简介：瞄准：检验矩阵metalloproteinase-2(MMP-2)在胃的癌症纸巾并且到的表示与淋巴节点评估它的关系微转移。方法：作者从30学习了850淋巴结resected与淋巴腺切除术经历了胃切除术用的有胃的癌的病人颠倒抄写聚合酶链反应(RT-PCR)试金除了他染色。肿瘤纸巾的MMP-2表示被免疫检测组织化学的技术(EliVision加)。结果：MMP-2表示在21是积极的(70%)在9的盒子和negative(30%)盒子。没有重要关联在象年龄，性，肿瘤地点，肿瘤直径，Lauren分类和淋巴的侵略那样的MMP-2表示和另外的变量之间被发现。相反，MMP-2表示与肿瘤渗入的深度显著地相关(P=0.022)，淋巴节点转移(P=0.030)并且肿瘤区别(P=0.043)。淋巴节点微转移在77被检测(12.5%)14的淋巴节点(46.7%)胃的癌病人。MMP-2表示在12是积极的(85.7%)有淋巴节点的14个病人微转移，并且在9(56.3%)没有淋巴节点的16个病人微转移(P=0.118)。结论：我们的结果证明那MMP-2表情与肿瘤侵略，肿瘤区别和淋巴节点转移有重要关联。MMP-2表示可以是一个重要生物特征和胃的癌的重要预示的参数。我们也断定MMP-2可以参予淋巴节点的发展胃的癌的微转移。进一步的调查被需要得出一个结论。

简介：AIM:ToinvestigatetheeffectofhepatitisBvirus(HBV)XgeneonapoptosisandexpressionsofapoptosisfactorsinXgene-transfectedHepG2cells.METHODS:TheHBVXgeneeukaryonexpressionvectorpcDNVA3-XwastransientlytransfectedintoHepG2cellsbylipid-mediatransfection.UntransfectedHepG2andHepG2transfectedwithpcDNA3wereusedascontrols.ExpressionofHBxinHepG2wasidentifiedbyPT-PCR.MTTandTUNELwereemployedtomeasureproliferationandapoptosisofcellsin.threegroups.Semi-quantifiedRT-PCRwasusedtoevaluatetheexpressionlevelsofFas/FasL,Bax/Bcl-xL,andc-mycineachgroup.RESULTS:HBVXgenewastransfectedintoHepG2cellssuccessfully.RT-PCRshowedthatHBxwasonlyexpressedinHepG2/pcDNA3-Xcells,butnotexpressedinHepG2andHepG2/pcDNA3cells.AnalyzedbyMTT,cellproliferationcapacitywasobviouslylowerinHepG2/pcDNA3-Xcells(0.08910±0.003164)thaninHepG2(0.14410±0.004927)andHepG2/pcDNA3cells(0.12150±0.007159)(P＜0.05andP＜0.01).AnalyzedbyTUNEL,cellapoptosiswasmuchmoreinHepG2/pcDNA3-Xcells(980/2000)thanHepG2(420/2000),HepG2/pcDNA3cells(520/2000)(P＜0.05andP＜0.01).Evaluatedbysemi-quantifiedRT-PCR,theexpressionlevelofFas/FasLwassignificantlyhigherinHepG2cellstransfectedwithHBxthaninHepG2andHepG2/pcDNA3cells(P＜0.05andP＜0.01).Bax/Bcl-xLexpressionlevelwasalsoelevatedinHepG2/pcDNA3-Xcells(P＜0.05andP＜0.01).Expressionofc-mycwasmarkedlyhigherinHepG2/pcDNA3-XcellsthaninHepG2andHepG2/pcDNA3cells(P＜0.05andP＜0.01).CONCLUSION:HBVXgenecanimpaircellproliferationcapacity,improvecellapoptosis,andupregulateexpressionofapoptosisfactors.TheinterventionofHBVXgeneontheexpressionofapoptosisfactorsmaybeapossiblemechanismresponsibleforthechangeincellapoptosisandproliferation.

简介：瞄准：在食道的有鳞的房间癌(ESCC)调查midkine的表示并且与clinicopathological特征分析它的关系。方法：RT-PCR和免疫细胞化学的染色被用来分别地在EC109房间检测midkinemRNA和蛋白质的表示。然后，在ESCC样品的66种情况中的midkine的表示被免疫组织化学对人的midkine用单音的同种细胞的抗体检测。结果：Midkine被RT-PCR和免疫细胞化学在EC109房间表示。免疫反应在56.1%被检测(37/66)ESCC样品。midkine的表示在肿瘤房间的细胞质被发现。尤其是，midkine的紧张在充满容器和肿瘤的入侵的边阶的区域是更强壮的。Midkine更强烈地比在中等并且糟糕区分的肿瘤在很好区分的肿瘤(76.9%)被表示(43.1%和41.2%，分别地)(P<0.05)。在midkine之间没有统计上重要的关联在ESCC的表示和性，年龄，临床的阶段，淋巴节点转移或幸存。结论：Midkine完了在ESCC表示了。它可以在肿瘤血管生成和侵略起一个作用。midkine的表示在ESCC与肿瘤细胞分化被相关。肿瘤房间区分越多糟糕，midkine表示越多weaker。

简介：

简介：瞄准：在正常检测易碎的组氨酸三个一组(FHIT)的表示，并且分析它的关系，织物CRC，和联系apoptosis的蛋白质展示clinicopathological渲染表面的织物，渲染表面的腺瘤并且渲染表面的癌症(CRC)(Bcl-2，Bax，survivin)并且在颜色的apoptosis表面的癌症。方法：FHITmRNA分析被嵌套的反向的抄写聚合酶执行链反应(RT-PCR)试金。织物微数组(TMA)被建立在80个CRC织物标本检测FHIT，Bcl-2，Bax和survivin基因的表示，16在象控制的时间的一样的时期期间渲染表面的腺瘤织物标本和16个痔(PPH)织物标本。Citrate-microwave-SP被用作免疫组织化学的方法。在clinicopathological因素之间的关系，例如区别，等级和5年的幸存率被观察。TUNEL试金被用来在80个CRC织物标本检测apoptosis索引。结果：十(38.5%)从26，CRC织物标本表示了异常FHIT抄本，任何一个都没在匹配的正常织物异常FHIT抄本被观察并且由嵌套的RT-PCR渲染表面的腺瘤织物试金。在正常的FHIT基因表示的积极的率渲染表面的织物，渲染表面的腺瘤和癌织物分别地是93.75%，68.75%和46.25%。病人的Clinicopathological分析证明减少的FHIT基因表示没与年龄，性别，浆液CEA层次，肿瘤地点和尺寸被联系，组织学的分类。然而，FHIT的表示与等级，病理学的阶段，淋巴节点转移和5年的幸存在操作以后评估的区别被相关。在CRC织物的联系apoptosis的蛋白质(Bax，Bcl-2和survivin)的积极的率分别地是72.50%，51.25%和77.50%。在CRC织物的这些联系apoptosis的蛋白质的表示与FHIT的表示被相关。在FHIT否定肿瘤的吝啬的apoptosis索引在FHIT积极肿瘤是比那显著地低的(5.41+/-0.23对0.56+/-0.10，P<0.01)。结论：FHIT基因在apoptosis的规定起一个重要作用，减少的FHIT表示在肤色的开始和前进起一�

简介：INTRODUCTIONInterventiontherapyhasbecomeoneofthemaintherapiesofhepaticcancer.Theintroductionofhepaticarterialperfusionandembolizationhasprovidedopportunitiesforasecondaryoperationonpatientswithintermediateandadvancedcancer,thusprolonging

简介：AIM:Tostudytheeffectsofglutamine(Gin)onthechangeofintestinalpermeabilityanditsrelationshiptosystemicinflammatoryresponseinearlyabdominalpostoperativepatients.METHODS:Aprospective,randomized,double-blindandcontrolledtrialwastaken.TwentypatientsundergoingabdominalsurgerywererandomizedintoGingroup(oraladministrationofglutamine,30g/d,for7d,n=10)andplacebogroup(oraladministrationofplacebo,30g/d,for7d,n=-10).Temperaturesandheartratesofallpatientsweredailyrecorded.Whitebloodcellcounts(WBC)andbiochemicalvariablesweremeasuredbeforeoperationand4and7dalterdrugadministration.Serumconcentrationsofglutamine,endotoxin,diamineoxidaseandmalondialdehydeandurinelactulose/mannito(L/M)ratioweremeasuredbeforeand7dalterdrugadministration.RESULTS:Thepatientsinthe2groupswerecomparablepriortodrugadministration.SerumGinconcentrationwassignificantlydecreasedintheplacebogroupandincreasedintheGingroup7dalterdrugadministration.UrineL/MratiowassignificantlyincreasedintheplacebogroupanddecreasedintheGingroup.Theserumconcentrationofendotoxin,diamineoxidaseandmalondialdehydewassignificantlydecreasedintheGingroupcomparedwiththoseintheplacebogroup.Temperatures,heartratesandWBCcountsweresignificantlylowerintheGingroupthanthoseintheplacebogroup.CONCLUSION:Gutisoneofthesourcesofsystemicinflammatoryresponseinabdominalpostoperativepatientsandglutaminecandecreaseintestinalpermeability,maintainintestinalbarrierandattenuatesystemicinflammatoryresponseinearlypostoperativepatients.

简介：AIM:Todeterminethebowelsymptomsassociatedwithdiabetesanddiabetes-relatedfactorsafterexcludinggastrointestinal(GI)organicdiseases.METHODS:Participantswere4738(603diabeticand4135non-diabetic)patientswhounderwentcolonoscopyandcompletedaquestionnaire.Onthedayofpre-colonoscopy,9symptoms(borborygmus,abdominaldistension,increasedflatus,constipation,diarrhea,loosestools,hardstools,fecalurgency,andincompleteevacuation)wereprospectivelyevaluatedona7-pointLikertscale.Thetest-retestreliabilityofthebowelsymptomscoresfromthebaselineandsecondquestionnaireswasanalyzedusingkappastatistics.Associationsbetweenbowelsymptomscoresanddiabetesordiabetes-relatedfactorswereanalyzedbyarank-orderedlogisticmodeladjustedforrelatedconfounders,andoddsratios(ORs)wereestimated.RESULTS:Inmultivariateanalysis,constipation[adjustedoddsratio(AOR)=1.57,CI:1.33-1.85,P<0.01]andhardstools(AOR=1.56,CI:1.33-1.84,P<0.01)wereassociatedwithdiabetes,andfecalurgency(AOR=1.16,CI:0.99-1.37,P=0.07)andincompleteevacuation(AOR=1.16,CI:1.00-1.36,P=0.06)weremarginallyassociatedwithdiabetes.ThesesymptomsremainedassociatedevenafterexcludingorganicGIdiseasesoncolonoscopy.Test-retestreliabilityofsymptomscorewithameandurationof3.2mowasgood(meankappa,0.69).Associationsofsymptomswithdiabetes-relatedfactorswerefound;constipationwithHbA1c≥8.0%(AOR=2.11,CI:1.19-3.73),bodymassindex(BMI)<25(AOR=2.11,CI:1.22-3.66),andinsulinuse(AOR=1.90,CI:1.08-3.36);hardstoolswithdiabetesduration(AOR=1.03,CI:1.00-1.07);fecalurgencywithBMI<25(AOR=1.73,CI:1.00-2.98);andincompleteevacuationwithBMI<25(AOR=2.60,CI:1.52-4.43),serumcreatininelevel(AOR=1.27,CI:1.10-1.47),andinsulinuse(AOR=1.92,CI:1.09-3.38).CONCLUSION:Diabetesisassociatedwithconstipation,hardstools,fecalurgency,andincompleteevacuation,andpoorglycemiccontrol,duration,leanness,andnephropathyaffecttheriskofthesesymptoms.

简介：AIM:Toconstructanon-resistantandattenuatedSalmonellatyphimurium(S.typhimurium)strainwhichexpressesconservativeregionofadhesionABofHelicobacterpylori(Hpylon)andevaluateitsimmunogenicity.METHODS:TheABgeneamplifiedbyPCRwasinsertedintotheexpressionvectorpYA248containingasdgeneandthroughtwotransformationsintroducedintothedeltaCya1deltaCrp1deltaAsdattenuatedSalmonellatyphimuriumstrain,constructingbalancedlethalattenuatedSalmonellatyphimuriumstrainsX4072(pYA248-AB).BridgedELISAmethodwasusedtomeasuretheexpressionofABantigeninsonicateandculturesupematant.AccordingtothemethoddescribedbyMeacock,stabilityoftherecombinantwasevaluated.Semi-lethalcapacitytestwasusedtoevaluatethesafetyofrecombinant.Theimmunogenicityofrecombinantwasevaluatedwithanimalexperiments.RESULTS:TheattenuatedS.typhimuriumX4072(pYA248-AB)whichexpressesABwassuccessfullyconstructed.Furthermore,bridgedELISAassayshowedthatthecontentofABinrecombinantX4072(pYA248-AB)culturesupematantwashigherthanthatwasinthalluslyticliquor.AndafterrecombinantX4072(pYA248-AB)wasculturedfor100generationswithoutselectionpressure,bheentirerecombinantbacteriaselectedrandomlycouldgrow,andtheABantigenwasdefectedpositivebyELISA.ThegrowthcurveoftherecombinantbacteriashowedthatthegrowthstatesofX4072(pYA248)andX4072(pYA248-AB)werebasicallyconsistent.ThesurvivalrateofC57BL/6wasstill100%,at30daftermicetakingX4072(pYA248-AB)1.0×l0^10cfuorally.OralimmunizationofmicewithX4072(pYA248-AB)inducedaspecificimmuneresponse.CONCLUSION:Invitrorecombinantplasmidappearstobestableandexperimentsonanimalsshowedthattherecombinantstrainsweresafeandimmunogenicinvitro,whichprovidinganewliveoralvaccinecandidateforprotectionandcareofHpyloriinfection.

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简介：瞄准：在老鼠碳在Kupffer房间决定激活血小板的因素(PAF)合成和它的受体表示导致四氯化物的肝硬化。方法：Kupffer房间，从控制和导致CCl4的肝脏硬化症的老鼠的肝孤立，一夜间被放在没有浆液的媒介。PAF浸透绑定，ET-1浸透和比赛绑定是assayed。导致的PAF合成，PAF的mRNA表示，preproendothelin-1，endothelinA(希腊语字母的第七字)和endothelinB(ETB)受体也是的ET-1决定了。结果：PAF合成的双重的增加(1.42+/-0.14对0.66+/-0.04pg/mugDNA)并且膜界限PAF的1.48褶层增加(1.02+/-0.06对0.69+/-0.07pg/mugDNA)在肝脏硬化症的老鼠的激活的Kupffer房间被观察。到Kupffer房间的ET-1的申请在激活的Kupffer房间经由ETB受体，而是PAF合成在肝脏硬化症、正常的老鼠以一种集中依赖者方式导致了PAF合成在正常Kupffer房间是比那更有效的。在激活的Kupffer房间，PAF受体表示和PAF绑定能力显著地被提高。激活的Kupffer房间涨了[125I]-ET-1绑定能力，而是改变的两个都不受体的亲密关系，也不希腊语字母的第七字的表达式受体。结论：在导致CCl4的肝硬化期间的Kupffer房间是增加的PAF的主要来源。ET-1内长地涉及由paracrine经由ETB受体在激活的Kupffer房间刺激PAF合成。希腊语字母的第七字受体没出现在激活的Kupffer房间，它可以加重肝硬化的肝、额外的肝的复杂并发症。

简介：例1男，17岁，因“右上腹疼痛伴发烧5天”入院。5天前无诱因右上腹疼痛，慢性钝痛持续性加剧、畏寒、发烧。查体：T39．0℃，皮肤粘膜无黄染；腹平、软，右上腹深压痛，无反跳痛，Murphy，S征(-)，肝区叩痛。B超：肝脏中部脏面探及-7×3cm大小囊性肿物，囊壁较厚，内有数块大小不等的强回声光团，正常胆囊位置未探及胆囊。输液、应用抗生素治疗两

简介：AIM:RecombinedplasmidpETNF-P16wasconstructedtoinvestigateitsexpressionpropertiesinesophagealsquamouscarcinomacelllineEC9706inducedbyX-rayirradiationandthefeasibilityofgene-radiotherapyforesophagealcarcinoma.METHODS:RecombinedplasmidpETNF-P16wasconstructedandtransfectedintoEC9706cellswithlipofectamine.ELISA,Westernblot,andimmunocytochemistrywereperformedtodeterminetheexpressionpropertiesofpETNF-P16inEC9706aftertransfectioninducedbyX-rayirradiation.RESULTS:EukaryoticexpressionvectorpETNF-P16wassuccessfullyconstructedandtransfectedintoEC9706cells.TNFαexpressionsweresignificantlyincreasedinthetransfectedcellsafterdifferentdosesofX-rayirradiationthaninthoseafter0Gyirradiation(1192.330-2026.518pg/mL,P<0.05-0.01),andtheTNFαexpressionsandP16weresignificantlyhigher6-48hafter2GyX-rayirradiation(358.963-585.571pg/mL,P<0.05-0.001).NoP16expressionwasdetectedinnormalEC9706cells.However,therewasstrongexpressioninthetransfectedandirradiationgroups.CONCLUSION:X-rayirradiationinductioncouldsignificantlyenhanceTNFαandP16expressioninEC9706cellstransfectedwithpETNF-P16plasmid.Theseresultsmayprovideimportantexperimentaldataandtherapeuticpotentialforgene-radiotherapyofesophagealcarcinoma.

简介：柿石是最常见的植物性类石,多形成于胃内,并可进入十二指肠和空肠。而食管柿石症较少见,我院在胃镜检查中发现2例,并成功地经胃镜取出,现报告如下。例1:患者,女,78岁,因吞咽困难10天于1996年3月4日入院。入院前15天患者食柿子2个后逐渐出现吞咽困难,进食后呕吐,当地地区医院拟诊“食道癌”遂来我院。体检无阳性发现。纤维胃镜插入35cm见4.0cm×3.5cm×3.5cm大小褐

简介：肝性皮质盲又称肝脑眼综合症,是终末期肝病合并肝性脑病的一种特殊表现,临床较少见。我院在近期收治2例,现报告如下：病例摘要例1女性,32岁。

简介：例1，女，58岁，发现右腹部包块3天入院。主要表现为腹胀、纳差。查体：腹部膨胀，右上腹触及一约15cm×15cm×15cm大的包块，质稍硬，活动度小，与周围边界不清，触之无压痛。腹部揉面感。实验室检查：血白细胞5．9×10^9/L，中性0．73，淋巴0．27；尿镜检正常；肝功、尿素氮均正常；B超示腹膜后肿瘤。初步诊断为腹膜后肿瘤。

简介：AIMTodeterminetheroleofcorticotropinreleasingfactorreceptor(CRF2)inepithelialpermeabilityandenterocytecelldifferentiation.METHODSForthispurpose,weusedratSpragueDawleyandvariouscoloncarcinomacelllines(SW620,HCT8R,HT-29andCaco-2celllines).ExpressionofCRF2proteinwasanalyzedbyfluorescentimmunolabelinginnormalratcolonandthenbywesternblotindissociatedcolonicepithelialcellsandinthelysatesofcoloncarcinomacelllinesorduringtheearlydifferentiationofHT-29cells(tenfirstdays).ToassesstheimpactofCRF2signalingoncoloniccelldifferentiation,HT-29andCaco-2cellswereexposedtoUrocortin3recombinantproteins(Ucn3,100nmol/L).Insomeexperiments,cellswerepre-exposedtotheastressin2b(A2b)aCRF2antagonistinordertoinhibittheactionofUcn3.Intestinalcelldifferentiationwasfirstanalyzedbyfunctionalassays:thetrans-cellularpermeabilityandthepara-cellularpermeabilityweredeterminedbyDextran-FITCintakeandmeasureofthetransepithelialelectricalresistancerespectively.Morphologicalmodificationsassociatedtoepithelialdysfunctionwereanalyzedbyconfocalmicroscopyafterfluorescentlabelingofactin(phaloidin-TRITC)andintercellularadhesionproteinssuchasE-cadherin,p120ctn,occludinandZO-1.Theestablishmentofmatureadherensjunctions(AJ)wasmonitoredbyfollowingthedistributionofAJproteinsinlipidraftfractions,afterseparationofcelllysatesonsucrosegradients.Finally,themRNAandtheproteinexpressionlevelsofcharacteristicmarkersofintestinalepithelialcell(IEC)differentiationsuchasthetranscriptionalfactorkrüppel-likefactor4(KLF4)orthedipeptidylpeptidaseIV(DPPIV)wereperformedbyRT-PCRandwesternblotrespectively.ThespecificactivitiesofDPPIVandalkalinephosphatase(AP)enzymesweredeterminedbyacolorimetricmethod.RESULTSCRF2proteinispreferentiallyexpressedinundifferentiatedepithelialcellsfromthecryptsofcolonandinhumancoloncarcinoma

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简介：Medicaltherapyfortype2diabetesmellitusisineffectiveinthelongtermduetotheprogressivenatureofthedisease,whichrequiresincreasingmedicationdosesandpolypharmacy.Conversely,bariatricsurgeryhasemergedasacost-effectivestrategyforobesediabeticindividuals;ithaslowcomplicationratesandresultsindurableweightloss,glycemiccontrolandimprovementsinthequalityoflife,obesity-relatedco-morbidityandoverallsurvival.Thefindingthatglucosehomeostasiscanbeachievedwithaweightloss-independentmechanismimmediatelyafterbariatricsurgery,especiallygastricbypass,hasledtotheparadigmofmetabolicsurgery.However,theprimaryfocusofmetabolicsurgeryisthealterationofthephysio-anatomyofthegastrointestinaltracttoachieveglycemiccontrol,metaboliccontrolandcardio-metabolicriskreduction.Todate,metabolicsurgeryisstillnotwelldefined,asitisusedmostfrequentlyforlessobesepatientswithpoorlycontrolleddiabetes.Themechanismofglycemiccontrolisstillincompletelyunderstood.Publishedresearchfindingsonmetabolicsurgeryarepromising,butmanyaspectsstillneedtobedefined.Thispaperexaminestheproposedmechanismofdiabetesremission,theefficacyofdifferenttypesofmetabolicprocedures,thedurabilityofglucosecontrol,andtherisksandcomplicationsassociatedwiththisprocedure.Weproposeatailoredapproachfortheselectionoftheidealmetabolicprocedurefordifferentgroupsofpatients,consideringtheindicationsandprognosticfactorsfordiabetesremission.

简介：目的探讨微小RNA-363(miR-363)靶向调控E2F转录因子3(E2F3)的表达对HepG2细胞增殖和凋亡的影响。方法体外培养HepG2细胞,采用Lipofectamine法将miR-363抑制剂或其阴性对照转染到HepG2细胞,继续培养48h,收获细胞,采用四噻唑蓝(MTT)法测定细胞增殖率,使用流式细胞术法检测细胞凋亡率,采用实时荧光RT-PCR法检测HepG2细胞miR-363mRNA水平,采用WesternBlot法检测HepG2细胞E2F3、BAX和Caspase-3蛋白表达水平。结果对照组HepG2细胞增殖率为(96.4±9.7)%,显著高于抑制剂处理组【(72.3±6.5)%,P<0.05】,凋亡率为(8.2±1.4)%,显著低于抑制剂处理组【(9.7±0.8)%,P<0.05】;对照组HepG2细胞miR-363mRNA相对水平为(1.0±0.1),显著高于抑制剂处理组【(0.6±0.2),P<0.05】,E2F3蛋白表达量为(1.0±0.1),显著高于抑制剂处理组【(0.6±0.1),P<0.05】,而对照组HepG2细胞Bax和Caspase-3蛋白表达量分别为(0.4±0.0)和(0.5±0.1),均显著低于抑制剂处理组【(0.6±0.1)和(0.7±0.0),P均<0.05】。结论miR-363可靶向调控E2F3的表达,抑制HepG2细胞增殖,诱导其凋亡。本研究结果为肝癌靶向治疗提供了一定的理论依据。