简介：ＩＮＳＩＴＵＬＡＢＥＬＩＮＧＡＰＯＰＴＯＳＩＳＩＮＢＲＥＡＳＴＣＡＮＣＥＲＡＳＲＥＬＡＴＥＤＴＯＰＲＯＧＮＯＳＩＳＷｕＪｉｏｎｇ吴炅ＳｈａｏＺｈｉｍｉｎ邵志敏ＪｉａｎｇＭｉｎｇ江明ＨａｎＱｉｘｉａ韩企夏ＺｈａｎｇＴｉｎｇｑｉｕ张廷ＳｈｅｎＺｈｅｎｚ...

简介：Objective:Toinvestigatetheexpressionofimmune-relatedmoleculesinglioblastomamultiform(GBM)cells.Methods:Theexpressionofmajorhistocompatibilitycomplex(MHC),β2-microglobulin,Fas,CD80andCD86moleculesonthesurfaceofGBMcellswereevaluatedbyflowcytometry.TheexpressionofTAP-1,TAP-2andTapasinintheGBMcellswereevaluatedbyRT-PCRmethod.Results:MHCclassⅠ,β2microglobulin,TAP-1,TAP-2andtapasinwereexpressedinmostGBMcelllines.ExceptU87,therewasnoMHCclassⅡmoleculeexpressiononanyoftheotherGBMcelllines.FaswasexpressedonalltheGBMcelllinesexamined.Conclusion:ThemechanismbywhichGBMescapesimmunesurveillancemayinvolvedownregulationofexpressionofMHCclassⅠmoleculesandMHCclassⅡmolecules.MHCclassⅠpositiveGBMmaybethesuitabletargetofimmunotherapy.

简介：Objective:Toclonemultidrugresistance(MDR)relatedgenesinlungadenocarcinomacelllines.Methods:ThedifferentiallyexpressedcDNAfragmentsbetweenA549andA549DDPcellswereanalyzedbymRNAdifferentialdisplayPCR(DDRT-PCR).ThefragmentsthusobtainedwerefurtheranalyzedbyDNAsequencingandNorthernblotting.Results:ThreedifferentiallyexpressedcDNAfragmentswereobtainedandconfirmedbyNorthernblot.Sequenceanalysisrevealedthattwoofthemwerenovelandonewas100%identicalwithICEgene.Conclusion:AnalyzingdifferentiallyexpressedfragmentbetweenA549andA549DDPcellsmaybehelpfulforfindingnewMDRrelatedgenes.ThedrugresistanceofA549DDPcellsmayberelatedtotheinhibitionordown-regulationofICEgene.

简介：调查apoptosis的表示的目的联系了基因p53；在乳房的管的不正常的增生的bcl-2；在基因表示之间的关系；胸的瘤形成。apoptosis的方法mRNA联系了基因p53；bcl-2被原位杂交在不正常的管的增生的44种情况中检测。p53蛋白质表示被免疫组织化学检测。数据与良性的增生的6个案例的那些相比；胸癌的26个案例。结果p53mRNA的表示在良性的增生是66.7%，40%在不正常的管的增生(55.6%在里面温和，41.7%在媒介，26.1%在里面严重)；19.2%在癌(哪个21.4%是管内的癌；16.7%是侵略的)。p53蛋白质的表示在良性的增生是否定的，24%在不正常的增生(温和11.1%，媒介25%，严重34.8%)，38.5%在癌(管内癌35.7%，侵略管的癌41.7%)。bcl-2的表示在良性的增生是否定的，78.6%在管内癌，83.3%在侵略管的癌。结论损失；p53基因的变化；过多的表示bcl-2mRNA在严重不正常的管的增生被检测。

简介：Objective:ToassesstheeffectofantiviraltherapyforhepatitisBvirus(HBV)-relatedhepatocellularcarcinoma(HCC)afterradicalhepatectomy.Methods:Atotalof478HBV-relatedHCCpatientstreatedbyradicalhepatectomywereretrospectivelycollected.Patientsinthetreatmentgroup(n=141)receivedpostoperativelamivudinetreatment(100mg/d),whereaspatientsinthecontrolgroup(n=337)didnot.Recurrence-freesurvival(RFS)rates,overallsurvival(OS)rates,treatmentsforrecurrentHCCandcauseofdeathwerecomparedbetweenthetwogroups.Propensityscorematching(PSM)analysiswasalsoconductedtoreduceconfoundingbiasbetweenthetwogroups.Results:The1-,3-,and5-yearRFSratesdidn’tsignificantlydifferbetweenthetwogroups(P=0.778);however,the1-,3-,and5-yearOSratesinthetreatmentgroupweresignificantlyhigherthanthoseinthecontrolgroup(P=0.002).Similarresultswereobservedinthematcheddata.SubgroupanalysisshowedthatantiviraltreatmentconferredasignificantsurvivalbenefitforBarcelonaClinicalLiverCancerstageA/Bpatients.FollowingHCCrecurrence,morepeopleinthetreatmentgroupwereabletochoosecurativetreatmentsthanthoseinthecontrolgroup(P=0.031).Forcauseofdeath,fewerpeopleinthetreatmentgroupdiedofliverfailurethanthoseinthecontrolgroup(P=0.041).Conclusion:PostoperativeantiviraltherapyincreaseschancesofreceivingcurativetreatmentsforrecurrentHCCandpreventsdeathbecauseofliverfailure,therebysignificantlyprolongingOS,especiallyinearly-orintermedian-stagetumors.

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简介：Monoclonalantibody(MAb)toratlivercyto-chromeP-450jisozyme,anactivatingenzymespecifictonitrosaminemetabolism,wasusedcoupledwithimmunoblotting,densitometerscanningofSDS-PAGEgelsandimmunohistochemicaltechnique.ThetraceP-450HSjisozyme(Mr.51.5Kd)wasfoundinhumangastricmucosa.ItwassimilartoP-450jinmolecularweight,catalyticandimmunochemicalproperties.TheconcentrationsofP-450HSjinmucosaoflessercurvaturewerehigherthanthoseingreatercurvature.Thismightbeoneoftheimportantreasonsthatlessercurvatureisthecommonestareaforgastriccarcinoma.ButtherewaspossiblylessP-450HSjingastricmucosawithcancer.Im-munohistochemically,P-450HSjwasdiscoveredinthecytoplasmofsomeglandularepithelialcells,especiallyintheglandswithhyperplasticandintestinalmetaplasticchangesadjacenttocarcinoma.Itwasalsofoundinsomenormalglandsandintumorcellsofhigh-differentiatedadenocarcinoma,butnotinthoseoflow-differentia

简介：Objective:Toexploretherelationshipbetweenperoxisomeproliferatoractivatedreceptor-gamma(PPARγ)andperoxisomeproliferator-activatedreceptor-gammacoactivator-1(PGC-1)expressioningastriccarcinoma(GC),andanalyzetheircorrelationswithclinicopathologicalfeaturesandclinicaloutcomesofpatients.Methods:Thetwo-stepimmunohistochemicalmethodwasusedtodetecttheexpressionofPPARγandPGC-1in179casesofGC,and108casesofmatchednormalgastricmucosa.Besides,16casesoffreshGCspecimensandcorrespondingnormalgastricmucosaweredetectedforPGC-1expressionwithWesternblotting.Results:ThepositiveratesofPPARγandPGC-1expressionweresignificantlylowerinGC(54.75%,49.16%)thaninnormalgastricmucosa(70.37%,71.30%),respectively(P<0.05).ThedecreasedexpressionofPGC-1inGCwasconfirmedinourWesternblotanalysis(P=0.004).PPARγandPGC-1expressionswererelatedtoLauren’stypesofGC(P<0.05).PositivecorrelationwasfoundbetweenPPARγandPGC-1expressioninGC(rk=0.422,P<0.001).ThesurvivaltimeofPPARγnegativeandpositivepatientswas36.6±3.0vs.38.5±2.7months,andnostatisticaldifferencewasfoundbetweenthe5-yearsurvivalratesoftwogroups(34.4%vs.44.1%,P=0.522,log-ranktest);thesurvivaltimeofPGC-1negativeandpositivepatientswas36.2±2.8vs.39.9±2.9months,whilenostatisticaldifferencewasfoundbetweenthe5-yearsurvivalratesofthetwogroups(32.0%vs.48.2%,P=0.462,log-ranktest)Conclusions:DecreasedexpressionofPPARγandPGC-1inGCwasrelatedtotheLauren’sclassification.TheirexpressionsinGCwerepositivelycorrelated,indicatingthattheirfunctionsingastriccarcinogenesismaybecloselyrelated.

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简介：Objective:ToanalyzethedifferentiallyexpressedcDNAsequencesrelatedtochlorophyllin(CHL)mediatedinhibitionofmalignanttransformationofhumanbronchia1epithelialcellline(16HBE).Methods:16HBEcellstreatedwithchlorophyllinandanti-BPDEwereconductedastester,16HBEcellstreatedonlywithanti-BPDEwereconductedasdriver,andcDNArepresentationaldifferenceanalysis(cDNARDA)wasusedtocomparethedifferentialgeneexpressionbetweenthetwokindsofcells.ThecDNAfragmentswereligatedtopGEM-TvectorandtransformedintoJM109bacteria.TheplasmidDNAwassequencedandcomparedwithdatabaseinGenBankbyBLASTN.Results:Amongthe5clonedcDNAsequences,threewerenovelandwereregisteredindbESTdatabase,twoshowedsequencehomologytoalpha-enolaseandanewlyfoundgeneribosomalproteinS18/S6-like.Conclusion:These5cDNAsequencesmightplayimportantrolesinantitransformingeffectofchlorophyllin.

简介：Objective:Theaimsofthiscross-sectionaldescriptivestudyweretoevaluatethequalityoflife(QoL)ofthelungcancerpatientsandtoinvestigatedifferencesinQoLwithrespecttogeneralandmedicalcharacteristics.Methods:Structuredquestionnaires(EORTCQLQ-C30andQLQ-LC13)wereusedamong106consecutivelungcancerpatientsfordatacollectionduring1Jan2002to31Dec2002.Thet-testandone-wayanalysisofvariance(ANOVA)wereusedtocomparedifferencesofQoLbetweenthefactorsata5%levelofsignificance.Results:Thestudyrevealedthatthequalityoflifeofthelungcancerpatientswereworsethanreferencevalue.Theyoung,maleandmarriedpatientgroupshadbetterQoL.PatientswithlowereducationorincomehadworseQoL.SmallcelllungcancerpatientsreportedpoorerQoLthannon-smallcelllungcancerpatients.ThequalityoflifeinpatientsatlatestageorwithmetastasishadworseQoL.Thetreatmentscouldworsenthequalityoflife.Whentheoutcomesofthefourtreatmentswerecompared,thesurgerygroupdisplayedthebestqualityoflifeandthecombinedtreatmentgroupdisplayedtheworstqualityoflife.Conclusion:Theresultsofthepresentstudyshowedimportantramificationsforclinicians,researchersandpolicy-makers.

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