简介:AIM:ToinvestigatetheeffectofY-27632onthesurvivalandneuriteoutgrowthoftheculturedretinalneurocytes.METHODS:Afterthepostnatalday2-3,Sprague-Dawleyretinalneurocyteswereculturedfor48hours,theculturemediawasreplacedwithserum-freemedia(controlgroup)andserum-freemediacontained30μmol/LY-27632(Y-27632group),andthecellswerecontinuallyculturedanother48hours.Theculturedretinalneurocyteswereidentifiedwithanti-neuronspecificenolase(NSE)immunocytochemistry.ThesurvivalstateofthosecellswasestimatedbyMTTassay,andtheneuriteoutgrowthofthosecellswasevaluatedbythecomputerizedimage-analysissystem.RESULTS:Comparedwiththecontrolgroup,theabsorbancevaluesofcellssurvivalinY-27632groupincreased12.90%and33.33%respectivelyafter72and96hoursculture.Y-27632hadnosignificanteffectonthediameterofculturedretinalneurocytes.Comparedwiththecontrolgroup,Y-27632inducedastableimprovementofneuriteoutgrowthofretinalneurocytesafter72and96hoursculture(P=0.001).CONCLUSION:Y-27632couldpromotethesurvivalandneuriteoutgrowthoftheearlypostnatalculturedretinalneurocytes.
简介:AIM:ToinvestigatetheregulationofEaf2proteininmouselenscellsapoptosisinducedbyultraviolet(UV)radiation.METHODS:AneyeofEaf2geneknockoutmiceornormalcontrolmicewasexposedtoUVradiation,andtheotheronewasnon-exposed.AlloflenseswereanalyzedbyTUNELandcaspase3activityassaystodeterminethedifferenceoftheapoptosisinducedbyUVradiation.Inaddition,exposedandnon-exposedlenseswereanalyzedbyquantifiedp53expressionandreal-timereversetranscription-polymerasechainreaction(RT-PCR)ofBax,Bid,Apaf-1,PumaandNoxa,tocompareEaf2geneknockoutmiceandnormalcontrolmice.RESULTS:UVradiationcausedapoptosisoflenscellsinnormalcontrolmiceandEaf2knockoutmice.Activityofcaspase3wassignificantlyhigherinnormalcontrolmicethanEaf2knockoutmice.Expressionofp53proteinwassignificantlyhigherinlensesexposedtoUVradiationthannonexposedlenses,butwassimilarbetweenEaf2geneknockoutmiceandnormalcontrolmiceinthesameUVcondition.AfterexposingtoUVradiation,theanalysisofreal-timeRT-PCRdemonstratedthatmRNAlevelsofPumaandNoxaweresignificantlyhigherinlensesofnormalcontrolmicethanEaf2geneknockoutmice,andthatmRNAlevelsofBax,BidandApaf-1werenotsignificantlydifferentbetweengeneknockoutmiceandnormalcontrolmice.CONCLUSION:Eaf2increaseslenscellsapoptosisinducedbyultravioletradiation.AndEaf2up-regulatesexpressionofthePumaandtheNoxatoactonlenscellsapoptosisafterUVradiation.
简介:目的探讨应用颅锥细孔钻颅置管低位持续引流治疗慢性硬膜下血肿的临床疗效.方法回顾并分析40例临床病例资料:其中20例为对照组,即常规钻孔引流组:常规于顶结节下方钻颅,置8号导尿管于血肿腔内,用生理盐水反复冲洗,外接无菌瓶,术后2~3日拨管.另20例为颅锥细孔钻颅治疗组:顶结节下方颅锥钻颅,置入细硅胶管,深入颅内3cm左右,管全长15cm,内径1.5mm,外径2.4mm,管前端为盲端,其后0.5~1.0cm处有四个小侧孔.本组10例用生理盐水及庆大霉素盐水反复冲洗,另10例不予冲洗,均接无菌瓶,悬挂于钻孔点下方30~50cm处持续引流,1~2日拨管,创口不予缝合,加压包扎.结果术后48h头颅CT扫描显示:颅锥细孔钻颅治疗组20例患者无颅内积气,硬膜下积液较少,脑中线结构基本复位,术后临床证状迅速改善,无感染发生,住院时间短,冲洗组与非冲洗组之间相比差异无显著意义,此组临床疗效明显优于对照组.术后50天复查头颅CT所有患者均临床治愈.结论应用颅锥细孔钻颅持续低位引流治疗慢性硬膜下血肿,具有创伤小,头部伤口不需缝合,患者痛苦小,住院费用低、时间短,临床症状改善迅速等优点,尤其适用于广大基层医院推广应用.亦可应用于床边治疗慢性硬膜下血肿.但需注意钻孔位置、引流充分、无菌瓶位置等.
简介:目的对钻颅血肿碎吸术治疗脑出血护理干预价值进行分析。方法本次将2017年1月~2018年12月在我院进行治疗的脑出血患者200例,根据患者入院治疗的先后顺序将其分为两组,干预组(n=100例)和对照组(n=100例),在治疗期间给予对照组患者常规的护理干预,干预组患者实施具有针对性的综合护理干预。对两组患者的护理有效率进行观察比对。结果干预组护理总有效率93%与对照组患者的护理总有效率78%相比较来说显著较高,P<0.05。结论给予实施钻颅血肿碎吸术的患者综合性的护理干预可有效的提高治疗效果,促进患者恢复,因此,值得在临床上进行应用和推广。
简介:摘要 : 目的 : 探讨涡轮钻法拔除下颌阻生第三磨牙的临床效果。 方法 : 90 例行下颌阻生第三磨牙拔除患者 , 根据随机抽号方法分为对照组和实验组 , 各