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简介：Treated50casesofrenalcolicwithelectroacupunctureandcomparedtheresultswiththoseintwocontrolgroups.Totaleffectiverateinthetreatmentgroup,controlgroupⅠandcontrolgroupⅡwas98.0%,90.0%and92.0%respectively.

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简介：Objective:Toinvestigatenephroprotectiveeffectsofamixtureof8L-aminoacidsonrenalischemia-reperfusioninjuryanditseffectsonrenalendothelin-1(ET-1).Methods:Themixtureof8L-aminoacidsincludesglycine,alanine,threonine,serine,valine,leucine,isoleucineandproline.Acuteischemicrenalinjurywasinducedbyclampingrenalpediclefor45minutesinrats.SixtymaleSprague-Dawieyratswererandomlydividedinto3groups:asham-operatedgroup(GroupA,n=8),acontrolgroup(GroupB,n=26)andanaminoacid-treatedgroup(GroupC,n=26).Aminoacidswereinfusedatarateof1mi·100g-1·h-1Ihourbeforeischemiaandduring3hoursofthewholereperfusion.Theserumcreatininevalues,BUNlevels,creatiulneclearance,urinesodium&potassiumexcretion,urinelactatedehydrogenase(LDH),therateofurineflowandhistologicalexaminationweremeasured.RenalET-1levelswereassayedwithradioimmunologicalassay(RIA)Results:Thecreatinineclearancewas471.0μl/min±121.5pi/maininGroupCand227.0μl/min±27.0μl/mininGroupB3hoursafterreperfnsion,P＜0.01).Theurineflowratewas63.6pi/min±15.2μl/mininGroupCand24.3μl/min±7.7μl/mininGroupB,P＜0.01)1.5hoursafterreperfusion.Theserumcreatininewas85.0μl/min±7.7μmol/LandBUNoncentration11.4mmol/L±3.9mmol/LinGroupCand112.7μmol/L±19.5μmol/Land20.7mmol/L±6.6mmol/LrespectivelyinGroupBafter24hoursofreperfusion(P＜0.05).ThemeanhistologicalscorebystandardsofPallerinkidneyswas108.7±15.7inGroupC,and168.8±14.8inGroupB(P＜0.01).TherenalET-1levels15minuteand3hoursafterreperfusionwere7.2pg/mg±0.8pg/mgand9.6pg/ml±1.0pg/mlinGroupC,and10.1pg/ml±2.8pg/mland13.0pg/ml±2.7pg/miinGroupB(P＜0.01).Conclusions:Themixtureof8L-aminoacidscanprovideremarkableprotectionagainstrenalisehemia-reperfusioninjuryinrats.Thismayassociat

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简介：Aprimaryhumanrenalcellcarcinomawasdevelopedasaxenograft(NT-25)andmaintainedbyserialtransplantationinnudemice.TheeffectofUFTonthisneogrowthwastestedandevaluatedaswellitsdistributionintheanimaltissues.TheconcentrationofUFTwashigherintumortissuesthanthatinothertissuesandintheanimalexperimentationUFTwasfoundtobeeffectiveonhumanrenalcellcarcinoma.

简介：Objective:Tostudytheexpressionofdendriticcellsinhumanrenalcellcarcinomaandexplorethecause,sotorevealthemechanismofescapingimmunesurveillanceinRCC.Methods:TheexpressionsofCD83+DCS,CD1a+DCS,VEGFandTGF-β1intumoral,peritumoralandnormalkidneytissuesofRCCin30casesweredetectedbyimmunohistochemistryusingstreptavidin/peroxidese(SP)Results:CD83+DCSweremainlylocatedintheperitumoralareas;whereasCD1a+DCS、weremainlyretainedwithinthecancernests.ThenumberofCD83+DCSwasinverselycorrelatedwiththeclinicalstage(P<0.05);buttherewerenosignificantcorrelationsbetweenthenumberofCD1a+DCS、andtheclinicalstage(P>0.05).TheexpressionsofCD83+DCSandCD1a+DCShavesignificantdifferencebetweenthetumoral,peritumoralandnormalkidneytissues(P<0.001).TheexpressionofVEGFandTGF-β1weresignificantlylowerinsampleswithhighlyinfiltratingCD83+DCS(P<0.05);WhereasCD1a+DCSwerenot(P>0.05).Conclusion:DChasthetendencytogatheringintumor,butbecauseoftheimmunosuppressivecytokins,forexampleVEGFandTGF-β1,inhibitsthematurationofDC,therearelessmatureTIDCS(CD83+TIDCS)inthetumoraltissues,theyaremainlylocatedintheperitumoralareas.ThismaycontributetothemechanismofescapingimmunesurveillanceinRCC.

简介：HumanprimaryepithelialcellsofrenalpelviswasestablishedtoinvestigatetheadherenceofuropathogenicEscherichiacoli(UPEC)tothiscellline,inwhichtheprimarycellculturewasperformedbyusingcultivationofthenormalepitheliumofrenalpelvisinkeratinocyteserumfreemedium(K-SFM)withepidermalgrowthfactor(EGF)andbovinepituitaryextract(BPE).BothUPEC132obtainedfromurinespecimenofpatientswithpyelonephritisandthepilus-freerepresentativestrainE.coliK-12p678-54wereusedtostudytheadherenceofthesestrainsonhumanprimaryepithelialcellsofrenalpelvis.TheUPECadherencewasperformedwithobservationonthemorphologicalchangesoftheadheredcells,whiletheadhesionratesandindiceswerecalculatedindifferenttimesofexperiment.Inaddition,thevirulencegeneshlyandcnf1ofUPEC132weredetectedbymultiplexPCRassay.Inthisstudy,thehumanprimaryepithelialcellsofrenalpelviswasfoundtoexhibitthecharacterofthetransitionalepithelialcells.Comparedwiththecontrolgroup,theadhesionratesandindicesbegantoincreasefrom15minoftheexperimenttimeandreacheditspeakin120min.TheadhesionrateandindexofUPEC132tohumanprimaryepithelialcellsofrenalpelviswere74.4%and34.0respectively.ManymicroscopicchangesintheprimarycellsadheredwithUPEC132couldbedetected,suchasroundingorirregularityinshape,unevennessinstainingandthecytoplasmicandnuclearchanges.ItsuggeststhathumanprimaryepithelialcellsofrenalpelviscanbeusedfortheexperimentonUPECadhesion,thusprovidingabasisforthefurtherstudyonthepathogenesisofUPEC.

简介：ObjectivesTostudythechangesofnitricoxide,angiotensinⅡandsuperoxideanioninrenalarteryhypertensionpathogenesis.MethodsMaleWistarratsweighing256-285gweredividedinto5groupsrandomly,10ratsofeachgroup.Controlgroup:falseoperationwasmadeandroutinedietwasgiven;Ligaturegroup:leftrenalarterywasligatureduncompletelyandroutinedietwasgiven;Ligature+Losartangroup:leftrenalarterywasligatureduncompletelyandLosartan20mg·kg^-1·d^-1wasaddedinthedrinkingwater;Ligature+L-Arggroup:leftrenalarterywasligatureduncompletelyandL-Arg2g·kg^-1·d^-1wasaddedinthedrinkingwater;Ligature+L-Arg+Losartangroup:leftrenalarterywasligatureduncompletelyandL-Arg2g·kg^-1·d^-1andLosartan20mg·kg^-1·d^-1wasaddedinthedrinkingwater.Bloodpressureandheartrateweremeasuredbeforeandattheendoftheexperiment.Oneweekafterligature,bloodwasdrawntodetermineangiotensinⅡ,cGMP,nitricoxide,nitricoxidesynthase(NOS),O2^-,superoxidedismutase(SOD).ResultsSystolicbloodpressurewashigherinligaturegroupthanthatincontrolgroup(p<0.05),systolicbloodpressurewasmuchlowerinligature+Losartangroupthanthatinligaturegroup.Heartratedidnotchangesignificantlyafterexperiment(p>0.05).AngⅡwashigherinligaturegroupthanthatincontrolgroup,evenmuchhigherinligature+Losartangroup(p<0.01).TherewasnodifferenceofcGMPineachgroup(p>0.05).TheconcentrationofNOwaslowerinligaturegroup(p<0.05),NOwashigherinligature+L-Arg+Losartangroupthanthatinligaturegroup(p<0.05).O2^-washigherinligaturegroupandligature+L-Arggroupthanthatincontrolgroup(p<0.05),O2^-waslowerinligature+Losartangroupthanthatinligaturegroup(p<0.05).ThelevelofSODwaslowerinligaturegroupthanthatincontrolgroup(p<0.05),higherinligature+L-Arggroupandligature+L-Arg+Losartangroupthanthatinligature

简介：TheZ10andZ37strainsofhemorrhagicfeverwithrenalsyndrome(HFRS)virusandtheMongoliangerbil(Merionsunguiculatus)kidneycellswereusedtopreparetheinactivatedbivalentvaccine.AphaseⅡclinicaltrialuseofthisvaccinewasmadein750Chinesevolunteers.Theresultsshowedthatthesidereactionratewas2.5%andthesero-conversionrateofneutralizingantibodiesagainstHantaanandSeoulvirusesintheinoculatedvolunteerswere87.6%and96.3%respectively.

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简介：Inordertoelucidatethemolecularandimmunologicalmechanismsaswellasthepathogenesisofhemorrhagicfeverwithrenalsyndrome(HFRS),theCD8^+cytotoxicTlymphecytes(CTL)clonewasestablisheddirectlyfromperipheralbloodmononuclearcells(PBMC)ofpatientswithHFRS.TheactivitiesofCTLweredetectedasusualwithEBV-transformedlymphoblastoidcellline(BLCL)astargetcells.TheresultsshowedthattheCTLclonecouldrecognizedandkilledthetargetcellswithspecificityofnucleocapsidproteinofHantaanvirus(HTNVNP)withthecytotoxicitypercentagesof50.2%,25.4%and39.0%respectively.TheseresultsdemonstratedthattheantigenicepitopesofHTNVNPmainlylocatedontheC-temainaloftheviralnucleocapsidprotein.

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