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简介：Breastcancerisoneofthemostcommonlydiagnosedmalignanciesandtheleadingcauseofcancerdeathofwomenovertheworld.AlargenumberoffemaleswithbreastcancerinVietnamandotherSoutheastAsian(SEA)countriespresentatanearlyagewithmoreaggressivetumorscomparedwithwomeninAustralia.Despiteexperiencingalowincidencerate,theincreasingincidencerateamongSEAcountriesexceedsthatoftheWesternizedworld.Changesinreproductivefactors,environmentalexposures,andlifestylearethepossiblecausesofthistrend.However,limitedevidenceshowsthatthesefactorsareassociatedwithbreastcancerintheVietnamesepopulation.BreastcancerincidencerateswithinVietnamarenotuniformandappeartobedependentongeographiclocation.FindingsfromthisreviewhaveimportantimplicationsforbreastcancercontrolandtreatmentinVietnam.AgoodunderstandingofthemorphologyofthebreastandthetypeandnatureofbreastcancerspresentinginVietnamisrequiredtofacilitatetheintroductionofaneffectivenationalbreastscreeningprogram.

简介：Precisionmedicineandpersonalizedtherapyarereceivingincreasedattention,andmolecular-subtypeclassificationhasbecomecrucialinplanningtherapeuticschedulesinclinicalpracticeforpatientswithbreastcancer.Humanepidermalgrowthfactorreceptor2(HER2)isassociatedwithhigh-gradebreasttumors,highratesoflymph-nodeinvolvement,highriskofrecurrence,andhighresistancetogeneralchemotherapy.AnalysisofHER2expressionishighlyimportantfordoctorstoidentifypatientswhocanbenefitfromtrastuzumabtherapyandmonitortheresponseandefficacyoftreatment.Inrecentyears,significanteffortshavebeendevotedtoachievingspecificandnoninvasiveHER2-positivebreastcancerimaginginvivo.Inthiswork,wereviewedexistingliteratureonHER2imaginginthepastdecadeandsummarizedthestudiesfromdifferentpointsofview,suchasimagingmodalitiesandHER2-specificprobes.WeaimedtoimprovetheunderstandingonthetranslationalprocessinmolecularimagingforHER2breastcancer.

简介：目的将在对待matrine的K562房间上调查CGI-100-击倒的K562房间的特征和CGI-100RNA干扰(RNAi)的效果。指向CGI-100基因和与一个不同序列包含一样的核苷酸作文的一双否定控制的三oligonucleotides被设计并且化学上综合了的方法。由在K562房间的shRNA-CGI-100的CGI-100表示的抑制效率用semiquantitativeRT-PCR和点污点杂交被决定。K562房间的生长上的CGI-100RNAi的效果用MTT试金被检验，房间区别被不同途径包括流动cytometry，benzidine染色和电子显微镜测量。在CGI-100-konckdownK562细胞为48h与matrine的0.2mg/ml或hemin的30mol/L被孵化以后，GlycophorinA(GPA)(CD235a)和生长因素independence-1BmRNA(Gfi-1BmRNA)的表示层次被RT-PCR和GPA的蛋白质层次测量，CD14和CD15被流动cytometry检测。结果CGI-100RNAi的真核细胞的表示向量成功地被构造。K562/shRNA-CGI-100房间线在由shRNA-CGI-100的CGI-100基因表示的抑制效率在哪个是54%被建立。CGI-100-knockdown禁止了增长并且在K562房间导致了erythroid区别。与控制K562房间相比，K562/shRNA-CGI-100房间证明减少的吸收度价值由MTT试金，减少的enchromation，增加的heterochromation，G0/G1阶段房间的增加的百分比，S阶段房间的减少的人口，减少的PI(房间的增长索引)，和benzidine积极的房间的提高的百分比检测了。而且，到matrine或hemin的K562/shRNA-CGI-100房间的敏感被提高，到matrine的这些房间的敏感对hemin比那高。与控制K562房间相比，在K562/shRNA-CGI-100房间的matrine处理导致了增长，benzidine积极的房间的提高的百分比，GPA和Gfi-1B的显然起来调整的mRNA表达式，和GPA的增加的吝啬的荧光紧张(MFI)的增加的禁止的率。没有CD14表示被检测，没有统计意义被作出对有利的裁决检测CD15。最后，在与hemin对待并�

简介：Objective:Toinvestigatetheinvitrocleavageabilityandeffectsonapoptosisandcellgrowthofthebcr-ablfusiongenespecificmulti-unitribozymes.Methods:Threefusionpointspecificribozymesweredesignedandthemulti-unitribozymes'invitrotranscriptionvectorandretroviralvectorwereconstructed.Theinvitrocleavageabilitywastested.TheretroviralvectorwastransfectedintoK562cellandtheeffectsonproliferation,apoptosis,cellcycleandcellstructurewereobserved.Results:Multi-unitribozymeshadinvitrocleavageefficiencyof70.8%,whichwasmoreefficientthansingle-unitanddouble-unitribozymes.TransfectionoftheretroviralvectoroftheribozymeintoK562cells,inducedinhibitionofcellgrowthandapoptosis.TheincorporationrateofDNAinribozymestransfectedK562cellswasgreatlydecreasedalongwithtimepassed,withaninhibitionrateofmorethan50%after96hoftransfection.UnderFCM,18.4%ofthecellsunderwentapoptosis72haftertransfectionandmorecellswereblockedinGphase,withtheratioinSphasegreatlydecreased(41.9%).Underelectronmicroscope,compactionofnuclearchromatinandapoptosisbodieswereobserved.Conclusion:Multi-unitribozymesspecifictobcr-ablfusiongenecanbeusedtotreatCMLandtopurgebonemarrowforself-grafting.

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简介：ＳＥＲＯＥＰＩＤＥＭＩＯＬＯＧＩＣＡＬＳＴＵＤＩＥＳＯＦＥＰＳＴＥＩＮＢＡＲＲＶＩＲＵＳ（ＥＢＶ）ＩＮＦＥＣＴＩＯＮＢＹＴＥＳＴＩＮＧＡＮＴＩＢＯＤＩＥＳＡＧＡＩＮＳＴＥＢＶＳＰＥＣＩＦＩＣＤＮＡＳＥ（ＥＤＡｂ）ＡＳＡＭＥＴＨＯＤＦＯＲＥＡＲＬＹ...

简介：Palliativeradiotherapy(pRT)isprimarilyemployedforpalliationofbonepaininpatientswithcastrate-resistantprostatecancer(CRPC).However,evidencethatpRTinfluencesprostate-specificantigenresponseinpatientswithCRPConsystemictherapyislacking.WedescribethreecasesofCRPCprogressingaftertreatmentwithdocetaxel(n=2)andabiraterone(n=1),whorespondedunusuallyafterpRTforbonepainwiththedevelopmentofasignificantbiochemicalresponseandrestorationofresponsetosystemictherapy.ThepossibilityofpRTinfluencingmetastaticdiseaseinCRPChasnotbeenpreviouslyreported,andraisesthepossibilityofradiation-inducedmodulationofanti-tumorimmuneresponsemechanismsthatmayplayaroleintherestorationofresponsetosystemictreatment.