgroup(P<0.01).Conclusion:Ourobservationssuggestthatthein-creaseinactivatedEGFRexpressionmaystimulatehyperplasiainCApatientsthroughtheactivationoftranscriptionfactorE2F.
简介:目的观察单纯疱疹病毒2型(HSV-2)对单一核细胞产生肿瘤坏死因子α(TNF-α)和白介素-6(IL-6)的影响,探讨单一核细胞在HSV-2感染中的作用。方法用HSV-2(333株)感染单一核细胞,于病毒感染1,3,5,7日收集细胞培养上清液,用ELISA法检测TNF-a、IL-6的含量。结果HSV-2感染后1日,单一核细胞分泌TNF-α水平显著降低。HSV-2感染后1,3日,单一核细胞分泌IL-6水平显著降低,以后,病毒感染单一核细胞分泌TNF-α、IL-6水平逐渐增高,到第7日达到对照组水平。结论HSV-2能抑制单一核细胞分泌TNF-α和IL-6。而这两种细胞因子在HSV-2致病和机体免疫中起重要作用。
简介:Objective:TostudytheeffectoftraditionalChinesemedicineantiviralcapsulesinthetreatmentofgenitalherpes.Methods:Usingfemaleguineapiggenitalherpesastheanimalmodel,thisstudyusedoraladministrationoftwoformulationsofantiviralcapsules(AC)andobservedtheeffectonvaginalHSV-2titersandvulvarsymptoms.CellcultureswerealsousedtoexaminethedirectinactivationofHSV-2bytheantiviralcapsulesandthesuppressionofHSV-2viathreedrugadministrationmethods.Results:Therewasnosignificantdifferenceofmeanvaginalvirustitersbetweentheantiviralcapsulegroupsandthatofthepositiveacyclovir(ACV)control(P>0.05).Meanvulvarsymptomscoresofthetwoantiviralcapsulegroupswerealsosignificantlylowerthanthatofthesalinenegativecontrolgroupondays2,3,5,7and8(P<0.05)andsimilartothatoftheACVcontrol(P>0.05).CellcultureshowedtheminimuminhibitoryconcentrationsofantiviralcapsulesNo.1andNo.2were0.390625mg/mland1.5625mg/ml,respectively.Conclusion:ThetraditionalChinesemedicineantiviralcapsuleshadsuppressiveeffectsonHSV-2inbothanimalmodelGHandinvitrocellculture.