简介：TocharacterizeandidentifymultipleconstituentsinDanhonginjection(DHI),afastultra-highperformanceliquidchromatographycoupledtoelectrosprayionizationquadrupoletime-of-flighttandemmassspectrometry(UHPLC-ESI-QTOF/MS)methodwasestablishedandvalidatedinthepresentstudy.Atotalof63compounds,including33phenolicacids,2C-glycosylquinochalcones,6flavonoidO-glycosides,4iridoidglycosides,6organicacids,5aminoacids,and3nucleosides,wereidentifiedortentativelycharacterized.Inconclusion,theUHPLC-ESI-QTOF/MSmethodisusefulandefficientforin-depthstructuralelucidationofchemicalcompoundsincomplexmatricesofherbalmedicinessuchasDHI.

简介：Thepresentstudywasdesignedtodevelopandvalidateasensitiveandreliableultrahighperformanceliquidchromatographycoupledwithquadrupoletime-of-flightmassspectrometry(UPLC-QTOF/MS)methodtoseparateandidentifythechemicalconstituentsofQixueShuangbuTincture(QXSBT),aclassictraditionalChinesemedicine(TCM)prescription.UndertheoptimizedUPLCandQTOF/MSconditions,56componentsinQXSBT,includingchalcones,triterpenoids,protopanaxatriol,flavonesandflavanoneswereidentifiedandtentativelycharacterizedwithinarunningtimeof42min.Thecomponentswereidentifiedbycomparingtheretentiontimes,accuratemass,andmassspectrometricfragmentationcharacteristicions,andmatchingempiricalmolecularformulawiththatofthepublishedcompounds.Inconclusion,theestablishedUPLC-QTOF/MSmethodwasreliableforarapididentificationofcomplicatedcomponentsintheTCMprescriptions.