简介:[目的]应用胎儿脐动脉血流S/D值监测≥孕36周住院孕妇,观察S/D值≥3时,对新生儿窒息和发生高危妊娠的临床意义.[方法]对1510例临床资料用脐血流S/D值,与胎心电监护(NST)和B超羊水量的测定进行分析比较.[结果]脐血流S/D值≥3组对新生儿窒息的阳性符合率均高于NST无反应组和B超异常组(P<0.005).S/D值(≥3和<3)两组中发生高危妊娠率比较差异有显著性(P<0.005).[结论]脐血流S/D值检测是一种对母婴无损伤的有效监测手段,弥补了单纯用NST或B超预测判断新生儿窒息的不足,用脐血流S/D值检测配合NST和B超的综合方法来提高对胎儿监测的可靠性.
简介:“公共场所空气中可吸入颗粒物(PMl0)测定方法—光散射法”己作为卫生部标准正式发布实施。本文介绍了为执行该标准新研制的LD一3C型微电脑激光粉尘仪的性能指标和技术特点,并通过介绍激光粉尘仪质量浓度转换系数K值的测定实例,说明该仪器在公共场所空气质量检测中应用的可行性。
简介:Paget'sdiseaseofboneisafocaldisorderofboneremodellingcausedbyabnormallyincreasedosteoclast-mediatedboneresorption.qtleaffectedboneschangeinshaw,sizeanddirection,whiletherestoftheskeletonremainsnormal.TheclinicalconsequencesofthediseasewerefirstdescribedbySirJanlesPagetin1876(Figure1).
简介:Objective:Toobservetheeffectofelectroacupuncture(EA)onMeniere'sdisease.Methods:Atotalof114cases(38malesand76females)ofMeniere'sdiseaseweretreatedwithEAofErmen(耳门TE21),Tinggong(听宫SI19),Tinghui(听会GB2),Yifeng(NFDB1风TE17),Fengchi(风池GB20)andBaihui(百会GV20).Thetreatmentwasgiven3timesaweek,9timesaltogether.Follow-upwasmadeattheendofthe3rd,6thand12thmonthsaftertreatment.Results:Follow-upin114patientsshowedthatafterEAtreatment,63cases(55.26%)werecured,30(26.31%)hadremarkableimprovementintheirsymptoms,10(8.77%)respondedwithimprovementand11(9.65%)failedinthetreatment,withatotaleffectiverateof90.35%.Conclusion:EAtreatmentwaseffectiveincontrollingMeniere'sdiseasepatients'symptomsandreducingattack.
简介:Aim:Tostudypercutaneousabsorptionandmetabolismofketoprofenisopropylester(KPE)viaexcisednudemouse'sandmonkey'sskin.Methods:Excisedskinwaspreparedbysurgicalexcisionandenzymedigestion.Sideby-sidediffusioncellswereusedforinvitropermeationstudies.TheconcentrationsofKPEanditsmetaboliteinsampleswereassayedbyHPLC.Results:AllKPEpenetrationthroughwholethicknessskinandstrippedskinwasmetabolizedtoketoprofen(KP).theconcentrationofwhichinthereciiversolutionincreasedlinearlywithtime.Astothenudemouseskin.thesteady-statefluxofKPthroughwholethicknessskinwas2.5timesthatofKPEthroughthewhloethicknessskin,buttheKPandKPEremaininginthewholethicknessskinafterthefinishingofKPEpenetrationwas22.2timesincomperedwiththeKPremaininginthewholethicknessskinafterthefinshingofKPpenetration.TherateofformationofthesteadystateKPfromKPEthroughtdermiswassignificantlylowerthanthatofKPEthroughthewholethicknessskin.Inhemonkeyskin,therateofformationofthesteady-stateKPfromKPEthroughthewholethicknessskinwas0.7timesthatfromKPEthroughstrippedskin.TheKPandKPEremaininginthewholethicknessskinafterthefinishingofKPEpenetrationwas2.0timethatinthestrippedskinafterthefinishingofKPEpenetration.Therateoffornationofthesteady-stateKPfromKPEthroughdermiswaslowerthanthatfromKPEthroughthewholethicknessskinandthestrippedskin.theKPremainingindermisafterthefinsihingofKPEpenetrationwasalsosignificantlylowerthantheKPremaininginthewholethicknessskinandthestrippedskinafterthefinishingofKPEpenetration.Conclusion:KPestersareofbenefittoimporovethelocalactionofKP.andskinesterasemetabolismmainlydevelopsintheepidermis.
简介:目的了解福建汉族人群D2S44、D10S28、D4S163VNTR多态性,为亲权关系概率计算提供基因频率。方法采用美国Lifecodes公司提供的VNTR检测试剂盒,应用RFLP技术首次对福建省9个地市103名汉族无关个体进行D2S44、D10S28、D4S163VNTR多态性研究,并将所得基因频率与中国台湾、香港、新加坡等地区进行比较。结果福建汉族人群三位点VNTR呈高度多态性,其杂合率分别为88.6%、86.3%、90.6%,呈孟德尔经典遗传,分布符合Hardy-Weinberg平衡定律。结论福建、台湾、香港、新加坡华人互为比较,D2S44、D10S28位点基因频率没有显著性差别(P>0.500)。
简介:Symptomatichepato-diaphragmaticinterpositionofabowellooporChilaiditi'ssyndromeisapeculiaranatomicalconditionmostoftenfoundbychance.Itsdescribedsymptomsrangefromintermittent,mildabdominalpainanddyspepsiatoacuteintestinalobstruction.Wereportacaseofhepato-diaphragmaticmigrationofthehepaticflexureofthecolonassociatedtoanunusual,heretoforeunreported,angina-likepainexclusivelyevokedbytheleftlateraldecubitus.Tomaximizethechanceofobservinganatomicalchangesindifferentpostures,computedtomographyofthechestandabdomenwasperformedafterairinsufflationintothecolon.Whilefrankherniationintothechestwasexcluded,thescanshowedthatthehepaticflexure-withtheinterpositionofthediaphragm-cameincontactwiththerightsideoftheheartintheleftlateral,butnotinthesupine,decubitus.Thisfindingwasreproducedbyechocardiographywhichalsoshowedvirtuallyunalteredhemodynamicsafterthechangeofposture.ECG,leftandrightventricularglobalandregionalfunctionaswellascardiacinjurymarkersalsoremainedunchangedduringthemaneuver,indicatingthatthepainevokedbythelatterwasunlikelyduetomyocardialischemia.ThiscasesuggeststhatChilaiditi'ssyndromeshouldbeincludedamongthepossible,althoughrare,causesofunexplainedangina-likesymptoms.
简介:In2001and2002,publicationoftheresultsofthreelargerandomizedcontrolledtriais(RCTs)changedourunderstandingoftherisksandbenefitsofhormonereplacementtherapy(HRT),leadingtonewguidancefromnationaladvisorybodiesonitsuse.Manyclinicaliyrelevantquestionsremainunanswered,butparadoxicallythereultsofrecenttrialsmayhavemadeitmoredifficulttodesignthestudiesnecessarytoanswerthesequestions.TheUKWISDOMtrial(oestrogen-progestogenvsplaceboinhealthywomen),forexample,hasbeendiscontinued.
简介:Overthedecade,ChineseBiomedicalEngineeringhasmaderemarkleprogressandaccumulatedmuchexperience.Animportantproblemfacingusishowtotaketheroadaheadofus.Firstofall,weshouldchangeourconventionalconceptandheadforthenewroad.Aboutthreeyearsago,wemadeaspecialreviewontheconnotationandchar-acteristicsofbiomedicalengineeringandpointedoutthatBiomedicalEngineeringwas
简介:目的构建CathepsinK(CTSK)基因慢病毒表达质粒,为进一步研究CathepsinK在人软骨细胞体外老化过程中的作用奠定基础。方法采用RT-PCR技术扩增CathepsinK基因的蛋白翻译区,通过连接、转化等分子生物学技术,将该基因克隆至慢病毒pLenti6-V5载体上,经BamHI、XhoI双酶切电泳筛选、鉴定并测序。结果从软骨细胞中成功地克隆出990bp的CathepsinK基因,并经酶切分析得到6.964Kb和1.005Kb大小的两片断,测序结果显示接头两端序列正确。结论通过基因克隆方法成功构建了CathepsinK基因表达质粒,可用于进一步的病毒包装,为深入研究该基因对软骨细胞老化的影响奠定了基础。