简介：Smoothened(SMO)是表明小径的刺猬的一个重要成员。我们为RNA干扰构造了特定的recombinantlentiviral向量，指向SMO基因(NM_005631)观察它在人的雄激素敏感的前列腺癌症房间线的SMO表示，房间增长和房间周期上的效果，LNCaP，并且在雄激素无关的前列腺癌症房间线，PC3。四个siRNA序列被设计并且插入了到lentiviral向量pGCSIL-GFP构造四recombinant向量。有最高的介入效率的向量是有在293T房间包装向量(pHelper1.0和pHelper2.0)为分别地感染LNCaP和PC3房间线由liposome装配lentivirus粒子的co-transfected。SMOmRNA，肿瘤房间增长和房间周期的表示水平被量的实时聚合酶链反应(qRT-PCR)测量，3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)试金和流动cytometry分别地。顺序结果证明recombinantlentiviral向量成功地被构造。pGCSIL-GFP-723有最高的介入效率，在co-transfection，LNCaP和PC3房间与排队以后的命名Lv-SIL-SMO723被感染。与控制组相比，显著地显示出的结果减少了(P<0.05)LNCaP和PC3，S阶段房间的更低的吝啬的百分比和G2/M的更高吝啬的百分比的SMOmRNA表情分阶段执行房间，以及显然减缓增长(P<0.01)在感染的组的LNCaP。然而，PC3的增长没被改变(P>0.05)。在结论，recombinantlentivirus粒子能压制SMO表示，在LNCaP和PC3房间线调整房间周期并且显著地禁止LNCaP房间然而并非PC3房间的增长。

简介：AbstractIntroduction:Merkel cell carcinoma (MCC) is a rare, aggressive cutaneous malignancy, and its pathogenesis might relate to ultraviolet light and Merkel cell polyomavirus infection. MCC in the Chinese population is uncommon.Here, we present a case of MCC that occurred based on widespread actinic keratosis (AK) in a Chinese female.Case report:An 82-year-old woman presented with two rapidly enlarging and rupture lesions on the face for 1 year. Biopsy was suggestive of squamous cell carcinoma (SCC) on the forehead and MCC on the left cheek. The patient had a history of generalized AK for 3 years. The lesion on the left cheek was also revealed as an AK by histopathological examination 1 year ago. Complete surgical resection was performed to remove the two malignancies.Discussion:The co-occurrence of AK, SCC, and MCC in a Chinese woman is unusual. Immunohistopathological examination is vital for correct diagnosis. The three tumors, in this case, may originate from two different precursor cells and are affected by the same carcinogen. Alternatively, they may come from the same pluripotent epidermal stem cells, and chronic exposure to ultraviolet light and Merkel cell polyomavirus lead to the formation of different types of tumors. The coexist of MCC with other cutaneous tumors provided a train of thought for exploring the origin of MCC.Conclusion:We reported a rare co-existence phenomenon of MCC associated with AK and SCC. Hence, long-term follow-up and early treatment are imperative for patients with premalignant lesions, such as widespread AK.

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简介：InOctober2003,theThirdPlenarySessionoftheSixteenthCentralCommitteeoftheCommunistPartyofChina(CPC)calledforadherenceto'theoutlooktowarddevelopmentbasedonthefundamentalinterestsofthepeople,towardanall-round,balancedandsustainabledevelopment.'Thesessionalso

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简介：在英语口语中，不同场合一个词汇有许多种用法，如多义词语“Allright”，一些英汉词典将其解释为“好、行、对、同意、没关系”等意思，其实它也可以表示“安全”、“的确”、“正是”等意思。这些意思都是说明说话者的肯定态度的。有时它也可以用于强调、转折和讽刺的场合，表示说话者的恼怒、连接或否定等的不同语气。

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简介：摘要Ma10等人2003年和2005年先后提出了下颌和上颌牙列缺失“All-on-four”种植即刻修复的理念，即无牙单颌植入4枚种植体颌骨前部垂直植入两枚种植体，远中倾斜植入两枚种植体，利用4枚种植体实现种植即刻固定修复。

简介：Weexperimentallydemonstrateamplitudesqueezedsolitonutilizingintensity-dependentself-phasemod-ulationinanasymmetricSagnacinterferometer.Thesystem,whosecomponentsareconnectedviaferruleconnector/physicalconnection(FC/PC)fiberconnectors,constitutesall-fiberconfigurationtogeneratesqueezedsoliton.Solitonamplitudereductionmeasuredbyhomodynedetectionisnear4.0dBbelowtheshot-noiselevel.Optimalsqueezingfieldsinbothsimpleandcompactall-fiberconfigurationareobtained.

简介：In1951ProfessorWeiminZhengreturnedtothePeople'sRepublicofChinafromtheUnitedKingdomtoassistinbuildinghiscountryintoamodernnation.RatherthanpursueadoctoraldegreeattheCaliforniaInstituteofTechnology,orCaltechasitispopularlycalled,ProfessorZheng,unselfishlyandpatriotically,decidedthathewoulddevotehismanytalentstoprovidingafirst-classeducationtoChinesestudentswithinastronguniversitysystem.Inparticular,he

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简介：Theintroductionofdesigneroestrogensasatreatmentmedalityinhormonereplacementinwomenhasinvitedtoconsidertheconceptofcompoundswithselectiveandrogeniceffectsformalehonnonereplacementtherapy.Thefullspectrumoftheactionsoftestosteronemaynotbenecessaryofevenundesiredforcertainindicationsfortestosteronetreatment,Todefineforwhatindicationscertainandrogenicpropertiesaredesiredandundesiredmoreinsightinbasicandrogen(patho)physiologyisrequired.Thereisconvincingevidencethataromatizationofandrogeniccompoundstonestrogensmightbeanadvantageformaintenanceofbonemassanditmightalsomitigatenegativeeffectsofandrogensonbichemicalparametersofcardiovascularrisks:thepotentiallynegativeeffectsofoestmgensonprostatepathologyinageingmenneedsfurtherelucidation.Whiletheroleofdihydro-testosterone(DHT)forthemalesexualdifferentiationandforpubertalsexualmaturationisevident,itsroleinmatureandageingmalesseemslesssignificantormayevenbeharmful.Itis,however,ofnotethatanegativeeffectofDHTonprostatepathophysiolog~iscertainlynotproven.Formalecontraceptionaprogestationalagentwithstrongandrogenicpropertiesmightbeanasset.Formostoftheandrogenicactionsthecriticallevelsofandrogensarenotwellestablished.Thelatterisrelevantsincethelargeamountofandrogenmoleculesrequiredforitsbiologicalactions(ascomparedtooestrogens)isanimpedimentinandrogenreplacementmedalities.Theremayberoomformorebiopotentandrogenssincedeliveryoflargeamountsofandrogenmoleculestothecirculationposesproblemsfurtreatmentmodalities.(AsianJAndro11999Jun;1:21-28)

简介：Polarizationmanipulationisessentialindevelopingcutting-edgephotonicdevicesrangingfromopticalcommunicationdisplaystosolarenergyharvesting.Mostpreviousworksforefficientpolarizationcontrolcannotavoidutilizingmetalliccomponentsthatinevitablysufferfromlargeohmiclossandthuslowoperationalefficiency.ReplacingmetalliccomponentswithMieresonance-baseddielectricresonatorswilllargelysuppresstheohmiclosstowardhigh-efficiencymetamaterialdevices.Here,weproposeanefficientapproachforbroadband,highqualitypolarizationrotationoperatingintransmissionmodewithall-dielectricmetamaterialsintheterahertzregime.Byseparatingtheorthogonalpolarizationcomponentsinspace,weobtainrotatedoutputwaveswithaconversionefficiencyof67.5%.Theproposedpolarizationmanipulationstrategyshowsimpressiverobustnessandflexibilityindesigningmetadevicesofbothlinear-andcircular-polarizationincidences.

简介：Findingthecommonsubstructuressharedbytwoproteinsisconsideredasoneofthecentralissuesincomputationalbiologybecauseofitsusefulnessinunderstandingthestructure-functionrelationshipandapplicationindrugandvaccinedesign.Inthispaper,weproposeanovelalgorithmcalledFAMCS(FindingAllMaximalCommonSubstructures)forthecommonsubstructureidentificationproblem.Ourmethodworksinitiallyattheproteinsecondarystructuralelement(SSE)levelandstartswiththeidentificationofallstructurallysimilarSSEpairs.TheseSSEpairsarethenmergedintosetsusingamodifiedApriorialgorithm,whichwilltestthesimilarityofvarioussetsofSSEpairsincrementallyuntilallthemaximalsetsofSSEpairsthatdeemedtobesimilararefound.Themaximalcommonsubstructuresofthetwoproteinswillbeformedfromthesemaximalsets.ArefinementalgorithmisalsoproposedtofinetunethealignmentfromtheSSEleveltotheresiduelevel.ComparisonofFAMCSwithothermethodsonvariousproteinsshowsthatFAMCScanaddressallfourrequirementsandinferinterestingbiologicaldiscoveries.

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