简介：Objective:Toestablishagoodrecoverableratmodelofcardiopulmonarybypass(CPB)tolaythefoundationforstudyingthepathophysiologyofCPB.Methods:TwentyadultmaleSprague-Dawleyratsweighing480g±20gwererandomlydividedintoCPBgroup(n=10)andShamgroup(n=10).Allratswereanaesthetized,intubatedandventilated.Thecarotidarteryandjugularveinwerecannulated.Thebloodwasdrainedfromtherightatriumviatherightjugularveinandfurthertransferredbyaminiaturizedrollerpumptoahollowfiberoxgenatorandbacktotheratviatheleftcarotidartery.Primingconsistedof8mlofhomologousbloodand6mlofcolloid.Thesurfaceofthehollowfiberoxgenatorwas0.075m~2.Ratswerecatheterizedandbroughtinbypassfor120minataflowrateof100-120ml/kg/min.Oxygenflow/perfusionflowwas0.8to1.0,themeanarterialpressure(MAP)keptin60-80mmHg.Bloodgasanalysis,lactatedehydrogenase(LDH),andsurvivalratewereexaminedsubsequently.Resnits:AllCPBratsrecoveredfromtheoperativeprocesswithoutincidentandremaineduneventfulwithinoneweek.Normalcardiacfunctionaftersuccessfulweaningwasconfirmedbyelectrocardiographyandbloodpressuremeasurements.MAPremainedstable.Theresultsofbloodgasanalysisatdifferenttimepointswerewithinanormalrange.NosignificanthaemolysiscouldbedetectedinthegiventimeframeunderbypassconditionbyusingLDH.Conclusions:TheratmodelofCPBcanprincipallysimulatetheclinicalsettingofhumanCPB.Thenon-transthoracicmodeliseasytoestablishandisassociatedwithexcellentrecovery.ThiswellreproduciblemodelmayopenthefieldforvariousstudiesonpathophysiologicalprocessofCPBandalsoofsystemicischemia-reperfusioninjuryinvivo.