简介：Flowcytometryandimageanalysistechniquewereusedtoquantltatethenucleiofvarioussofttissuetumors.Asinglerepresentingsectionfromsofttissuesarcomawasusedforhistologicgrading.Histologlcandcytometriccomparativeanalysesshowedthatall21benigntumorswerediploid.Among62casesofsofttissuesarcoma,45(73%)wereaneuploid.Therewasasignificantdifferenceinthenuclearareabetweenbenignandmalignanttumors(P<0.01),dlploidandaneuploidtumors(P<0.05).ThetwonewtechniquesarevaluableIncellularquantitativemeasurementforsofttissuetumors.

简介：客观：借助于高分辨率图象分析(HRIA)预言食道的上皮的发育异常的结果。方法：无征状的成年人从Linxian县的Heshun公社在1983为食管的汽球细胞学被检查。93严重发育异常和食管的122温和发育异常被选择。借助于与电视照相机装备的一台Axiomat显微镜，在染色奶头的有鳞的上皮的中间的层的保存得很好的房间的100个正常原子核随机被检验。结果：93个cytologically诊断的严重发育异常盒子，24,14和7分别地在3，5和9年里进行了到癌。在另外的48个盒子中，发育异常仍然是马厩或regressed到正常。另外的盒子被用作控制。根据染色质特征，盒子的正确诊断被HRIA在75.0%完成(18/24)，85.7%(12/14)并且(6/7)85.7%盒子检验了，分别地(P<0.001)。122cytologically诊断的温和发育异常，16,13和12个盒子分别地在3，5和9年里进行了到癌。另外的81个盒子仍然是马厩或regressed到正常。正确诊断被HRIA在93.8%做(15/16)，76.9%(10/13)并且(10/12)833%盒子检验了，分别地(P<0.001)。结论：HRIA检验的染色质原子特征能预言癌症前期的损害的结果并且从non-progressor区别progressor。它能为chemo预防试用的效率的评估被用作代理人端点biomarkers。

简介：Objective:ToevaluatethefeasibilityofDNAimagecytometry(DNA-ICM)asaprimaryscreeningmethodforesophagealsquamouscellcancer(ESCC).Methods:Atotalof5,382localresidentsaged40–69yearsfromthreehigh-riskareasinChina(LinzhouinHenanprovince,FeichenginShandongprovinceandCixianinHebeiprovince)from2008to2011wererecruitedinthispopulation-basedscreeningstudy.And2,526subjectsdeclinedtoreceiveendoscopicbiopsyexaminationwithLugol'siodinestaining,while9and815subjectswereexcludedfromliquid-basedcytologyandDNA-ICMtestrespectivelyduetoslidequality.Finally,2,856,5,373and4,567subjectswereenrolledintheanalysisforendoscopicbiopsyexamination,liquid-basedcytologyandDNA-ICMtest,respectively.Sensitivity(SE),specificity(SP),negativepredictivevalues(NPV)andpositivepredictivevalues(PPV)aswellastheir95%confidenceintervals(95%CI)forDNA-ICM,liquid-basedcytologyandthecombinationofthetwomethodswerecalculated.Receiveroperatingcharacteristic(ROC)curveswereappliedtodeterminethecutoffpointofDNA-ICMforesophagealcancer.Results:DNA-ICMresultsweresignificantlycorrelativewithesophagealcancerandprecancerlesions(χ~2=18.016,P<0.001).Thecutoffpointswere5,802,5,803and8,002basedondissimilarpathologicaltypesoflowgradeintraepithelialneoplasia(LGIN),highgradeintraepithelialneoplasia(HGIN),andESCC,respectively,and5,803waschoseninthisstudyconsideringtheSEandSP.TheSE,SP,PPV,NPVofDNA-ICMtest(cutoffpoint5,803)combinedwithliquid-basedcytology[thresholdatypicalsquamouscellsofundeterminedsignificance(ASCUS)]wereseparately72.1%(95%CI:70.3%-73.9%),43.3%(95%CI:41.3%-45.3%),22.8%(95%CI:21.1%-24.5%)and87.0%(95%CI:85.7%-88.3%)forLGIN,85.7%(95%CI:84.3%-87.1%),41.3%(95%CI:39.3%-43.3%),4.6%(95%CI:3.8%-5.4%)and98.9%(95%CI:98.5%-99.3%)forHGIN,and96.0%(95%CI:95.2%-96.8%),40.8%(95%CI:38.8%-42.8%),1.7%(95%CI:1.2%-2.2%)and99.9%