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简介：AbstractBackground:Anterior thalamic nuclei (ATN) deep brain stimulation (DBS) is an effective method of controlling epilepsy, especially temporal lobe epilepsy. Mossy fiber sprouting (MFS) plays an indispensable role in the pathogenesis and progression of epilepsy, but the effect of ATN-DBS on MFS in the chronic stage of epilepsy and the potential underlying mechanisms are unknown. This study aimed to investigate the effect of ATN-DBS on MFS, as well as potential signaling pathways by a kainic acid (KA)-induced epileptic model.Methods:Twenty-four rhesus monkeys were randomly assigned to control, epilepsy (EP), EP-sham-DBS, and EP-DBS groups. KA was injected to establish the chronic epileptic model. The left ATN was implanted with a DBS lead and stimulated for 8 weeks. Enzyme-linked immunosorbent assay, Western blotting, and immunofluorescence staining were used to evaluate MFS and levels of potential molecular mediators in the hippocampus. One-way analysis of variance, followed by the Tukey post hoc correction, was used to analyze the statistical significance of differences among multiple groups.Results:ATN-DBS is found to significantly reduce seizure frequency in the chronic stage of epilepsy. The number of ectopic granule cells was reduced in monkeys that received ATN stimulation (P < 0.0001). Levels of 3′,5′-cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) in the hippocampus, together with Akt phosphorylation, were noticeably reduced in monkeys that received ATN stimulation (P = 0.0030 and P = 0.0001, respectively). ATN-DBS also significantly reduced MFS scores in the hippocampal dentate gyrus and CA3 sub-regions (all P < 0.0001).Conclusion:ATN-DBS is shown to down-regulate the cAMP/PKA signaling pathway and Akt phosphorylation and to reduce the number of ectopic granule cells, which may be associated with the reduced MFS in chronic epilepsy. The study provides further insights into the mechanism by which ATN-DBS reduces epileptic seizures.

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简介：AbstractPurpose:Severe damage to the femoral head in patients with osteonecrosis has a high impact on morbidity. Despite early diagnosis, the treatment outcome is still unsatisfactory. This study aimed to explore the expression of vascular endothelial growth factor (VEGF) and cyclic guanine monophosphate (cGMP) serum level as the risk factors of femoral head osteonecrosis in alcohol-exposed Wistar rats.Methods:This was an experimental study using randomized post-test only control group design, with samples using 10-14 weeks Wistar male rats. Rats were then divided into 6 groups: 3 groups without intervention, and 3 groups with intervention using 40% alcohol given perorally. Each one group from intervention and control group was euthanized by the end of the week for 3 consecutive weeks. Proximal femurs were examined under microscope for osteonecrosis, immunohistochemically for VEGF, and blood serum for cGMP levels.Results:VEGF expression in the femoral head of alcohol-exposed Wistar rats was lower than those not exposed to alcohol (p < 0.005). Blood serum cGMP levels of alcohol-exposed Wistar rats were higher than those not exposed to alcohol (p < 0.005). The number of necrotic osteocytes in the femoral head of Wistar rats exposed to alcohol was greater than those not exposed to alcohol (p < 0.005). There are significant differences between VEGF, cGMP levels, and number of necrotic osteocytes in the control group and treatment at 1st, 2nd, and 3rd week (p < 0.005).Conclusions:Based on the result of this study, VEGF and cGMP may be considered as diagnostic biomarkers for alcohol-induced femoral head osteonecrosis.

简介：Cyclicsixmembereda-aminonitrilehaveprovedtobeveryversatilesyntheticintermediatesandhavebeenwidelyusedintheconstructionofalargenumberofindolealkaloids.Inordertoobtainsomeinformationaboutthemechanisnofelectrochemicalsynthesisofaaminonitrile.ElectrochemistrybehaviorsthatincludecyclicvoltammetryandlinearsweepvoltammetryofcyclictertiaryamineswhichincludingN-benzylpiperidine(NBP),1-(l-Methoxycarbonylethyl)piperidine(MCEP),N-methylcarbonylppiperidine(NMCP),Nethylpiperidine(NEP)wasstudied.

简介：Sofar,theacyclichypergraphhastwodifferentdefinitions.Oneisbasedonthecyclomaticnumberofthehypergraph,whereastheotherarisesfromtheacyclicschemaoftherelationaldatabaseinthecomputerscience.Inthispaper,itisfirstprovedthatthesetwodefinitionscoincidewitheachothercompletely.ThenweprovethatahypergraphHisnotacyclic,orcyclic,ifandonlyifitcontainsaspecialpartialhypergraphnamedhypercircuit.Inaddition,weshowthatHhasl(H)differenthypercircuits,wherel(H)isaparameterusedtodecidewhetherHisacyclicorcyclic.

简介：Abstract.LetGbeasimplegraph.ThecyclicbandwidthsumproblemistodeterminealabelingofgraphGinacyclesuchthatthetotallengthofedgesisassmallaspossible.Inthispaper,someupperandlowerboundsoncyclicbandwidthsumofgraphsarestudied.

简介：AsimplecyclicelastoplasticconstitutivemodelforsandisproposedbasedontheUHmodelforoverconsolidatedclay.Theproposedmodelhasthefollowingfeatures.First,inordertodescribethestress-inducedanisotropyinsand,arotationalhardeningruleisintroducedfortheevolutionoftheyieldsurfaceaxiswithdevelopmentofplasticdeviatoricstrainintheprincipalstressspace.Second,therelationshipbetweentherotationalaxisandstress-inducedanisotropyismodeledbyintroducingtheslopeofrotationalaxisintotheyieldfunction.Theflatnessoftheyieldsurfacecanbedeterminedbytheslopeofrotationalaxis.Finally,arevisedunifiedhardeningparameterisproposedtoincorporatethestress-inducedanisotropy.Themodelcapabilityindescribingthecyclicresponseofsandisverifiedbycomparingthesimulationswithavailabletestresults.

简介：SulfatecanbeactivatedbyATPsulfurylaseandadenosine5-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSKIgenewasclonedanditsactivitywasanalyzed.OsAPSKIC36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSKanditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3'-phosphoadenosine-5'-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.

简介：SulfatecanbeactivatedbyATPsulfurylaseandadenosine5’-phosphosulfatekinase(APSK)invivo.RecentstudiessuggestedthatAPSKinArabidopsisthalianaregulatedthepartitionbetweenAPSreductionandphosphorylationanditsactivitycanbemodulatedbycellularredoxstatus.InordertostudyregulationofAPSKinrice(OsAPSK),OsAPSK1genewasclonedanditsactivitywasanalyzed.OsAPSK1C36andC69werefoundtobetheconservedcounterpartsofC86andC119,whichinvolvedindisulfideformationinAtAPSK.C36A/C69AOsAPSK1doublemutationwasmadebysitedirectedmutagenesis.OsAPSK1anditsmutantwereprokaryoticallyover-expressedandpurified,andthenassayedforAPSphosphorylationactivity.OsAPSK1activitywasdepressedbyoxidizedglutathione,whiletheactivityofitsmutantwasnot.Furtherstudiesinthecasethatoxidativestresswillfluctuateinvivo3’-phosphoadenosine-5’-phosphosulfatecontent,andallAPSKisoenzymeshavesimilarregulationpatternsarenecessarytobeperformed.

简介：Combiningtheinhibitedaptazymeandmolecularbeacon(MB),wedevelopedaversatilesensingstrategyforamplifieddetectionofadenosine.Inthisstrategy,theadenosineaptamerlinkstothe8-17DNAzymetoformanaptazyme.Ashortsequence,denotedasinhibitor,isdesignedtoformaduplexspanningtheaptamer–DNAzymejunction,whichblocksthecatalyticfunctionoftheDNAzyme.Onlyinthepresenceoftargetadenosine,theaptamerbindstoadenosine,thustheinhibitordissociatesfromtheaptamerportionoftheaptazymeandcannolongerformthestableduplexrequiredtoinhibitthecatalyticactivityoftheaptazyme.ThereleasedDNAzymedomainwillhybridizetotheMBandcatalyzethecleavageinthepresenceofZn2+,makingthefluorophoreseparatefromthequencherandresultinginfluorescencesignal.Theresultsshowedthatthedetectionmethodhasadynamicrangefrom10nmol/Lto1nmol/L,withadetectionlimitof10nmol/L.

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简介：LetRbeafinitechainringwithmaximalidealγandresiduefieldF,andletγbeofnilpotencyindext.ToeverycodeCoflengthnoverR,atowerofcodesC=(C:γ0)(C:γ)(C:γi)(C:γt-1)canbeassociatedwithC,whereforanyr∈R,(C:r)={e∈Rn|re∈C}.UsinggeneratorelementsoftheprojectionofsuchatowerofcodestotheresiduefieldF,wecharacterizecycliccodesoverR.ThischaracterizationturnstheconditionforcodesoverRtobecyclicintooneforcodesovertheresiduefieldF.Furthermore,weobtainacharacterizationofcycliccodesovertheformalpowerseriesringofafinitechainring.

简介：这份报纸处理当模特儿，分析和优化真实工业问题的一种特定的类型。问题的这个班作为周期在文学被知道升起安排问题(CHSP)。在问题的如此的班上，几个工作不得不根据一个订的洗澡序列通过一根生产线流动。CHSP在生产设备出现完成大量生产并且在行动的一个重复序列找hoist。在这份报纸，我们开发P时间的Petri网模型代表行为并且验证基本生产线的某些质的性质。后来，生产线的复杂配置被建模并且他们的性质象工作需要的reachability(周期的操作)那样，没有僵局，资源分享和管理被检查并且验证。数学分析和所有建议Petri网络模型的模拟研究用Petri网络和一个视觉对象网络++工具的数学臀部被执行。纸的第二部分处理线性编程建模优化每种线配置处理的一个混合整数的发展。有周期的处理序列的学习系统的最佳的生产计划被定义，每种配置的最佳的周期的安排的可行性被证明。

简介：到进堆积的无弹性的行为的获得卓见，在干燥的沙嵌入并且使遭到了到周期的侧面的装载的垂直堆积的反应在在Laboratoire中央desPontsetChaussées进行的离心机测试试验性地被学习。周期的装载的三种类型被使用，二不对称并且关于卸掉的堆积对称的。有深度的土壤僵硬的一个近似方形根的变化从间接飞行中的密度大小，重新组成的样品上的实验室测试，和生长得很好的实验关联被获得。测试用一个周期的非线性的Winkler春天模型被模仿，它描述无弹性的现象的完整的范围，包括堆积从并且到土壤的分离和重新附件。模型由能够复制的三个数学表达式组成许多单音的补品和周期的试验性的p-y弯。参数图形地被解释的关键模型并且与土壤行为有关的物理意思。有离心机测试结果的比较表明捕获堆积土壤相互作用的几个特征的模型和它的能力的一般有效性，包括：在装载的一个早阶段玷污plastification，在堆积，和僵硬和力量的上面的部分附近由于一个松驰地区的形成“拧”行为由于周期的装载变化。从里斯等的古典曲线的曲线从测试结果和建议模型导出的p-y的比较，以及那些。(1974)为沙，也被介绍。

简介：ThecompositionandstructureofcyclicsulfoxidederivativePd(Ⅱ)complexwereinvestigated.Thecoordinatednumberwasstudiedwithslopemethod.Thecoordinationnumberis2inloweracidity,butitis3inhigheracidity.Fourmethods,UV(ultraviolet)spectra,FrIR(Fouriertransforminfrared)spectra,1H-NMR(nuclearmagneticresonance)spectra,and13C-NMRspectra,wereusedtodeterminethecoordinatedatomincomplex.PdiscoordinatedwithOandSatominS=Ogroupinloweraciditymedia.Theconversionofcoordinationbondappearswithanincreasingtime.PdiscoordinatedwithSatominS=Ogroupinhigheraciditymedia,andinter-ligand-transferreactionoccurs.

简介：Thereactionofcrosseddiperoxidationbetweenthecyclicketonesandthealkylketoneswasinvestigated.Thereactionisacompetingindifferentketones.Itisslightlyfavorabletoresultingincrossedketonediperoxideandtoresultingincrossedcycloketonediperoxidewhenthecycloketonereactswiththealiphaticketone.

简介：Microwaveradiationwasappliedtothedetectionofmetamizoiesodiumbycyclicvoltammetry.TheelectrochemicalcharacteristicsofmetamizoiesodiumwerestudiedbycyclicvoltammetryatGCelectrodeundermicrowaveradiationandaconsiderablecurrentenhancementwasobservedformetamizoiesodiuminaqueous0.05mol/LH_2SO_4.Undertheoptionalconditions,metamizoiesodiumwasdeterminedintheabsenceandpresenceofmicrowaveactivation.Intheabsenceofmicrowaveactivationcyclicvoltammogramofmetamizoiesodiumshowsgoodlinearrelationshipinaconcentrationrangeof8.0×10~(-5)—1.0×10~(-3)mol/Linaqueous0.05mol/LH_2SO_4withadetectionlimitof6.75×10~(-6)mol/L(S/N=3)andtheequationoflinearregressionisI_p=12.973c-0.1905(R~2=0.9996,n=6);inthepresenceof80Wmicrowaveactivationcyclicvoltammogramofmetamizoiesodiuminshowsgoodlinearrelationshipinaconcentrationrangeof4.0×10~(-5)—1.0×10~(-3)mol/Linaqueous0.05mol/LH_2SO_4withadetectionlimitof4.41×10~(-6)mol/L(S/N=3)andtheequationoflinearregressionisI_p=25.107c-0.1193(R~2=0.9973,n=7).Thecurrentinthepresenceof80Wmicrowaveactivationincreasestoabout2ordersofmagnitudecomparedwiththatintheabsenceofmicrowaveactivation.Theproposedmethodinthepresenceofmicrowaveactivationshowedhighselectivityandsensitivity,andthesamplingofthedisposalmethodissimple.ThemethodwasverifiedbythedeterminationofMetamizoieSodiumtabletwithsatisfactoryresults.

简介：UsingFX-4000strainunit,theprolierationinhumanlungadenocarcinomaA549cellsthatunderwentmechanicalstrainofdifferentwaveform、frequencyanddurationwerestudied.Imageanalysisrevealedthatcellularproliferationrate(PR)reducedsignificantlyaftercellsweresubjectedtosquarewavewith0～20%elongationatfrequency30,40,50and60cycles/minfor2h.ThePRhadnodistinctdifferenceatheartwave,trianglewaveandsinewavegroupcomparedwithcontrol.ItisconcludedthatsquarewaveandhigherfrequencyplayanimportantroleininhibitingA549cellsproliferation.

简介：在这份报纸，我们建立周期的减小(CR)的集中结果为一个班的算法弱没有第n个最大的特征值的部分复合都是的假设的二次的矩阵多项式等于到2的overdamped。我们的结果能被郭，Higham和Tisseur认为是那的补充[暹罗J。矩阵肛门。Appl，30(2009)，pp。1593-1613]。数字例子显示CR算法的集中行为被我们的理论大部分支配。