简介:用pCAMBIA1305.2做载体,构建含有甘菊(Dendranthemalavandulifolium)DIBADH1基因(登录号:DQ011151)启动子DBP12(登录号:DQ497621)的质粒pCHW-2。利用叶盘转化法,通过农杆菌EHA105菌株介导,将重组质粒导入烟草叶片。采用GUS组织活性检测法鉴定烟草转基因植株,结果表明:船8J2启动子驱动的GUS基因表达无组织特异性。对转基因植株进行NaCl、ABA、SA处理,GUS荧光测定发现:100μmol/LABA胁迫处理24h和400mmol/LNaCl胁迫处理48h,转pCHW-2烟草植株叶片中GUS酶活性均有大幅度提高。该结果表明:DlBADH1基因启动子DBP12是一个诱导型启动子。这一研究结果为进一步研究甘菊中BADH基因调控表达机理提供了参考。
简介:Co,Ni,CrandVin25typicalsoilsofChinawerefractionatedintoexchangeable,carbonatebound(calcareoussoils),Mnoxidebound,organicallybound,amorphousFeoxidebound,crystallineFeoxideboundandresidualformsusingaseven-stepsequentialextractionprocedure,soastostudythedistributionofchemicalformsofthesemetalsaswellastheeffectsofsoilproipertiesonthedistribution.TheresultsshowedthatmostofsoilCo,Ni,CrandVwerepresentinresidualforms,andthedistributionratioaveraged48.2%forCo,53.0%forNi,81.5%forCrand68.7%forV.Thespeciationofheavymetalswasgreatlyinfluencedbysoilphysico-chemicalpropertiesandthechemistryofelements.Theresultsalsoindicatedthattherecoveryofmetalelementsbythesequentialextractionprocedurewassatisfactory,withtherelativedifferencebetweenthesumofsevenformsandthetotalcontentinsoilsaveraging9.5%forCo,12.8%forNi,6.6%forCrand7.2%forV.
简介:AdsorptionofCr(VI)andp-chloroanilineonthreetypicalsoilcolloidsandpHinfluencewerestudiedusingbatchequilibriummethod.BothofCr(VI)andp-chloroanilineadsorptiononthecolloidscouldbewelldescribedbygeneraladsorptionsimulationequations,TheadsorptionprocesseschangedwithmediapH.WhenCr(VI)andp-chloroanilinecoexistedonsoilcolloids,theirinteractionscouldbeobservedinacertainpHrangetobeaccompaniedwithCr(VI)reduction,whichclearlysuggestedthatasurfacecatalyticreactionoccurredinthissystem.SoilcolloidactedasaneffcientcatalystfortheinteractionofCr(VI)andp-chloroaniline.ThepHvaluesatwhichnointeractionwasobservedwere4.0,4.5and5.0forthecolloidsofindigoticblacksoil,yellow-brownsoilandlatosol,respectively,Capillaryelectrophoresisusedtoanalyzep-chloroanilineprovidedahighseparationefficiencyandshortseparationtime,andneedednomoreextensivepretreatmentofsamples.
简介:为探索辣椒新品系ZJ1-12的高产优化农艺措施,运用4因素(完全实施)5水平的二次正交旋转回归设计研究了密度、氮、磷、钾肥对辣椒新品系ZJ1-12产量和经济效益的影响。结果表明:各因子表现正向增加作用,作用大小排序为密度〉K20〉N〉P2O5肥用量。单产达156.93kg/667m2以上的优化农艺措施为密度10235~10813株/667m2,纯N量13.525~14.855kg/667m2,P2O58.232~9.748kg/667m2,K2010.875~12.6kg/667m2。当最高产量为278.84kg/667m2时,纯收益高达4149.04元/667m2。