Toevaluatetheeffectofadenovirus-mediatedp53gene(Adp53)onapoptosisandradiosensitivityofhumangastriccarcinomacelllines.Methods:Recombinantadenovirusexpressingwild-typep53lineswithdifferentp53geneticstatus.p53proteinexpressionwasdetectedbyimmunohistochemistryassayandwesternblotassay.Cellsurvivalwasassessedusingaclonogenicassay.TUNELassaywasusedindeterminationofapoptosis.FourhumangastriccarcinomacellsinfectedwithAdp53wereirradiatedwith4GyandcellcycledistributionandSub-G1peakwereassayedbyflowcytometry.Results:G2/Marrest,apoptosisandinhibitionoftumorcellproliferationwereinducedbyinfectionatAdp53at100MOIwhichcausedhightransferrateofwild-typep53andstrongexpressionofp53proteininfourhumangastriccarcinomacells.Theradio-enhancementratioofAdp53at4Gywere3.0forWcell,3.6forMcell,2.2forneocelland2.5for823cellinvitro.Conclusion:ThisstudydemonstratedthatAdp53transferincreasedcellularapoptosisandradiosensitivityofhumangastriccarcinomacelllinesinvitroindependentlyoncellularintrinsicp53statusthussupportingthecombinationofp53genetherapywithradiotherapyinclinicaltrials.
