简介：Thisstudywasconductedtodevelopandcharacterizeanovelcytoplasmicmalesterile(CMS)sourcewhichwasidentifiedfromDongxiangwildrice(Oryzarufipogon)bycrossingDongxiangwildriceasfemalewithZhongzao35,anindicainbredvariety,asmaleandcontinuousbackcrossingwithZhongzao35.ObservationunderopticalmicroscopemanifestedthatthisnovelCMSbelongedtotypicalabortiontypewithlesspollencomparedwithwildabortivetypecytoplasm(CMS-WA).SequentialplantingshowedthatthisnovelCMShascompleteandstablemalesterility.Testcrossexperimentshowedthatallthe24testedmaterialsincludingmaintainerandrestorerlinesofCMS-WAandHongliantypecytoplasm(CMS-HL)andotherindicainbredvarietiesarethemaintainerswithcompletemaintainingability,suggestingthatthisnovelCMShasfertilityrestorationtotallydifferentfromCMS-WAandCMS-HLandbelongstoanoveltypeofCMS.Sofar,weonlydiscoveredauniquefertilityrestorationsourceforthisnovelCMS.InheritanceanalysisshowedthatthefertilityrestorationofthisCMSwasgovernedbythreepairsofindependentdominantgenes.ProspectforapplicationofthisnovelCMSsysteminhybridricebreedingwasalsodiscussed.

简介：Objective:Tostudytheeffectofelectroacupuncture(EA)andVerapamilandNifedipine(calciumchannelinhibitors)onfreecalciumconcentrationsofcellsandintrasynaptosomesinhypothalamus(HT),periaqueductualgreymatter(PAG)andhippocampus(HIP)ofmice.Methods:ThefemaleICRmicewererandomlydividedintocontrol,EA,CaCl2andCaCl2+EAgroups(n=8ineachgroup).Painthresholdwasdetectedbyusingradiation-heatirradiation-inducedtailflickmethod.EA(8Hz,asuitablestimulatingstrength,dense-sparsewavesanddurationof30min)wasappliedto'Shuigou'(水沟GV26)and'Chengjiang'(承浆CV24).CaCl2(10μL,0.2μmol/L)wasinjectedintothelateralcerebralventricleofmiceafterEA.Theconcentrationsofcytosolicfreecalcium([Ca2+]i)inHIP,PAG,HTcellsuspensionspecimenandhippocampalintrasynaptosomesuspensionofmiceweredeterminedbythefluorescentcalciumindicatorFura-2-AMandaspectrofluorometer.Results:DuringEAanalgesia,theintracellularfree[Ca2+]iinHTandPAGspecimensandintrsynaptosomal[Ca2+]iofthe3cerebralregionsdecreasedconsiderably(P<0.05～0.01),butthatinhippocampalcellsuspensionincreasedsignificantly(P<0.01)incomparisonwithcontrolgroup.Theconcentrationsofhippocampalintrasynaptosomalfree[Ca2+]idecreasedsignificantlyafteraddingVerapamilandNifedipinetotheextractedhippocampalintrasynaptosomalspecimen.MicroinjectionofCaCl2intolateralventriclehadnoapparentinfluenceondegreeofanalgesia(DA)%andintracellularandintrasynapsotomal[Ca2+]i,butsignificantlylowerDA%andreducechangesofcytosolicandintrasynaptosomal[Ca2+]iinducedbyEAstimulation.Conclusion:CalciumionintheneuronsandintrasynaptosomeofHT,PAGandHIPisinvolvedinelectroacupunctureanalgesia.

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简介：TheDNAfragmentsabout1600bpwereamplifiedusingrandomamplifiedpolymorphismDNA(RAPD)primerOPA12withthetemplatesofmitochondrialDNAofZhenshan97AandZhenshan97B,andweresequenced.ThenucleotidesequencesandlengthsofthefragmentsfromZhenshan97AandZhenshan97Bshowednodifference.Thepreciselengthofthefragmentwas1588bp.Sequencecharacterizedamplificationregion(SCAR)primerswerethendevelopedtodiscriminatethecytoplasmicmalesterile(CMS)linesandtheirmaintainerlines.Aspecific1588bpfragmentcouldbeamplifiedwithSCARprimers,CHI19F2/CHI19R2andCHI20F3/CHI23R3,inthemitochondrialDNAofZhenshan97A,butnotZhenshan97B.Furthermore,thespecificfragmentcouldbealsoamplifiedfromthetotalDNAfromgreenleaftissuesofZhenshan97AwithSCARprimers,butnotZhenshan97B.Withthecorrespondingprimers,thespecificfragmentcouldalsobeamplifiedfromthetotalDNAofgreenleavesofothertwoCMSlineswithwildabortivetypecytoplasm(CMS-WA),namelyZhenpinAandTianfengA,butnotintheirmaintainerlines.Moreover,usingtotalDNAastemplate,eachofthefourpairsofSCARprimerscouldalsobeusedtoamplifythe1588bpfragmentinCMS-ID(Indonesiapaddytype)lineⅡ-32A,butnotinII-32B,andthespecificfragmentwasamplifiedfromtheDNAofbothF1andF2seedlingsofShanyou63.Theresultsofdetectingthegeneticpurityofaman-mademixtureoftheseedsofZhenshan97AusingCHI20F3/CHI23R3werecompletelyconsistentwiththephenotypes.Takentogether,theseresultsindicatedthatthespecific1588bp-fragmentamplifiedbyCHI20F3/CHI23R3wastheuniqueamplificationproductsofCMSmitochondrialDNA,andcouldbeusedtodistinguishCMS-WAandCMS-IDlinesfromtheircorrespondingmaintainerlinesattheseedlingstage.

简介：为了在不同发育阶段调查织物transglutaminase活动，叶子和米饭的年轻圆锥花序的变化，从Honglian-typecytoplasmic不育系，YuetaiA和它的维护者线被切除，YuetaiB分别地。为在米饭的织物transglutaminase活动察觉的一个ELISAmeasurement协议很好被建立。结果显示织物transglutaminase活动被钙阳离子，和织物transglutaminase活动在衰老的叶子断然调整在年轻叶子比那显著地高。没有不同差别在YuetaiA和YuetaiB之间被注意。而且，从四个起核心作用到双性人织物transglutaminase活动逐渐地增加了的阶段年轻圆锥花序发展并且直到在双性人的最大值的withtheprogression起核心作用在YuetaiA.However的舞台，没有类似的变化在YuetaiB被观察。这显示tissuetransglutaminase涉及房间在败育花粉的规划死亡。

简介：AbstractBackground:Follistatin-like 1 (FSTL1) plays both pro-inflammatory and anti-inflammatory roles in the inflammatory processes. We investigated whether serum FSTL1 could predict the current anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV)-specific indices.Methods:We randomly selected 74 patients with AAV from a prospective and observational cohort of Korean patients with AAV. Clinical and laboratory data and AAV-specific indices were recorded. FSTL1 concentration was determined using the stored sera. The lowest tertile of the short-form 36-item health survey (SF-36) was defined as the current low SF-36. The cutoffs of serum FSTL1 for the current low SF-36 physical component summary (PCS) and SF-36 mental component summary (MCS) were extrapolated by the receiver operator characteristic curve.Results:The median age was 62.5 years (55.4% were women). Serum FSTL1 was significantly correlated with SF-36 PCS (r = -0.374), SF-36 MCS (r = -0.377), and C-reactive protein (CRP) (r = 0.307), but not with Birmingham vasculitis activity score (BVAS). In the multivariable linear regression analyses, BVAS, CRP, and serum FSTL1 were independently associated with the current SF-36 PCS (β = -0.255, β = -0.430, and β = -0.266, respectively) and the current SF-36 MCS (β = -0.234, β=-0.229, and β= -0.296, respectively). Patients with serum FSTL1 ≥779.8 pg/mL and those with serum FSTL1 ≥841.6 pg/mL exhibited a significantly higher risk of having the current low SF-36 PCS and SF-36 MCS than those without (relative risk 7.583 and 6.200, respectively).Conclusion:Serum FSTL1 could predict the current functional status in AAV patients.

简介：YuetaiA的种子样品，一种Honglian(HL)类型细胞质的男性无菌(厘米)在混合大米的线被调查估计种子纯净并且由在南京，江苏省和Lingshui模仿生物沾染物到YuetaiA分析离开类型植物的原因，海南省在20042006期间。YuetaiA的种子杂质主要源于在生物污染以后然而并非从它的绝育unstability恢复富饶基因(Rf)的基因飘移。十根维护者线，五根restorer线和三thermo敏感的遗传因子的男性，无菌的线在学习使用了的所有能传花粉给生产F1的YuetaiA和YuetaiB种，直接或间接地导致漂流进YuetaiA并且产生iso细胞质恢复产生的Rf基因。而且，高outcrossing率和有许多变化的YuetaiA的类似的标题日期容易在米饭生产力量使用了在生物污染的结果。在移开所有植物，Rf基因在YuetaiA混合了并且阻止Rf基因漂流进YuetaiA以后，YuetaiA和YuetaiB的种子纯净被提起了到100%。