简介：Objective:Toexploretheeffectofmorphologicalchangesinthedevelopmentofcondylomaacuminatum(CA)MaterialsandMethods:LesionsinfivepatientswithCAwereobserved.Results:Uponelectronmicroscopy,themostcharacteristicfeatureofthelesionsimportantfordiagnosis,wasthepresenceofdistinctperinuclearvacuolizations,orso-calledkoilocytes,amongsomeepithelialcells.Thesecellspossessedhyperchromaticnuclei,swollenmitochondria,dilatedendoplasmicreticulumanddissolvedglycogen.Therewereinterchromatingranulesandperichromatingranulesinsomenuclei.Moreoversomevirusparticleswerealsoseeninthenucleiofsomeinfectedcells.Conclusions:Theultrastructuralfindingsmaybeusedtohistopathologicallyexplainthepathogenesisandmechanismofthisdisease,anditishelpfulfordiagnosisofCA.

简介：Abstract:Thepossiblecausesofcondylomaacuminatarecurrencearesummarized:suchaspatientswithcellularimmunedeficiencies,physicaltherapytriggeringsubclinicalinfectivefoci,sowingofthevirusparticles,andinsufficienttherapy.Correspondingpreventivemeasuresareaddressed,including:immunomodulatorsimprovingcellularimmunity,ensuringtherangeanddepthofphysicaltherapy,andtreatingsexualpartnerssimultaneously.

简介：Objective:Infectionofhumanpapillomavirusincondylomaacuminatum(CA)wasdetectedbyrealtimefluorescencequantitativePCR(FQ-PCR)technique.Methods:SpecimensofCA-DNAquantificationfrom94caseswereexaminedbyrealtimeFQ-PCRtechniqueand32caseswerecomparedwiththesamemethodafter10-daystreatment.Results:CA-DNAwasfoundinallpatients,withanaverageof4.0×10^6copies/ul.After10daysoftreatment,theaveragewas2.1×10^5copies/ul.TherewasasignificantdifferenceintheaverageamountofCA-DNAbeforeandafterthetreatment.Conclusion:RealtimeFQ-PCRisagoodmethodforexaminingCA-DNAamountanditcandirectthetreatmentofCA.

简介：Objective:TostudytheimmunologicalmechanismsofCondylomaacuminata(CA)throughinvestigatingTlymphocytesubsetlevelsandcytokineprofileintheperipheralbloodofpatientswithCondylomaAcuminata.Methods:Tricolorandbicolorimmunofluorescentstainingantibodyofcellsurfaceantigenandintracel-lularIL-2,IL-4,IL-12,IFN-γinCD4^+andCD8^+T-lymphocytesfrom20patientswithCAwereperformedandfollowedbyflowcytometry.Results:ThenumberofCD3^+T,CD4^+T-lymphocytescellsandCD4^+/CD8^+Tcellsratioweresignificantlydecreased(P<0.01)inpatientswithCAComparedtocontrols,andIL-2,IL-12,IFN-γproductioninCD4^+Tcellswasdecreased(P<0.01),IL-4andIFN-γproduc-tioninCD4^+Tcellswasnotsignificantlydifferent(P>0.05),whileIL-2andIL-12productioninCD8^+Tccellswasdecreased(P<0.01),whereasIFN-γandIL-4pro-ducinginCD4^+Tcellswereofnosignificantlydifference(P>0.05).Conclusions:TherewasanimbalanceofTlympho-cytesubsets,Th1/Th2cytokinesandTc1/Tc2intheperipheralbloodofCApatients,whichmayplayanimportantroleinthepathogenesisandprogressionofCA.

简介：无

简介：Objective:Todetecttheactivatedexpressionoftelomeraseincondylomaacuminatalesionsinlow-risk(6/11)andhigh-risk(16/18)humanpapillomavirus(HPV)infectionandexaminetheroleplayedbytelomeraseintheoccurrence,developmentandcarcinomatouschangeofcondylomaacuminata.Methods:AssayingtheexpressionoftelomeraseandthetypeofHPVindamagedskinof42CApatientsandnormalskinof30healthycontrolindividualsthroughtelomeraserepeatamplificationprotocol(TRAP)andpolymerasechainreaction(PCR).Results:Inallthenormalskincontrols,PCRforHPVwasnegativeandonly16.7%ofsampleswerepositiveforTLMAexpression;inCAlesions,HPVtestingwaspositiveinall(32caseswerelow-risk,3werehigh-risk,and7wereofmixedtype)andallwerepositiveforTLMAexpression.Conclusion:TLMAmaybeactivatedbyHPVinfection,andinturncausethehyperplasiaofepidermalcells.ItwasalsoindicatedthatHPV,especiallyhighrisktypes,canactivateTLMA.TheactivationofTLMAmayplayanimportantroleinabnormalhyperplasiaandcarcinomatouschangesinCAlesions.

简介：Objective:Toinvestigatetheeffectofactivation-in-ducedcelldeath(AICD)oncellularimmunefunctioninthecondylomaacuminatum(CA).Methods:Peripheralbloodmononuclearcells(PBMC)wereisolatedfromnormalhealthyindividuals(controlgroup)andpatientswithCA.PBMCwereculturedwithPHA-Pfor48hinvitro.ApoptosisofthePBMCwasdetectedbyflowcytometry.Supernatantcytokines(IL-2andIL-10)wereassayedbyELISA.Results:TherateofPBMCapoptosisinbothCAgroupandcontrolgroupinfreshPBMCwasverylowandsimilarinbothgroups(P>0.05).TherateofPBMCapoptosiswithintheCAgroupwasnoticeablyincreasedcomparedtothatofthecontrol(P<0.001)afterPBMCwereculturedfor48h.ThelevelofIL-2wassignificantlylowerintheCAgroupthaninthecontrolgroup(P<0.001),ThelevelofIL-10wassignificantlyhigherintheCAgroupcomparedtothecontrolgroup(P<0.001).Conclusion:StudyresultsindicatethatAICDmayaffectcellularmediatedimmunefunctionandplayanimportantroleinthepathogenesisofCA.

简介：Objective:ToevaluatethetherapeuticeffectofNd:YAGlaserunderurethroscopeforthetreatmentofintraurethralCandylomaAcuminata(CA)inmales.Method:Nd:YAGlaserunderurethroscopewasemployedtoremovethewarts.Result:SeventeenpatientsoutofeighteencaseswithCAwerecuredafterthefirstprocedure.Thecureratewas94.4%.Forsecondtreatment,thecureratewas100%andnorelapseoccurredafterobservationfor12-24months.Conclusion:Nd:YAGlaserbyurethroscopeisaverygoodmethodforthetreatmentofintraurethralCA.

简介：Objective:TostudytheexpressionofFasandBcl-2proteinsonTlymphocytesubsetsintheperipheralbloodofrelapsingpatientswithcondylomaacuminatum(CA)andhealthycontrols.Methods:Flowcytometry(permeabizationandstainingprocedurewithconjugatedantibodies)wasused.Results:WeobservedthattheexpressionofFasproteinonCD4^+TlymphocytesubsetofCApatientswassignificantlyhigherthanthatofhealthycontrols(P<0.01).Conclusions:IncreasedexpressionofFasproteinonCD4^+Tlymphocytesubsetmaybeacauseofde-creasedpercentageofCD4^+Tlymphocytesubset.ThisinducestheincreasedratioofCD4^+/CD8^+.

简介：Objective:Tostudytheexpressionofactivatedepi-dermalgrowthfactorreceptor(EGFR)andtranscrip-tionfactorE2F(E2F)inCondylomaAccuminata(CA)patients.Methods:ImmunofluorescenttechniqueswereusedtoinvestigatetheexpressionofactivatedEGFRandE2FinCApatients.Results:TheexpressionofactivatedEGFRonthemembraneofepithelialcellsinCAlesionswassig-nificantlygreatercomparedtoexpressionleversinthecontrolgroup(P<0.01).Moreover,theco-expres-sionofactivatedEGFRandE2Fwassignificantlyin-creasedcomparedtothecontrolgroup(P<0.01).Conclusion:Ourobservationssuggestthatthein-creaseinactivatedEGFRexpressionmaystimulatehyperplasiainCApatientsthroughtheactivationoftranscriptionfactorE2F.

简介：Abstract:Thisstudyinvestigatedtherelationshipbetweenhumanpapillomavirus(HPV)genotypeandexpressionofp53andp21^WAH1.Expressionofp53andp21^WAH1in35casesofcondylomaacuminatumspecimensinfectedbyHPV6/llandHPV16/18werestudiedusingimmunohistochemicalstaining.Allspecimensofthecondylomaacuminatumcasewerepositiveforexpressionofp53andp21^WAH1.Theexpressionofp53incondylomaacuminatuminfectedbyHPV16/18wassignificantlylowerthanthatinspecimensinfectedbyHPV6/ll.However,expressionofp21wAHbetweenthetwogroupswasnotsignificantlydifferent.Expressionofp53incondylomaacuminatumislikelyrelatedtoHPVgenotype,expressionofp21^WAH1wasnotrelatedtoHPVgenotype.

简介：Objective:Tostudytheexpressionlevelsofplatelet-derivedgrowthfactor(PDGF)andgranulocytecolony-stimulatingfactor(G-CSF)inperipheralbloodandtheirroleinthepathogenesisofCondylomaacuminatum(CA).Methods:Seraweretakenfrom70patientswithCondylomaacuminatumandcomparedwith35healthycontrols.PDGFandG-CSFinserumwerequantitatedusingadualantibodysandwichenzyme-linkedimmunoabsorbentassay(ELISA).Results:SerumconcentrationsofPDGFandG-CSFweresignificantlyincreasedinpatientswithCondylomaacuminatum(CA)comparedtocontrols(P<0.001andP<0.005respectively).SerumlevelsofPDGFandG-CSFcorrelatedwithclinicalseverityofCA,butnosignificantdifferencewasobservedbetweendifferentdurationofdiseasegroups.AsignificantpositivecorrelationwasnoticedbetweenneutrophilcountandG-CSFlevels(γ=0.38,P<0.001),andtheneutrophilcountshowednosignificantcorrelationwithPDGF.Conclusion:TheresultsindicatedthatincreasedexpressionofPDGFandG-CSFinperipheralbloodmightbeinvolvedinpathogenesisofCA.