简介:Inthispaperourstudiesaboutthesequentialtestingprogramforpredictingandidentificatingcarcinogens,sequentialdiscriminantmethodandcost-effectivenessanalysisaresummarized.Theanalysisofourdatabaseofcarcinogenicltyandgenotoxicityofchemicalsdemonstratestheuncertainty.ofshort-termtests(STTs)topredictcarcinogensandtheresultsofmostroutineSTTsarestatisticallydependent.WerecommendthesequentialtestingprogramcombiningSTTsandcarclnogenicityassay,theoptimalSTTbatteries,therulesofthesequentialdiscriminationandthepreferalchoicesofSTTstorspecificchemicalclass.Forillustrativepmposesthecarclnogenicitypredictionofseveralsamplechamicalsispresented.Theresultsofcost-effectivenessanalysissuggestthatthisprogramhasvastsocial-economiceffectiveness.
简介:Objective:ToconfirmwhetherBungarusmulticinctuscrudevenominducestheapoptosisofK562tumorcellsandtofindoutthecomponentsinducingapoptosisofK562cellsfromthecrudevenom.Methods:thecrudevenomseparatedandpurifiedbycationexchangechromatography,andtheeffectofvenomsonK562wasstudiedbyMTTmethodandflowcytometry.Results:ThecrudevenombegantokillK562cellsatthan8(103ng/ml(thesurvivalratewas82.5%)concentrationandtheeffectwasmoresignificantin24hwhenadministrating8(105ng/ml(thesurvivalratewas29.4%)crudevenom.ApoptoticbodieswereobservedintheK562tumorcellsbyfluorescentmicroscopyafteradministrationof5(g/mlcycloheximide(CHX)orthepeakVIsolutionatabout8(105ng/ml.Thesameresultsweredetectedbytheflowcytometry.Asub-G1peakappearedafteradministrationofCHXorthesixthpeaksolution.Conclusion:TheauthorsfoundthatthevenomcankillK562tumorcellsintime-anddose-dependentmanner.However,thekillingeffectofthevenomisnotapoptosis.What'smore,thepeakVIsolution,acomponentofthecrudevenomcaninducetheapoptosisofK562tumorcells.
简介:徐卓立,郭军华,宋三泰,卢淑娟,吴德政DETOXIFYINGEFFECTOFLISHENG-SEONCDDPANDITSRELATIONTOMETALLOTHIONEININDUCTION¥XuZhuoli;GuoJunhua;SongSantai;...
简介:ANTICOMPLEMENTARYACTIVITYINHUMANSERUMOFLUNGCANCERPATIENTSANDITSPOSSIBLECLINICALSIGNIFICANCELiuHuirong刘慧荣LiangFeng梁峰ZhangWeif...
简介:Inthepresentstudy,thechemosensitivityofMGc80-3humangastricadenocarcinomacellswasdeterminedbymeansofcolony-formingassayandtheinvitroactivitiesof10anticancerdrugswereexaminedonthebasisoftheclinicallyachievablepeakplasmadrugconcentration.TheresultsshowedthatMGc80-3cellsweremostsensitivetomitomyc’nC,adriamycinand5-fluorouracil,beingconsistentwiththeresponsenotedinclinicalgastriccancer.Thiscelllinemayretainitsoriginaldrugsensitivityandmaybeusefulinscreeningfornewcompoundswithactivityagainstthisdisease.
简介:Objective:Tounderstandwhetherverapamil(VER)resistancedevelopmentinthemultidrug-resistantcelllineanditsmechanism.Methods:K562/ADM/VERcellsublineresistanttoverapamilwasestablishedthroughagradualincreaseofVERconcentrationinthemedia.MTTmethodwasusedtoassayresistancetoVER,crossresistancetodipyriamole(DPM),cyclosporinA(CsA)inthecells,andHPLCandspectrofluorometertodetectintracellularaccumulationofVERorADMrespectively,aswellasS-Pimmunocytochemicaltechniquefordetectionofgenesexpression.Results:Itwereobservedthat7.9-foldincreaseinVERresistance,significantlyreducedintracellularaccumulationofVERorADMandalsodevelopacrossresistancetoDPMandCsAinK562/ADM/VERcells,comparedwithitsparentcell,K562/ADM.High-levelofp-glycoprotein(pgp),middle-levelofp53,p16,waspresentintwocelllineswithoutexpressionofGSTPI,C-myc,C-myc,C-fosandC-erbB-2.Bc1-2proteinexpressionwasfoundonlyinK562/ADMcells.Conclusion:K562/ADMcellswerecapableofbeinginducedtodevelopresistancetoVER.
简介:Objective:Toinvestigatephospho-(-cateninexpressioninnon-smallcelllungcancer(NSCLC)andtostudytherelationshipbetweenphospho-(-cateninexpressionandsomeclinicalpathologicalfactors.Methods:Theexpressionofphospho-(-cateninin67primaryNSCLCcasesdetectedimmunohistochemically.Results:phospho-(-cateninwasnotexpressedinnormalbronchialmucouscellandshowedcytoplasmicandnuclearexpressioninNSCLCcell.Totalpositiveexpressionratereached62.7%,andpositiveexpressionrateofnucleuswas38.8%.Thepositiveexpressionrate(87.5%)andnuclearexpressionrateofadenocarcinoma(62.5%)wereapparentlyhigherthanthoseofsquamouscellcancer(40.0%and17.1%)(P<0.01).Expressionofphospho-(-cateninhadnorelationshiptodifferentiationdegreeandlymphaticmetastasis.Thepostoperativesurvivaltimeisnotrelatedtophospho-(-cateninexpression.(Log-ranktest,P=0.9198;P=0.6274).COXmodelanalysisshowedthattumorstageanddifferentiationareindependentriskfactorstoprognosis(P=0.001;P=0.020).Conclusion:NSCLCcellsshowpositiveexpressionofphospho-(-catenin,phospho-(-cateninnuclearexpressionisrelevanttohistologicaltypes.Thereisnodifferenceinpostoperativesurvivaltimebetweenpatientswithphospho-(-cateninpositiveexpressionandpatientswithnegativeexpression,expressionofphospho-(-cateninisnotindependentriskfactortoprognosis.
简介:雷文东,张汝刚,阎水忠,王秀琴,牟巨伟,张大为,吴Nm23GENEEXPRESSIONANDITSCORRELATIONWITHLYMPHNODEMETASTASISINHUMANLUNGCANCER¥LeiWendong;ZhangRouging;...
简介:TheinvivoeffectsofPhytolaccaacinosapoly-saccharidesI(PEP-I)onimmunologiccytotoxicityofmouseperitonealmacrophagesanditsproductionoftumornecrosisfactor(TNF)andinterleukin1(IL-1)werestudied.PEP-I80or160mgkgwasgiveniptwiceevery4day.BothdoseswerefoundtohavesignificantenhancingactivityonmacrophagescytotoxicityagainstS180sarcomacellsandmalignanttransformedfibroblastL929cells.PeritonealactivatedmacrophageswereincubatedwithLPSfor2and24hrstoinduceTNFandIL-1,respectively.TheTNFandIL-1activitiesweretestedfromcytotoxicityagainstL929cellsinanabsorbenceassayofenzymaticreactionandproliferationofthymocytesco-stimulatedassayseparately.TheoptimaltimeforTNFproductionwasfoundonday8.SignificantincreasesinTNFandIL-1wereobserved.IncomparisonoftheeffectofPEP-IonTNFwiththatofknownprimingagentBCG,therewasnodifferencebetweenthem,butPEP-IhadahigheffectonIL-1.Theseresultssug
简介:Objective:ToconstructamutantpEGFP-hTERTexpressionvector,toobserveitssteadyexpressionintransfectedhumanbladdercarcinomacelllineT24anditsroleinmolecularregulatorymechanismsoftelomerase,andtoprovideanewtargetgeneforbladdercancer.Methods:PCRamplificationwasperformedbyusingprimersbasedontheknowngenesequenceofhTERT.PCRproductionwasclonedintoplasmidpGEMT-TeasyandthesequenceofmutanthTERTgenewasanalyzed.ArecombinantmutanthTERTvector(pEGFP-hTERT)wasconstructedattheEcoRIandSalIsitesofthepEGFP-C1vector.AftertransfectingthefusiongeneintobladdercarcinomacelllineT24bycalciumphosphate-DNAcoprecipitation,thesteadyexpressionofGFP-hTERTfusionproteinwastestedbyfluorescentlightmicroscopy.TheproliferationchangesofbladdercarcinomacelllineT24weredetectedbylightmicroscopyandsenescencecorrelatedβ-galactosidasestaining.Results:IdentificationofpEGFP-hTERTbyenzymedigestionshowedthatmutanthTERTfragmenthadbeenclonedintoEcoRIandSalIsitesofthepEGFP-C1vector.ThesteadyexpressionofGFP-hTERTfusionproteinwaslocalizedinthenucleusoftransfectedcells.Expressionofsenescence-associatedβ-galactosidaseintransfectedcellsgraduallyincreasedwithextendedculturedtimeandcellgrowthwassuppressed.Conclusion:Themutant-typehTERTgenesuppressestheproliferationofbladdercarcinomacelllineT24bycompetitiveeffectontelomeraseactivity.ThissuggeststhathTERTgenemightbeasuitablegenetargetforbladdercancertherapy.
简介:Havingbeenpassedfor160generations,acelllinedesignatedasH22-F25/LwasestablishedfromamurinetumorlymphaticmetastatlcmodelH22-F25whichhadbeensetupinourcollege.Thecelllinewasinsuspensionculturewitharapidproliferationandstablegrowth.Thepeaktuneofcelldivisionandproliferationwas48and96hoursafterculture.Inaweek,thecellnumberwasIncreasedby25tunes.H22-F25/Lstillkeepsthefeaturesofapoorlydifferentiatedcancer.Itstumorinducingrate(invivo)was100%in615mice.Lymphnodemetastasisratewas50%andpulmonarymetastasisrate10%.H22-F25/LIsapopulationofheterogenetlctumorcellsIncluding2stemcelllines(themodelnumberofchromosomesbeing43in40%tumorcellsand86in32%)andsomesidelines.ThecommonmarkerchromosomesM1,M2,M3andM4werepresentinallstemandsidelines.
简介:Objective:ToinvestigatetheexpressionofE2FandBcl-2andtheclinicopathologicalsignificanceinhepatocellularcarcinoma.Methods:TheexpressionsofE2F-3andBcl-2in74patientswithhepaticcarcinoma,paracarcinomaand15patientswithlivercirrhosisweredetectedbyS-Pimmunohistochemicalstaining.Results:TheexpressionofE2Finhepaticcarcinomawassignificantlyhigherthanthatinparacarcinomaorlivercirrhosis(P<0.005),theexpressionofBcl-2inhepaticcarcinomawassignificantlyhigherthanthatinparacarcinoma(P<0.005),inwhichBcl-2expressionwaslowerthaninlivercirrhosis(P<0.05).TheexpressionofE2F-3wasrelatedwithhistologicalgrade,tumorsize,andtheexpressionofBcl-2wasrelatedwithhistologicalgrade,tumorsizeandtumornumber.TherewascorrelationbetweentheexpressionofE2F-3andBcl-2inhepaticcarcinoma.Conclusion:E2F-3andBcl-2expressionmayplayanimportantroleindevelopment,progressionandcellapoptosisoftumor.
简介:目的了解环氧化酶-2(COX-2)和基质金属蛋白酶-2(MMP-2)在甲状腺乳头状癌中的表达情况及相互关系。方法甲状腺乳头状癌和癌旁组织COX-2和MMP-2的表达采用SP免疫组化分析。结果甲状腺乳头状癌组织COX-2和MMP-2的阳性表达率为81.7%(49/60)和73.3%(44/60),20例癌旁正常滤泡上皮COX-2和MMP-2阳性表达率为5.0%(1/20)和25.0%(5/20),两者阳性表达率均以甲状腺乳头状癌中为高(P<0.01)。COX-2和MMP-2在有淋巴结转移组阳性表达明显高于无淋巴结转移组(P<0.05,P<0.01)。COX-2阳性表达强度与MMP-2的阳性表达强度之间呈显著的正相关(P<0.01)。结论COX-2和MMP-2在甲状腺乳头状癌组织中的表达密切相关,两者的高表达在甲状腺乳头状癌的发生和发展中具有重要作用。提示COX-2和MMP-2可作为临床诊断的指标和化学治疗的靶点。