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简介：Objective:ToevaluatetheclinicalapplicationofmultiplexPCRinthedetectionofTreponemapallidum,Herpessimplexvirus(HSV),andHaemophilusducreyi.Method:ThreestandardstrainswereusedtosetupamultiplexPCR(MPCR)fordetectingsyphilis,herpesgenitalis,andchancroidsimultaneously.Samplesfrom122patientswithgenitalulcerdisease(GUD)weresubjectedtoMPCRandtheresultswerecomparedwiththeseofdark-fiddmicroscopyandTPserology,HSVanligenELISA,andH.ducreyiculture,Result:Inthe122patientswithGUD,MPCRidentified34casesofT.palliduminfection,40casesofHSVinfection,and2casesofmixedinfectionofT.pallidumandherpes.NopositiveresultsofH.ducreyiwerefound.ThesensitivityofMPCRtoT.pallidumandherpeswas100%and93.3%,respectivdy.Thesensitivitiesofdark-fieldmicroscopyandTPserology,HSVantigenELISA,andH.ducreyiculturewas35.3%,50%and100%,respectively.Conclusion:MPCRshowedarelativelyhighersensitivityforT.pallidumascomparedwiththeroutinetechniques.AlthoughitssensitivityforHSVwasnotasgoodasthatofantigenELISA,italsoyieldedahighdetectionrate.MPCRcandetectmorethanonepathogen.Itissimple,quick,sensitive,andsuitableforclinicaluseorepidemiologicalinvestigation.

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