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简介：ＡＣＬＩＮＩＣＡＬＲＥＰＯＲＴＯＦＲＥＦＲＡＣＴＯＴＹＣＡＲＣＩＮＯＭＡＯＦＯＶＡＲＹＡＮＤＦＡＬＬＯＰＩＡＮＴＵＢＥＴＲＥＡＴＥＤＷＩＴＨＴＡＸＯＬＣａｉＳｈｕｍｏ蔡树模；ＴａｎｇＪｉｅ汤洁；ＦａｎＪｉａｎｘｕａｎ范建玄；ＷｅｎｇＺｈｏｎｇｙｉｎｇ...

简介：客观：在人的hepatocellular癌(HCC)在HLA医生表示上调查干扰素(IFN)的管理效果。方法：房间衬里的在4种HCC的HLA医生的表示，HHCC，SMMC-7721，BEL-7402，HCC-9204和一个正常的肝房间衬里QZG在被IFN-或IFN-的不同剂量刺激前后与immunohistochemicalABC和ELISA方法被检测。结果：仅仅SMMC-7721房间的小部分表示了另外的房间线的HLA医生，和所有是在有IFN-或IFN-的刺激前否定的HLA医生。HLA医生表示在刺激以后5根房间线在所有被提高，并且它与IFN的剂量被相关。QZG房间不比HCC房间明显。IFN-的效果比IFN-的更明显。结论：IFN能在HCC房间提高HLA医生表示。

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简介：SupraglotticlaryngectomywasfirstreportedbyAlousein1974andacceptedgradually.{’lAtventricularlevelincludingventricleband,vestibule,aryepiglotticfold,epiglottis,preepiglotticspaceandhyoidboneexcisionwascompleted.Itwasalsocalledhorizontalsupra-hemilaryng...

简介：AbstractObjective:To investigate the expressions of MAPK10, c-Jun and Itga6 in laryngeal carcinoma and its influence on the sensitivity to docetaxel, cisplatin and 5-fluorouracil (TPF) chemotherapy.Methods:Fifty-seven patients with supraglottic squamous cell carcinoma, who were treated by two cycles of TPF induction chemotherapy in our hospital, were enrolled in this study and divided into groups by chemotherapy resistance or chemotherapy sensitivity. The expressions of mRNA and protein of MAPK10, c-Jun and Itga6 in tumor tissues were evaluated by immunohistochemistry. The consistency of mRNA and protein expressions was tested, and the relation with the clinicopathological features was analyzed.Results:The positive rates of MAPK10 andc-Jun in the tumor tissues of the sensitive group were significantly higher than those of there assistant group, which was 90.48% and 100.00%, respectively. The expression rate of Itga6 was significantly higher in the resistant group, which was 83.33% (P < 0.05). The mRNA levels of MAPK10 and c-Jun were significantly lower in the resistant group than in the sensitive group, whilethemRNA levelof Itga6was significantly higher in the resistant group (P < 0.05). The protein expressions of MAPK10, c-Jun and Itga6 were consistent with their mRNA expressions (P < 0.05). The expressions of MAPK10, c-Jun and Itga6 were not correlatedwithage, gender and tumor diameter (P > 0.05). However, the expressions of MAPK10 and c-Jun were negatively correlated withclinical stage and pathological grading (P < 0.05). Negative correlations between MAPK 10 and Itga6, and between c-Jun and Itga6in tumor tissues were found by Spearman’srank correlation coefficient (P < 0.05). The correlation was also negative in the resistant tumor tissues (P < 0.05).Conclusion:The MAPK10 and c-Jun expressions were down-regulated, while the Itga6 expression was up-regulated in the chemo-resistant laryngeal carcinoma, and the expression levels of different factors were correlated witheach other. These factorsmight be important biomarkers for predicting outcomes of TPF chemotherapy in laryngeal carcinoma in the future.

简介：Objective:Tostudytheexpressionofvascularendothelialgrowthfactor(VEGF)andmicrovesseldensity(MVD)inesophagealsquamouscellcarcinoma(ESCC)andclarifytheassociationofVEGFexpressionwiththeangiogenesisandprognosticvalueofthisdisease.Methods:Eighty-twocaseswithprimaryESCCtreatedwithradicaloperationinDepartmentofSurgeryfromJan1981throughMay1994wereenrolled.VEGFexpressionandMVDvaluewereexaminedbyimmunohistochemicalstaining,thestreptavidin-biotinperoxidasecomplexmethod(SPmethod),usinganti-VEGFpolyclonalantibodyandanti-Factor-VIIIantibody,respectively.WealsoanalyzedtherelationshipbetweenVEGFexpressionandMVDvalueandpostoperativesurvivalrateofpatients.Results:Ofthe82cases,63.4%casesshowedpositiveforVEGFintumorcellsandthemedianofMVDintumorwas37(9-150)·mm-2.TherewasaclosecorrelationbetweenMVDandVEGF(P=0.001).The5-yearsurvivalrateofpatientswithlowandhighMVDwas34.1%and12.2%,respectively.The5-yearsurvivalratewas46.7%inpatientswithVEGF-negativetumorand11.5%inpatientswithVEGF-positivetumor.Thesedifferenceswerestatisticallysignificant(P=0.017andP<0.001,respectively).Conclusion:InESCC,angiogenesisismediatedmainlybyVEGFandVEGFmaybeassociatedwithtumorprogressionandincreasedmalignancyviaangiogenesis.

简介：Thymidinephosphorylase(TP)isakeyenzymethatcontributestothecompositionanddecompositionofpyrimidinenucleotides.TPseemshomologoustoplatelet-derivedendothelialcellgrowthfactor,anditseffectsoninducingvascularizationandanti-apoptosisarecloselyrelatedtogrowthandmetastasisofcolorectalcarcinoma.Inaddition,TPisakeyenzymethatcatalyzesthetransformationfrom5-fluorouracil(FU)prodrugsof5′-deoxy-5-fluorouridine(5′-DFUR)to5-FU.TheactivityofTPiscloselyrelatedtothesensitivityofcolorectalcarcinomacellstofluorouracildrugsandtargetedtherapy.GiventheimportantfunctionsofTPingrowth,metastasis,tumortreatment,andprognosis,determiningitsexpressionmechanismissignificant.ThisarticlesummarizestheresearchdevelopmentofTPexpressionincolorectalcarcinoma,tumorneovascularization,cytotoxicityactivationof5′-DFUR,andcolorectalcarcinomatherapy.

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简介：Objective:Secretorybreastcarcinoma(SBC)isararetypeofbreastmalignancy,accountingforlessthan0.02%ofallinfiltratingbreastmalignancies.ThepureSBC,atypeofSBCwithoutanothertypeofbreastmalignantneoplasm,isparticularlyrare.ThisstudyaimedtoinvestigatetheclinicopathologicandmolecularfeaturesofpureSBC.Methods:Themainpathologicalparameterssuchasestrogenreceptor(ER),progesteronereceptor(PR),andhumanepithelialgrowthfactorreceptor2(C-erbB-2)weredetectedbyimmunohistochemistry(IHC),andtheclinicopathologicandprognosticdifferencewerecomparedwithinvasiveductalcarcinoma(IDC).Fluorescentinsituhybridization(FISH)andreversetranscriptionpolymerasechainreaction(RT-PCR)wasperformedtoidentifytheETV6-NTRK3rearrangementofSBC.Results:WefoundthatthepositivityratesofER,PR,C-erbB-2,p53,andS-100were47.7%(21/44),52.3%(23/44),36.4%(16/44),27.3%(12/44),and95.5%(42/44),respectively,whichwerehigherthanthosereportedinpreviousstudies.Specialperiodicacid-Schiffanalysiswasperformedin36patients,andthevalueoftheKi-67indexrangedfrom1%to50%(meanvalue:10%).Interestingly,mostpatientswithpureSBCharboredanETV6-NTRK3rearrangementwithan88.6%(39/44)expressionrate.ComparedwithIDC,thetumorsizeofmostpatientswithSBCwaslargerthan2cm(P=0.024).Ultrasoundshowedbenignlesions,andthetotalmisdiagnosisratewashigher(P=0.020).Althoughthepathologicalclassificationwasmostlytriple-negativebreastcancers(P=0.036),therewaslessmetastasis(P=0.029),andtheoverallprognosiswasbetterthanthatoftheIDCgroup.Conclusions:Althoughaxillarylymphnodemetastasis,localrecurrence,ordistantmetastasismayoccur,SBCisalsoconsideredanindolentneoplasmwithagoodprognosis.Oncediagnosed,surgicaltreatmentshouldbeperformedassoonaspossible,followedbyappropriateadjuvantchemotherapy,irradiation,andendocrinetherapies.

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简介：摘要：在中介或先进的胰腺的头癌上改进辩解的效果。方法：手术与intermediated或先进的胰腺的头癌在26个病人被动。在癌上与一根电子横梁与intraoperative放射疗法相结合的Cholecystojejunostomy或choledochojejunostomy从1996年5月被执行到1998年5月。同时，多功能的可植入的药交货系统的导管为手术后的灌注化疗经由胃与十二指肠的动脉被插入。结果：327月后续调查建议肿瘤在治疗的功课以后在不同的度缩小了。所有病人疼痛被减轻。6月、12月、24月的幸存率是100%，93.9%和20%分别地。5个死了的病人的平均幸存时间是17.8个月。结论：这操作是很完成与中介或先进的胰腺的头癌延长病人的生活。

简介：ＴＨＥＤＩＡＧＮＯＳＩＳＡＮＤＴＲＥＡＴＭＥＮＴＦＯＲＲＥＣＵＲＲＥＮＴＤＹＳＰＨＡＧＩＡＯＦＥＳＯＰＨＡＧＥＡＬＣＡＲＣＩＮＯＭＡＡＦＴＥＲＲＡＤＩＣＡＬＲＡＤＩＯＴＨＥＲＡＰＹＣｈｅｎＫｅｎｅｎｇ陈克能ＣｈｅｎｇＢａｎｇｃｈａｎｇ程邦昌Ｄｅｐａ...

简介：瞄准：在食道的有鳞的房间癌(ESCC)调查midkine的表示并且与clinicopathological特征分析它的关系。方法：RT-PCR和免疫细胞化学的染色被用来分别地在EC109房间检测midkinemRNA和蛋白质的表示。然后，在ESCC样品的66种情况中的midkine的表示被免疫组织化学对人的midkine用单音的同种细胞的抗体检测。结果：Midkine被RT-PCR和免疫细胞化学在EC109房间表示。免疫反应在56.1%被检测(37/66)ESCC样品。midkine的表示在肿瘤房间的细胞质被发现。尤其是，midkine的紧张在充满容器和肿瘤的入侵的边阶的区域是更强壮的。Midkine更强烈地比在中等并且糟糕区分的肿瘤在很好区分的肿瘤(76.9%)被表示(43.1%和41.2%，分别地)(P<0.05)。在midkine之间没有统计上重要的关联在ESCC的表示和性，年龄，临床的阶段，淋巴节点转移或幸存。结论：Midkine完了在ESCC表示了。它可以在肿瘤血管生成和侵略起一个作用。midkine的表示在ESCC与肿瘤细胞分化被相关。肿瘤房间区分越多糟糕，midkine表示越多weaker。

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简介：Objective:Tostudytheexpressionofdendriticcellsinhumanrenalcellcarcinomaandexplorethecause,sotorevealthemechanismofescapingimmunesurveillanceinRCC.Methods:TheexpressionsofCD83+DCS,CD1a+DCS,VEGFandTGF-β1intumoral,peritumoralandnormalkidneytissuesofRCCin30casesweredetectedbyimmunohistochemistryusingstreptavidin/peroxidese(SP)Results:CD83+DCSweremainlylocatedintheperitumoralareas;whereasCD1a+DCS、weremainlyretainedwithinthecancernests.ThenumberofCD83+DCSwasinverselycorrelatedwiththeclinicalstage(P<0.05);buttherewerenosignificantcorrelationsbetweenthenumberofCD1a+DCS、andtheclinicalstage(P>0.05).TheexpressionsofCD83+DCSandCD1a+DCShavesignificantdifferencebetweenthetumoral,peritumoralandnormalkidneytissues(P<0.001).TheexpressionofVEGFandTGF-β1weresignificantlylowerinsampleswithhighlyinfiltratingCD83+DCS(P<0.05);WhereasCD1a+DCSwerenot(P>0.05).Conclusion:DChasthetendencytogatheringintumor,butbecauseoftheimmunosuppressivecytokins,forexampleVEGFandTGF-β1,inhibitsthematurationofDC,therearelessmatureTIDCS(CD83+TIDCS)inthetumoraltissues,theyaremainlylocatedintheperitumoralareas.ThismaycontributetothemechanismofescapingimmunesurveillanceinRCC.

简介：Objective:IL-22-producingCD4+Thelpercells(Th22cells)havebeenidentifiedasmajorinducersoftissueinflammationandimmuneresponses.Currently,nopreviousstudyexploredtheroleofTh22cellsinthepathogenesisofhepatocellularcarcinoma(HCC).ThestudyaimedtodeterminethebiologicalfunctionofTh22cellsanditseffectorIL-22inHCCpatients.Methods:Forty-fiveHCCpatientsand19healthycontrolswererecruitedandtheirperipheralbloodwascollected.ThefreshHCCtissues,adjacentHCCtissuesandtennormallivertissueswerealsocollected.FlowcytometryanalysiswasusedtodeterminethefrequenciesofcirculatingTh22cellsandTh17cells.SerumIL-22levelsweretestedbyenzyme-linkedimmunosorbentassay(ELISA).Immunohistochemicalstainingandreal-timepolymerasechainreaction(PCR)wereusedtodetectIL-22proteinandmRNAintissuesspecimens,respectively.Results:CirculatingTh22cells,Th17cellsandserumIL-22levelsweresignificantlyelevatedinHCCpatientscomparedwiththoseofhealthycontrols(P<0.001).Th22cellswereshowedtobepositivelycorrelatedwithIL-22inHCCpatients(P<0.05),butnotinhealthycontrols.NosignificantdifferenceswerefoundinHCCpatientswithHBeAgpositivityornegativityintermofTh22cellsandserumIL-22levels.TheexpressionofIL-22proteinandmRNAwashighestinHCCtissues,followedbyadjacentHCCtissuesandnormallivertissues.Furthermore,Th22cells,serumIL-22levelsandIL-22mRNAwereelevatedatstageIII-IVcomparedwithstageI-IIofHCC(P<0.05).Conclusions:ElevationofcirculatingTh22cellsandIL-22maybeimplicatedinthepathogenesisofHCC,andpotentiallybecellulartargetsfortherapeuticintervention.