简介：Objective:Tostudytheroleofconnexingene(Cx43)onthedevelopmentofgliomaandthefeasibilityofusingCx43cDNAasatargetofgenetherapyofgliomas.Methods:ParentalratC6cellsandC6cellstransfectedwithCx43cDNAwereimplantedintorightcaudatenucleusofSDratsascontrolandtransfectedgroup.RatsbearingcerebralC6gliomasweretreatedwithCx43cDNAandemptyvectorastreatedgroupandemptyvectorgroup.Thegeneralmanifestation,survivaltime,MRIdynamicscanningandhistopathologicalchangesofallratswereobserved.Insituhybridizationandimmunohisto-chemistrywereusedforexaminationofCx43mRNAanditsproteiningliomas.AveragenumberofAgNORstainingwasusedfordetectionofcellproliferationactivity,andTUNELmethodfordeterminationofcellapoptosis.Results:Allratsincontrolandemptyvectorgroupdiedofcerebralgliomaswithin3weeksafterimplantationofC6cells.Sixoutofnineratsinthetransfectedgroupandeightoutoftenratsintreatedgroupkeptalivebeyond120dayswithtotallydisappearingofthetumorfoci,exceptonetreatedrathavingalittleresidueoftumor.IngliomasoftransfectedandtreatedgroupsCx43geneexpressionwasupregulated,proliferationactivitywaslowered,However,theapoptoticcellsdidnotincrease.Conclusion:ThepresentstudyindicatesthatCx43geneisofcrucialimportanceinthedevelopmentofmalignantglioma.Itcanbeaneffectivetargetforgenetherapyofgliomas.

简介：目的：研究连接蛋白43(Cx43)基因对胶质瘤细胞增殖的抑制作用及其可能的机制。方法：以脂质体介导，将Cx43cDNA转染于内源性Cx43表达缺失的鼠C6胶质瘤细胞和人TJ905胶质母细胞瘤细胞。采用MTT法及AgNORs(核仁组成区嗜银蛋白)平均数检测细胞增殖、。TUNEL法检测细胞凋亡、划痕标记染料示踪技术检测细胞间隙连接通讯(GJIC)。应用Western蛋白印迹及免疫组化法检测CX43cDNA转染细胞前后若干与胶质瘤发生密切相关的重要生长因子及受体表达，其中包括IGF1、bFGF、PDGF、IGFBP3、EGFR。结果：胶质瘤细胞转染Cx43cDNA后细胞增殖被抑制，细胞凋亡并未增加；GJIC明显恢复。BFGF、PDGF，IGF1、IGFBP3表达显著下调，但EGFR表达无变化。结论：Cx43基因对胶质瘤细胞增殖的抑制作用与GJIC恢复以及bFGF，PDGF，IGF1，IGFBP3表达下调相关，CX43可望成为胶质瘤基因治疗的靶基因。

简介：目的探讨原发性皮肤淋巴瘤的临床特点.方法回顾性分析我院1984～1998年间收治的43例原发性皮肤淋巴瘤的临床资料.结果原发性皮肤淋巴瘤占我院同期收治淋巴瘤的0.96%.治疗方法以化和/或放疗为主,部分患者手术切除原发灶.整组病例的总有效率为85.3%,CR率73.5%,5年总生存和无病生存率分别为71.6%和62.5%.蕈样真菌病/Sezary综合征(mycosisfungoidesandSezarysyndrome,MF/SS)组的5年总生存和无病生存率分别为67.1%和52.9%,非MF/SS组为75.4%和71.4%,14例非MF/SS性T细胞淋巴瘤为67.1%和61.5%.88.8%的患者首次复发部位以皮肤为主.结论原发性皮肤淋巴瘤是少见的有独特生物学行为的结外淋巴瘤,对化放疗敏感,大多数亚型的原发性皮肤淋巴瘤预后好于相同类型的结性淋巴瘤.