简介：Objective:Todeterminetheroleofhemoglobin(HB)-inducedhemeoxygenase-1(HO-1)ininjuredlungscausedbylimbischemia-reperfusion(I/R)inrats.Methods:Aratmodelofischemiainthehindlimbswasmadebyclampingtheinfrarenalaortawithamicrovascularclip,andlunginjuryoccurredafterreperfusion.ToinducetheexpressionofHO-1inthelungs,Hbwasadministratedintraperitoneallyat16hoursbeforereperfusion.NorthernblottingandWesternblottingwereusedtodetecttheexpressionofHO-1inthelungs,andthecarboxyhemoglobin(COHb)levelinarterialbloodwasassayed.Theeffectofhemoglobin(Hb)ontheinjuredlungsafterlimbI/Rwasdeterminedbymeasuringthechangesoflunghistology,polymorphonuclear(PMN)count,malondialdehyde(MDA)contentandwet-to-dryweightratio(W/D).Zincprotoporphyrin(ZnPP),aninhibitorofHO,wasusedtodeterminewhetherHO-1wasinducedbyHbafterlunginjury.Results:HbledtoasignificantincreaseinHO-1mRNAandproteinexpressioninthelungs,accompaniedbytheincreaseofCOHblevelinarterialblood.Comparedwiththeshamcontrols,thelungPMNcount,MDAcontentandW/Dsignificantlyincreasedat4hoursafterlimbI/R,whichreversedbythepretreatmentwithHbat16hoursbeforereperfusion.ZnPPblockedthisprotectiveroleofHbintheinjuredlungs.Conclusions:HbcaninducethelungHO-1expression,whichplaysanimportantroleinthedefenseagainstI/R-inducedlunginjuryinrats.
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