Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography

(整期优先)网络出版时间:2004-01-11
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NucleosideisthemainclassofactivecomponentsinCordycepssinensis.Thin-layerchromatography(TLC)isoneofthemostcommonlyusedmethodsinpharmacopoeiasforanalyzingchemicalcomponentsofherbalmedicine.SincetheisocraticelutionmethodcannotbeappliedsuccessfullyinTLCanalysisforseparatingallthenucleosidecomponents,thestepwisegradientelutionhasbeendevelopedinthisworktoseparateeightnucleosidestandardswithsuccess.Inthisway,quantitativeanalysesofthesamplesofCordycepssinensiswereachievedviatheproposedTLCprocedurecoupledwiththescanningdensitometrictechniquesofCAMAGandTLCQAmethodsforqualitativeandquantitativeanalysis.