简介：Toelucidatethemechanismofphotodynamicdamageofcells,theeffectofHPDpluslightontranscriptlonalectlvltyInthemucleusIsolatefromthenormalratliverwasstudiedinvitorby3H-UTPincorporationintoRNA.MeasurementsoffluorescencespectrumshowedthatHPDwasboundtothenucleusanditsfluorescenceIntensityIncreasedwiththeIncreaseofHPDconcentration.TheexperimentalresultsIndicatedthatnochangescouldbeobservedwheneitherHPDorlightwasusedalone.WhereasthenucleartranscriptionactivitywasfoundtobeinhibitedsignificantlybybothHPDandlighttreatment,andthedegreeofInhibitionwasdependentontheHPDconcentrationandthetimeofexposuretolight.Aftertreatmentby3μg/mlHPD,theinhibitionrateofthenucleartranscriptionactivitywas23%,45%,69%,80%and90%,respectivelyforlightexposureof2,5,10,20and30minutes.Ourresultssuggestedthatdose-dependentdecreasesinthenucleartranscriptionactivity,andmarkedinhibitionofthe

简介：AmethodisdescribedwhichpermitstransmissionelectronmicroscopeofsinglecellstreatedwithHpDpluslasermicroirradiation.Thepreselectedsinglecellthatwasirradiatedbylaserunderlightmicroscopeandfollowedfixation,embeddedandsectioningisexaminedunderelectronmicroscope.Theresultsdemonstratedthatatthelightdoseof1.88ml/μm2notonlytheirradiatednucleolusappearedtransparentregion,buttheotherpartssuchasnon-irradiatedmitochondriaincytoplasmcanalsobedamaged.Whenpartialcytoplasmisirradiatedwiththelightdoseof4.50ml/μm2,thedamagesappearinallcytoplasm,butthereislittlechangeinthenucleus.Theexperimentalresultsalsodemonstratethatcytoplasmismoresensitivethannucleus.ItisthemitochondriaincytoplasmthatareverysensitivetoHpDpluslaser.

简介：随着海道测量生产数据库（以下简称HPD）技术在海图生产中的应用,传统的航海图书产品的生产流程和生产模式正在发生革命性变革。随之带来的海图改正通告编辑工作的变化同样也不例外。文中对改正通告现有工作模式进行分析,结合HPD技术的特点,探讨海图改正通告工作未来与之相适应的改革方法。

简介：摘要：现代化背景下医疗体制改革深入，加强内部控制成为趋势，医院内部成本控制要通过合理的管理体系实现。国家近年以来陆续出台了成本控制政策，认为医院应该采取精细化管理来重视医院的成本管理，统筹医疗体系的管理内容促进管理体系的变革，让医院发展符合市场经济发展的需要。HPD能够提高医院的整体管理质量，解决医院采购和物流管理中出现的问题，能够减轻成本消耗，减轻工作人员的工作量，保证医院经济活动的健康展开。