简介:目的探索不同f2/f1比值对DPOAEs幅值的影响,寻找最佳的测试参数,以得到最大的DPOAEs测值.方法对12例(24耳)正常青年人进行不同f2/f1比值条件下的DPOAEs幅值测试.结果当f1/f1=1.220时,DPOAEs幅值最大(P<0.05或P<0.01).当f2/f1=1.232时,除了f2=2002Hz处以外,其DPOAEs幅值与f2/f1=1.220时无显著性差异(P>0.05).其他f2/f1值的DPOAEs幅值均较低,多数测值与f2/f1=1.220时相比均有显著性差异(P<0.05或P<0.01).结论f2/f1=1.220~1.232时,DPOAEs测值最大,此范围为最佳测试参数值.
简介:SincetheinventionofRubylaserbyMaimanin1960s,manykindsoflasersareusedwidelyindentistry.Ithasbeenprovedthatlaserirradiationcanproduceacidetchinglikesurface,thusstrengthenthebondofmaterialtoenamel.Buttheheatgeneratedduringirradiationproducesdamageondentalpulp,whichlimitsthelaserapplicationsondentalhardsurfaces.
简介:Objective:TodirectionallyclonetheomplgenefromChlamydiatrachomatis(Ct)FGenotypeontoaplasmidvectorforconstructingarudimentaryDNAvaccine.Methods:ThecompleteomplgenefromgenomicDNAofCtFgenotypewildspecieswasamplifiedwithprimersdesignedbycomputer.Therecombinantgenewasobtainedbyrestrictionenzymecutting,linkingthegenewiththeplasmidvectorinvitro,transformingtherecombinantgeneintobacteria,andextractingtheDNAfromthebacteria.Results:DNAextractingfromthebacteriawascomposedoftheimplgeneandplasmid,whichisidentifiedbythreemethodsofsingularrestrictiveenzymecutting,doublerestrictiveenzymecuttingandPCR.Conclusion:CloningoftheomplgenefromtheCtFgenotypemeansthatarudimentaryDNAvaccinewassuccessfullyconstructed.
简介:目的对520份中药说明书中与安全用药关系最直接的不良反应、禁忌、注意事项3项内容进行调查,评价分析目前中药说明书规范、完善的情况.方法依据对药品说明书的规定,收集了5家医院的中药说明书;520份,按剂型分类对调查的5项内容进行统计.结果在520份中药说明书中,同时注明3项内容的有54份,占总数10.6%;分别注明不良反应的有62份,占总数19.83%;注明禁忌的有82份,占总数25.65%;注明注意事项的有257份,占总数74.06%.结论药品说明书是指导临床正确使用药品的技术性资料和依据,制药企业应按对中药说明书的规定,规范、完善说明书的内容,保证药品质量,保障用药安全.
简介:Thehousedustmites(Dermatophagoidesfarinae,Derf)arethemajorsourceofaeroallergensimplicatedintheexpressionofatopicdisorders,includingasthma,allergicrhinitisandatopicdermatitis.Inparticular,strongcircumstantialevidencesuggeststhathousedustmiteantigensareimportantprecipitatingfactorsofasthma.Manyhousedustmiteallergensareproteasesthatcanelicitairwayinflammationbystimulatingthereleaseofcytokinesfrombronchialepithelialcells.ToinvestigatewhetherDerfallergenproteasesinducedcytokineproductionfromtheepithelialcelllineBEAS-2B,BEAS-2Bcellswereculturedwith4differentconcentrationsofDerf(0.02,0.2,2,20μg/ml)for24-96h,afterwhichsupernatantswereassayedforinterleukin(IL)-6andIL-8withELISA.Reversetranscription-PCRwasalsoperformed.ThecellsheetswereintactthroughouttheobservationincontrolgroupwithoutanyexposuretoDerfantigen.IntheexperimentalgroupscellstreatedwithDerfallergenshowedchangesintheanchoragestatusofthemonolayer.Therewasasignificantincreaseinthelevelofcytokineproductioncomparedwiththeuntreatedsample.ThereleaseofIL-6andIL-8increasedinaconcentration-dependentmanner(P<0.05,respectively)withtheadditionofincreasingdosageofDerftothecellsheets.LevelsofIL-6andIL-8begantoriseat24hand48hafterallergenexposure,andtheyincreasedsignificantlyinthesupematantsat72hand96h.AtthesametimetheconcentrationdependenceofinductionofIL-6andIL-8expressionaswellasanincreaseintheexpressionofIL-6andIL-8mRNAmanifestedevidently.HDM-inducedairwayinflammationmayincludeDerf-mediatedreleaseofinflammatorymediators,andtheproteolyticactivityofanallergenmaystimulatethereleaseofproinflammatorycytokinesfromhumanbronchialepithelium.ItissuggestedthatIL-6andIL-8productionbybronchialepithelialcellsmayplayaroleinthepathogenesisofallergicasthma.
简介:Objective:ToinvestigatetheexpressionofE2FandBcl-2andtheclinicopathologicalsignificanceinhepatocellularcarcinoma.Methods:TheexpressionsofE2F-3andBcl-2in74patientswithhepaticcarcinoma,paracarcinomaand15patientswithlivercirrhosisweredetectedbyS-Pimmunohistochemicalstaining.Results:TheexpressionofE2Finhepaticcarcinomawassignificantlyhigherthanthatinparacarcinomaorlivercirrhosis(P<0.005),theexpressionofBcl-2inhepaticcarcinomawassignificantlyhigherthanthatinparacarcinoma(P<0.005),inwhichBcl-2expressionwaslowerthaninlivercirrhosis(P<0.05).TheexpressionofE2F-3wasrelatedwithhistologicalgrade,tumorsize,andtheexpressionofBcl-2wasrelatedwithhistologicalgrade,tumorsizeandtumornumber.TherewascorrelationbetweentheexpressionofE2F-3andBcl-2inhepaticcarcinoma.Conclusion:E2F-3andBcl-2expressionmayplayanimportantroleindevelopment,progressionandcellapoptosisoftumor.
简介:Objective:Tostudytheexpressionofactivatedepi-dermalgrowthfactorreceptor(EGFR)andtranscrip-tionfactorE2F(E2F)inCondylomaAccuminata(CA)patients.Methods:ImmunofluorescenttechniqueswereusedtoinvestigatetheexpressionofactivatedEGFRandE2FinCApatients.Results:TheexpressionofactivatedEGFRonthemembraneofepithelialcellsinCAlesionswassig-nificantlygreatercomparedtoexpressionleversinthecontrolgroup(P<0.01).Moreover,theco-expres-sionofactivatedEGFRandE2Fwassignificantlyin-creasedcomparedtothecontrolgroup(P<0.01).Conclusion:Ourobservationssuggestthatthein-creaseinactivatedEGFRexpressionmaystimulatehyperplasiainCApatientsthroughtheactivationoftranscriptionfactorE2F.
简介:Havingbeenpassedfor160generations,acelllinedesignatedasH22-F25/LwasestablishedfromamurinetumorlymphaticmetastatlcmodelH22-F25whichhadbeensetupinourcollege.Thecelllinewasinsuspensionculturewitharapidproliferationandstablegrowth.Thepeaktuneofcelldivisionandproliferationwas48and96hoursafterculture.Inaweek,thecellnumberwasIncreasedby25tunes.H22-F25/Lstillkeepsthefeaturesofapoorlydifferentiatedcancer.Itstumorinducingrate(invivo)was100%in615mice.Lymphnodemetastasisratewas50%andpulmonarymetastasisrate10%.H22-F25/LIsapopulationofheterogenetlctumorcellsIncluding2stemcelllines(themodelnumberofchromosomesbeing43in40%tumorcellsand86in32%)andsomesidelines.ThecommonmarkerchromosomesM1,M2,M3andM4werepresentinallstemandsidelines.
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