简介：这研究被设计从A孤立endophytic真菌。mongholicus(在东北中国成长)决定他们是否能生产bioactive代谢物。endophytic真菌的四紧张(拉紧16，17，23和75)成功地从A被孤立。用表面消毒方法的mongholicus。根据ITS-rDNA序列分析，拉紧16和75作为镰刀霉oxysporum被识别，并且拉紧17和23作为Bionectriaochroleuca被识别。我们使用了Box-Behnken设计(BBD)优化液体发酵条件并且获得最大的房间干燥重量(CDW)产量。最佳的参数在下列试验性的条件下面被获得：第28i的温度口香糖的年龄。与那，新鲜口香糖增加了拍的成功。第二，本地人比较喜欢在温暖的季节期间拍，吗我们由决定Xac生长在30略潤潭慮?楤業畮慴?是最好的验证了哪个?桓杩汥慬????????????????????????楦浲獵??‰???堠湡桴浯湯獡？？

简介：一个总数3611真菌孤立从在季风，冬季和夏天期间从7药用的植物孵化的4200个叶片断被恢复季节。这些真菌孤立属于teleomorphicAscomycota(23.5%)，生产conidiomata(17.4%)的歪像的Ascomycota，没有conidiomata(46.9%)的歪像的Ascomycota，Zygomycota(1.42%)和无菌的形式(10.6%)。Chaetomiumglobosum，曲霉属菌尼日尔，Aureobasidiumpullulans，Curvularialunata，镰刀霉spp，毛丛spp，Pestalotiopsisspp，Trichodermaviridae，Cladosporiumcladosporioides，经常从超过一主人植物被孤立。endophytic的数字孤立比在季风和夏天季节在冬季是更高的。

简介：Endophyticfungiarewidelyfoundinalmostallkindsofplants.Manyendophyticfungicanproducesomephysiologicalactivecompounds,whicharesametooranalogtothoseisolatedfromtheirhosts.Producingphysiologicalactivecompoundsthroughmicrobialfermentationcangiveanewwaytoresolveresourcelimitationandtofindoutalternativesource.Throughthemethodsoforganicsolventextraction,thinlayerchromatography(TLC)andcolumnchromatography,compoundIwasisolated,purifiedfromtheliquidfermentationmetabolitesofthetaxoids-producedendophyticfungi(Alternaria.alternatavar.taxi1011Y.XiangetLUAn-guo)thatwasscreenedfromthebarkofTaxus.cuspidataSieb.etZucc..CompoundIwasidentifiedasonekindoftaxoidstypeIII,basedontheanalyzingresultsbyusingthemethodsofultravioletspectroscopy(UV),infraredspectroscopy(IR),massspectrometry(MS)andnuclearmagneticresonancespectroscopy(NMR).Thisstudyprovidesacompletedmethodforseparationandpurificationoftheendophyticfungiaswellasstructureidentificationofitsfermentationmetabolite

简介：ThepresentstudyinvestigatedthechemicalcompositionofethylacetateextractsfromanendophyticactinomyceteStreptomycessp.A0916anditshostPolygonumcuspidatum.Acomparativeanalysisoftheantimicrobialandantioxidantpropertiesoftheextractswasalsoconducted.32compoundsofP.cuspidatumand23compoundsofStreptomycessp.A0916wereisolatedandidentifiedbyGC/MS.Antimicrobialactivitiesoftheextractswereevaluatedusingeightmicrobialstrains(3Gram-positivebacteria,3Gram-negativebacteria,and2fungi).TheStreptomycessp.A0916extractsshowedawiderangeofantimicrobialactivitiesandpresentedgreaterantimicrobialeffectivenessthantheP.cuspidatumextracts.Theminimuminhibitoryconcentration(MIC)ofStreptomycessp.A0916extractsagainsttheampicillin-resistantstrainEnterococcusfaeciumSIIA843was32μg·mL-1.Furthermore,theextractshadgreaterantimicrobialeffectagainstGram-positivebacteriathanGram-negativebacteria.Finally,theantioxidantactivityoftheStreptomycessp.A0916extractswasequaltothatoftheP.cuspidatumextracts.Inconclusion,ourresultssuggestthattheendophyticactinomycetesofthemedicinalplantsareanimportantsourceofbioactivesubstances.

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