简介：HeparinwascovalentlyimmobilizedontopolyurethanesurfaceviaaPEGspacerbyamicrowave-assistedapproachtoimprovebloodcompatibility.Firstly,amino-terminatedpoly(ethyleneglycol)(APEG)wasrapidlygraftedontoPUsurfacewithin20minbyatwo-stepmethodinvolvingmicrowave-assistedMDI-functionalizationandsubsequentmicrowave-assistedAPEGcoupling.Then,heparinwascovalentlyimmobilizedthroughanamidelinkagebythedirectcouplingofthecarboxylicacidofheparinwiththeaminogroupofAPEGonPUsurfaceusingcarbodiimidecouplingreaction.ThesurfacestructureandpropertieswerecharacterizedbyX-rayphotoelectronspectroscopy(XPS),atomicforcemicroscopy(AFM)andwatercontactanglemeasurements.Theresultsrevealedthatheparin-immobilizedPUsurfacehadslightlyincreasedroughnessandsignificantlyimprovedhydrophilicityincomparisontotheoriginalPUsurface.Theanticoagulantactivityoffilmswasevaluatedbywholebloodclottingtime(CT)andprothrombintime(PT).Complementactivationwasassessedbydetectingcomplementfragment3aconcentrationsofserumexposedtothefilms.Theresultsrevealedthatthemicrowave-assistedheparin-immobilizedPUfilmshadexcellentantithrombogenicityandsuppressedcomplementactivation,indicatingimprovedbloodcompatibility.

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