简介：Phenoliccompounds,themostabundantsecondarymetabolitesinplants,havereceivedmoreandmoreattentioninrecentyearsbecauseoftheirdistinctbioactivities.Thisreviewsummarizesdifferenttypesofphenoliccompoundsandtheirextractionandanalyticalmethodsusedintherecentreports,involving59phenoliccompoundsfrom52kindsofplants.Theextractionmethodsincludesolid–liquidextraction,ultrasound-assistedextractions,microwave-assistedextractions,supercriticalfluidextraction,andothermethods.Theanalysismethodsincludespectrophotometry,gaschromatography,liquidchromatography,thin-layerchromatography,capillaryelectrophoresis,andnear-infraredspectroscopy.Afterillustratingthespecificconditionsoftheanalyticalmethods,theadvantagesanddisadvantagesofeachmethodarealsosummarized,pointingouttheirrespectivesuitability.Thisreviewprovidesvaluablereferenceforidentificationand/orquantificationofphenoliccompoundsfromnaturalproducts.

简介：目的这研究被设计评估tanshinone的反癌症行动我和tanshinoneIIA，和正常、癌的冒号房间上的tanshinoneIIA的六衍生物。结构活动关系(SAR)分析被进行描出为改进反癌症的tanshinones的结构的修正的意义行动。方法Tanshinone衍生物根据文学被设计并且综合。结肠癌房间上的不同混合物的cytotoxicity被MTT试金决定。tanshinones的Apoptotic活动被流动cytometry(FCM)测量。结果Tanshinone我和tanshinoneIIA两个都在结肠癌房间上展出了重要cytotoxicity。他们在p53+/+结肠癌房间线是更有效的。这也被注意我是的tanshinone的反癌症活动更多的有势力并且选择。tanshinoneIIA(N1和N2)的二衍生物也在结肠癌房间上展出了cytotoxicity。结论tanshinone的反冒号癌症活动我是更多的有势力并且比tanshinoneIIA选择，并且p53依赖者。tanshinoneIIA的结构的修正获得的衍生物在正常和结肠癌房间上展出了更低的cytotoxicity。从位、电子的特征观点，戒指A和呋喃或dihydrofuran的结构的修正在tanshinones影响的基本结构上包围D，这被结束这项活动。A和B戒指的delocalization的增加能提高如此的混合物的cytotoxicity，当一个非平面、小的大小的D戒指区域将提供改进反癌症活动时。

简介：PlatycodinD(PD)，从Platycodonis根值孤立的triterpenoidsaponin，是被显示了在几根癌症房间线有anti-proliferative效果的一副著名中草药。这研究的目的是用proteomics途径与PD在hepatocellular癌HepG2房间的治疗以后在细胞的蛋白质决定变化。房间生存能力用MTT试金被决定。proteome被二维的差别胶化电气泳动和帮助矩阵的激光解吸附作用/电离time-of-flight团spectrometry分析。西方的污点分析被用来证实改变的蛋白质的表示。我们的结果证明PD在集中依赖者和时间依赖者礼貌禁止了HepG2房间的增长。十六蛋白质被识别在对待PD的HepG2房间起来调整包括ATP5HOXCT1，KRT9，CCDC40，ERP29，RCN1，ZNF175，HNRNPH1，HSP27，PA2G4，哲学学士，BANF1，TPM3，ECH1，LGALS1，和MYL6三蛋白质(即，RPS12，EMG1，和KRT1)与PD在治疗以后在HepG2房间减少了。在HSP27和哲学学士的变化被西方的弄污进一步证实。在结论，我们的结果为PD的反癌症活动在行动的机制上打开新灯。

简介：ItrecentlybecomesanimportantandurgentmissionformodernscientificresearchtoidentifyandexplainthetheoryoftraditionalChinesemedicine(TCM),whichhasbeenutilizedinChinaformorethanfourmillennia.SincefewworkshavebeencontributedtounderstandingtheTCMtheory,themechanismofactionsofdrugswithcold/hotpropertiesremainsunclear.Inthepresentstudy,sixkindsoftypicalherbswithcoldorhotpropertieswereorallyadministeredintomice,andserumandliversampleswereanalyzedusinganuntargetednuclearmagneticresonance(NMR)basedmetabolomicsapproachcoupledwithsimilarityanalysis.Thisapproachwasperformedtoidentifyandquantifychangesinmetabolicpathwaystoelucidatedrugactionsonthetreatedmice.Ourresultsshowedthatthosedrugswithsamepropertyexertedsimilareffectsonthemetabolicalterationsinmouseserumandliversamples,whiledrugswithdifferentpropertyshoweddifferenteffects.Theeffectsofherbalmedicineswithcold/hotpropertieswereexertedbyregulatingthepathwayslinkedtoglycometabolism,lipidmetabolism,aminoacidsmetabolismandothermetabolicpathways.Theresultselucidatedthedifferencesandsimilaritiesofdrugswithcold/hotproperties,providingusefulinformationontheexplanationofmedicinalpropertiesoftheseTCMs.

简介：Xingxiong注射(XXI)是叶片白果树摘录和ligustrazine为心血管、脑血管的疾病的治疗作好准备的一个广泛地使用的中国草药的公式。与药理学研究相比，这个公式上的化学分析和质量控制研究是相对有限的。在现在的学习，高效液体层析结合了四极time-of-flight团spectrometry(HPLC-QTOFMS)方法在XXI被用于成分的全面分析。根据破碎规则和以前的报告，三十白果树flavonoids，四白果树萜烯内酯，和一碱被识别。高效液体层析结合了三倍的四极团spectrometry(HPLC-QQQMS)方法然后被使用确定在XXI的十主要成分。方法确认结果显示发达方法有理想的特性，线性，精确和精确性。白果树flavonoids的全部的内容关于22.05-25.51g·mL−1，白果树萜烯内酯数量分别地关于在XXI样品的六批的4.41-8.70g·mL−1。而且，余弦比率算法和距离大小被采用评估XXI样品的类似，并且结果表明了高质量的一致性。这个工作能在Xingxiong注射的质量控制上提供全面信息，它在一个合理质量控制标准的建立是有用的。

简介：WithagreatdifferenceintherapeuticeffectsofMahuang(MH,thestemsofEphedrasinica)andMahuanggen(MHG,therootsofEphedrasinica),chemicaldifferencesbetweenMHandMHGshouldbeinvestigated.Inthepresentstudy,gaschromatography-massspectrometry(GC-MS)-basedplantmetabolomicswasemployedtocomparevolatileoilprofilesofMHandMHG.TheantioxidantactivitiesofvolatileoilsfromMHandMHGwerealsocompared.32differentialchemicalmarkerswereidentifiedaccordingtothevariableimportanceintheprojection(VIP)valueoforthogonalpartialleastsquaresdiscriminantanalysis(OPLS-DA)andPvalueofMann-Whitneytest.Amongthem,chemicalmarkersoftetramethylpyrazine(TMP)andα-terpineolwerequantified.TheircontentsweremuchhigherinmostMHsamplescomparedwithMHG.TheantioxidantassaydemonstratedthatMHhadsignificantlyhigherfreeradical-scavengingactivitythanMHG.AlthoughMHandMHGderivedfromthesamemedicinalplant,therewasmuchdifferenceintheirvolatileoilprofiles.MHsampleshadsignificantlyhighercontentoftworeportedpharmacologicallyimportantchemicalmarkersofTMPandα-terpineol,whichmayaccountfortheirdifferentantioxidantactivities.

简介：ThisstudydevelopedapopulationpharmacokineticmodelforsodiumtanshinoneIIAsulfonate(STS)inhealthyvolunteersandcoronaryheartdisease(CHD)patientsinordertoidentifysignificantcovariatesforthepharmacokineticsofSTS.Bloodsampleswereobtainedbyintensesamplingapproachfrom10healthyvolunteersandsparsesamplingfrom25CHDpatients,andapopulationpharmacokineticanalysiswasperformedbynonlinearmixed-effectmodeling.Thefinalmodelwasevaluatedbybootstrapandvisualpredictivecheck.Atotalof230plasmaconcentrationswereincluded,137fromhealthyvolunteersand93fromCHDpatients.Itwasatwo-compartmentmodelwithfirst-orderelimination.Thetypicalvalueoftheapparentclearance(CL)ofSTSinCHDpatientswithtotalbilirubin(TBIL)levelof10μmol(L?1was48.7L(h?1withinterindividualvariabilityof27.4%,whereasthatinhealthyvolunteerswiththesameTBILlevelwas63.1L(h?1.Residualvariabilitywasdescribedbyaproportionalerrormodelandestimatedat5.2%.TheCLofSTSinCHDpatientswaslowerthanthatinhealthyvolunteersanddecreasedwhenTBILlevelsincreased.Thebootstrapandvisualpredictivecheckconfirmedthestabilityandvalidityofthefinalmodel.TheseresultssuggestedthatSTSdosageadjustmentmightbeconsideredbasedonTBILlevelsinCHDpatients.

简介：HyoscyamiSemen,thematuredriedseedofHyoscyamusnigerL.,haslongbeenusedasatraditionalChinesemedicinetotreathumandiseases.HyoscyamiSemenisfoundinlocalmarketsinChina.Inmarkets,sellersandbuyerscommonlyinadvertentlymixtheseedsofH.nigerwiththeseedsofrelatedspeciessuchasHygrophilasalicifolia(Vahl)Nees,AstragaluscomplanatusR.Br.,CuscutaaustralisR.Br.,CuscutachinensisLam.,andImpatiensbalsaminaL.becauseoftheirsimilarmorphologiesorsimilarnames.Thus,developingareliablemethodfordiscriminatingH.nigerseedsfromitsadulterantsisnecessarytoreduceconfusionandensurethesafeuseofHyoscyamiSemen.Thepresentstudywasdesignedtoevaluatetheefficiencyofhigh-resolutionmeltinganalysiscombinedwithDNAbarcoding(Bar-HRM)withinternaltranscribedspacer2todiscriminateH.niger.OurresultsshowthatBar-HRMsuccessfullyidentifiedtheadulterantsanddetectedtheproportionofH.nigerDNAextractwithinanadmixture.Inparticular,HRMdetectedH.nigerDNAextractinA.complanatusDNAextractatconcentrationsaslowas1%.Inconclusion,theBar-HRMmethoddevelopedinthepresentstudyforauthenticatingH.nigerisrapidandcost-effective.ItcanbeusedinthefuturetoguaranteethepurityofHyoscyamiSemenfortheclinicaluse.

简介：ThepresentstudyinvestigatedthechemicalcompositionofethylacetateextractsfromanendophyticactinomyceteStreptomycessp.A0916anditshostPolygonumcuspidatum.Acomparativeanalysisoftheantimicrobialandantioxidantpropertiesoftheextractswasalsoconducted.32compoundsofP.cuspidatumand23compoundsofStreptomycessp.A0916wereisolatedandidentifiedbyGC/MS.Antimicrobialactivitiesoftheextractswereevaluatedusingeightmicrobialstrains(3Gram-positivebacteria,3Gram-negativebacteria,and2fungi).TheStreptomycessp.A0916extractsshowedawiderangeofantimicrobialactivitiesandpresentedgreaterantimicrobialeffectivenessthantheP.cuspidatumextracts.Theminimuminhibitoryconcentration(MIC)ofStreptomycessp.A0916extractsagainsttheampicillin-resistantstrainEnterococcusfaeciumSIIA843was32μg·mL-1.Furthermore,theextractshadgreaterantimicrobialeffectagainstGram-positivebacteriathanGram-negativebacteria.Finally,theantioxidantactivityoftheStreptomycessp.A0916extractswasequaltothatoftheP.cuspidatumextracts.Inconclusion,ourresultssuggestthattheendophyticactinomycetesofthemedicinalplantsareanimportantsourceofbioactivesubstances.

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