简介:目的分析耳鼻喉科门诊耳呜病例的临床特点。方法对2009年12月~2010年7月在本科门诊就诊的2408例患者进行耳鸣问卷调查,将获得的数据进行统计学分析。结果2408例患者中,年龄6—95岁,平均(47.02±17.05)岁。确诊的耳鸣患者共996例,其中男性388例(38.96%)、女性608例(61.04%),男女耳鸣发生率差异有统计学意义(P=0.0019)。排除4例12岁以下患者,将其余992例患者,根据耳鸣对生活影响的轻重分为4类:无影响149例(占15.02%)、轻度影响480例(占48.39%)、中度影响329例(33.16%)、重度影响34例(3.43%),对生活影响的轻重程度与就诊的关系存在显著相关性(P〈0.0001)。耳鸣持续时间与单双耳发病两者之间存在显著相关性(P〈0.0001)。随机询问44例耳呜患者,发现治疗时间〈1个月者,有效10例、无效14例;1—2个月者,有效6例;〉2个月者,有效8例、无效6例。结论耳鸣的发生率在耳鼻喉科门诊患者中所占比例极高,其中尤以女性多见。双侧耳鸣持续时间更长。随着治疗时间的延长,有效率有增加趋势。(中国眼耳鼻喉科杂志,2013,13:173—175)
简介:AIM:Toexaminetheexpressionofsurvivinandvascularendothelialgrowthfactor(VEGF)duringthedevelopmentofretinalneovascularization(NV)inamousemodel.·METHODS:Awell-characterizedmurinemodelofretinalNVwasusedtostudytheexpressionofsurvivinandVEGF.NVoftheretinawasinducedinmicebyexposureto75%O2frompostnataldayP7toP12,followedbyreturntoroomairfromP12toP17.ExpressionofsurvivinandVEGFproteinwasanalyzedbyImmunohistochemistry.Inaddition,mousemodelofproliferativeretinopathywasanalyzedbyretinalfluoresceinangiographyandquantificationanalysis.·RESULTS:Thenormalmicehadbothsuperfiekalanddeepvascularlayersthatextendedfromtheopticnervetotheperiphery.Inintraocularpressure(IOP)micewerecharacterizedbyrepresentatypicalpatternofpathologicalretinalNV.Therearelessorlittlenucleiofnewvesselsvascularendothelialcellbreakingthroughtheinnerretinalthaninretinopathyofprematurity(ROP)mice,largeclustersofbloodvesselswereadherenttotheinternallimitingmembrane(ILM)(0.27±0.20vs23.38±1.027,t=9.454,P<0.001).DuringtheangiogenicperiodfromP13toP17,survivinandVEGFproteinexpressionincreasedinexperimentalretinascomparedwithcontrolsamples(2.56±0.46vs3.34±0.40,t=17.43,P<0.01:2.18±0.75vs4.34±0.25,t=19.61,P<0.01).ProteinlevelsofVEGFandsurvivnhassignificantlypositivecorrelation(P<0.05,r=0.411).·CONCLUSION:CorrelationwasmadeattheproteinlevelsofsurvivinexpressioncomparedwiththatofVEGFinamurinemodelofretinalNV,whichsuggestsatemporalroleforsurvivinandVEGFinnewvesselformationinresponsetohypoxicstimulation.