简介:适当大小被需要控制钙离子内容并且在好矿物质粒子之中减少相互作用。在这份报纸,闪锌矿/硅石相互作用被希腊语的第六个字母潜在的大小和新奇希腊语的第六个字母潜在的分布(ZPD)学习在钙离子答案的不同集中的测量方法。到分子的学习矿物质表面和原子吸收状态,精力散光谱学(版本)和扫描电子显微镜(SEM)被使用。在矿物质表面上吸附的筹款试剂被相互作用地位分析。结果证明当钙离子集中在溶液高时,筹款试剂的那吸附不是导致的。减少钙离子集中是有效方法在肉减少粒子相互作用。特定的方法是钠碳酸盐的适当数量被增加进使过饱和石膏的答案。矿物质相互作用能被测量ZPD解释。
简介:Effectsofstartingmaterialsandfourdispersants(STP,SHP,FDNandFS60)onZetapotentialandrheologicalbehaviorofaluminabasedULCcastablesmatrixwereinvestigated.Theresultsshowthat:characteristicsofsilicafumeandaluminacementsplayaveryimportantroleinZetapotentialandviscosityofsuspensionsofthecastablesmatrix;thedispersantsSTPandSHPcanchangeZetapotentialvaluesofthematrixsuspensionsremarkably;thefourdispersantscaneffectivelyimprovetherheologicalpropertiesofmatrixsuspensions.Forthepointoflowerviscosityofthematrixsuspensions,thesuitableadditionsofthethreedispersants(SHP,FDNandFS60)areabout0.2%whilethatofSTPisabout0.3%.
简介:BACKGROUND:TheprogressivedegenerationofdopaminergicneuronsinParkinson’sdiseaseisassociatedwithanactivatedglialreaction,combinedwithaninflammatoryprocess.Theseresponsesleadtotheproductionofcytokines,suchasinterferon-γ,tumornecrosisfactor-α(TNF-α),andinterleukin-1β.Inaddition,14-3-3proteinisacomponentofLewybodiesinParkinson’sdisease.OBJECTIVE:Toobservetheexpressionof14-3-3γandζprotein,aswellasTNF-α,inmousemicroglia,aswellaschangesafterlipopolysaccharide(LPS)activation.Toinvestigatepossiblemechanismsofdopaminergicneuronalinjuryduetoactivatedmicroglia.ToandclarifytheimmuneresponsemechanismsofParkinson’sdisease.DESIGN:Randomizedcontrolledobservation,cellstudy.SETTING:LaboratoryofDepartmentofNeurology,theAffiliatedUnionHospitalofTongjiMedicalCollege,HuazhongUniversityofScienceandTechnology.MATERIALS:TheBV-2immortalizedmurinemicrogliacelllinewaspurchasedfromChinaUnitcellcenter.LPSwasprovidedbySigmaCompany.CellcultureswerepurchasedfromGibco.Phospho-(Ser)14-3-3bindingmotifantibodywaspurchasedfromSantaCruzBiotechnologies.FITCwasprovidedbyLinfeiBiotechnology,Wuhan,China.TNF-αELISAwasprovidedbyJingmeiBiotechCo,Wuhan,China.TheflowcytometerwasprovidedbyBectonDickinson,Canada.METHODS:ThepresentexperimentwasperformedattheLaboratoryofDepartmentofNeurology,theAffiliatedUnionHospitalofTongjiMedicalCollege,HuazhongUniversityofScienceandTechnologyfromApriltoDecember2006.Themicroglialcellline,BV-2,wasculturedinvitroandstimulatedwithLPSfor2,6,12,and24hours.BV-2cultureswithoutLPSwereusedascontrols.MAINOUTCOMEMEASURES:Expressionof14-3-3γproteinwasdetectedbyflowcytometry.14-3-3ζpercentageexpressionandthemeanfluorescenceintensitywasdetectedbyimmunofluorescence.TNF-αexpressionwasdetectedbyELISA.RESULTS:14-3-3γproteinexpressionanalysis:followi