简介：Anactivestereovisionsystembasedonamodelofneuralpathwaysofhumanbinocularmotorsystemisproposed.Withthismodel,itisguaranteedthatthetwocamerasoftheactivestereovisionsystemcankeeptheirlinesofsightfixedonthesametargetobjectduringsmoothpursuit.Thisfeatureisveryimportantforactivestereovisionsystems,sincenotonly3Drecon-structionneedsthetwocamerashaveanoverlappingfieldofvision,butalsoitcanfacilitatethe3Dreconstructionalgorithm.Toevaluatetheeffectivenessoftheproposedmethod,somesoftwaresimulationsaredonetodemonstratethesametargettrackingcharacteristicinavirtualenvironmentapttomistrackingeasily.Here,mistrackingmeanstwoeyestracktwodifferentobjectsseparately.Thentheproposedmethodisimplementedinouractivestereovisionsystemtoperformrealtrackingtaskinalaboratoryscenewhereseveralpersonswalkself-determining.Beforetheproposedmodelisimplementedinthesystem,mis-trackingoccurredfrequently.Afteritisenabled,mistrackingneveroccurred.Theresultshowsthatthevisionsystembasedonneuralpathwaysofhumanbinocularmotorsystemcanreliablyavoidmistracking.

简介：Awheeledmobilemechanismwithapassiveand/oractivelinkagemechanismforroughterrainenvironmentisdevelopedandevaluated.Thewheeledmobilemechanismwhichhashighmobilityinroughterrainneedssophisticatedsystemtoadaptvariousenvironments.Wefocusonthedevelopmentofaswitchingcontrollersystemforwheeledmobilerobotsinroughterrain.Thissystemconsistsoftwosub-systems:anenvironmentrecognitionsystemusinglinkanglesandanadaptivecontrolsystem.Intheen-vironmentrecognitionsystem,weintroduceaSelf-OrganizingMap(SOM)forclusteringlinkangles.Intheadaptivecontrol-lers,weintroduceneuralnetworkstocalculatetheinversemodelofthewheeledmobilerobot.Theenvironmentrecognitionsystemcanrecognizetheenvironmentinwhichtherobottravels,andtheadjustablecon-trollersaretunedbyexperimentalresultsforeachenvironment.Thedualsub-systemswitchingcontrollersystemisexperi-mentallyevaluated.Thesystemrecognizesitsenvironmentandadaptsbyswitchingtheadjustablecontrollers.Thissystemdemonstratessuperiorperformancetoawell-tunedsinglePIDcontroller.

简介：Thebiologicalimmunesystemisacomplexadaptivesystem.Therearelotsofbenefitsforbuildingthemodeloftheimmunesystem.Forbiologicalresearchers,theycantestsomehypothesesabouttheinfectionprocessorsimulatetheresponsesofsomedrugs.Forcomputerresearchers,theycanbuilddistributed,robustandfaulttolerantnetworksinspiredbythefunctionsoftheimmunesystem.Thispaperprovidesacomprehensivesurveyoftheliteraturesonmodellingtheimmunesystem.Fromthemethodologyperspective,thepapercomparesandanalyzestheexistingapproachesandmodels,andalsodemonstratesthefocusingresearcheffortonthefutureimmunemodelsinthenextfewyears.

简介：目的：构建4E—BPl及其T37A、T46A、$65A、T70A突变体4E—BPl-4A基因表达的重组慢病毒载体，研究其对胃癌HGC27细胞生长的影响。方法：PCR扩增4E—BPl基因及其突变体aE—BPl-4A基因并克隆到pCDH载体，构建成pCDH-4E—BPl、pCDit一4E—BPl—4A，将其-9包装载体共转染293T细胞，包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，将此慢病毒感染胃癌HGC27细胞，Western印迹鉴定病毒载体介导的4E—BPl、4E～BPl—4A蛋白的表达，MTT、克隆形成和软琼脂方法研究过量表达4E—BPl、4E—BPl—4A对胃癌HGC27细胞生长的影响。结果：包装成Lenti-4E—BPl及Lenti-4E—BPl—4A重组慢病毒载体，并将此慢病毒载体感染胃癌HGC27细胞；MTT、克隆形成、软琼脂实验表明过量表达4E—BP!可抑制胃癌HGC27细胞的生长，过量表达4E—BP!一4A时抑制效果更明显。结论：构建了4E—BPl、4E—BPI-4A的重组慢病毒表达载体，在胃癌HGC27细胞中过量表达4E—BPl可抑制细胞生长，过量表达4E—BPl-4A的抑制效果更明显。

简介：Simulatingbiologicalolfactoryneuralsystem,KⅢnetwork,whichisahigh-dimensionalchaoticneuralnetwork,isdesignedinthispaper.Differentfromconventionalartificialneuralnetwork,theKⅢnetworkworksinitschaotictrajectory.ItcansimulatenotonlytheoutputEEGwaveformobservedinelectrophysiologicalexperiments,butalsothebiologicalintelligenceforpatternclassification.Thesimulationanalysisandapplicationtotherecognitionofhandwritingnmeralsarepresentedhere.TheclassificationperformanceoftheKⅢnetworkatdifferentnoiselevelswasalsoinvestigated.

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简介：Navigationsystembasedontheanimalbehaviorhasreceivedagrowingattentioninthepastfewyears.Thenavigationsystemsusingartificialpheromonearestillfewsofar.Forthisreason,thispaperpresentsourresearchthataimtoimplementautonomousnavigationwithartificialpheromonesystem.Byintroducingartificialpheromonesystemcomposedofdatacarriersandautonomousrobots,theroboticsystemcreatesapotentialfieldtonavigatetheirgroup.Wehavedevelopedapheromonedensitymodeltorealizethefunctionofpheromoneswiththehelpofdatacarriers.Weintendtoshowtheeffectivenessoftheproposedsystembyperformingsimulationsandrealizationusingmodifiedmobilerobot.Thepheromonepotentialfieldsystemcanbeusedfornavigationofautonomousrobots.

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简介：WeintroducetherelationshipbetweenexcessnoiseinOptoelectronicCoupledDevices(OCDs)andtheirinteriordefectsandexplainhowlow-fiequencynoisecanbeusedtoestimatetheirreliability.Usingconceptsfromthebiologicalimmunesystemanditsprocessofidentifyinginvaders,wepresentasystemforestimationofthereliabilityofOCDs.Thesystemhasexpressionsfortheantigen(excessnoise),lymphocyte(criterion)andtheroleofthelymphocyteeliminatingunreliabledevices.AgeneticalgorithmwasusedtoestimatethecomponentsparametersofthenoisespectrumforestimatingthereliabilityofOCDs.Theexperimentalresultsdemonstratedthatthismethodisreliable,adaptableandpractical.

简介：Asystemisdescribedherethatcannoninvasivelycontrolthenavigationoffreelybehavingratviaultrasonic,epidermalandLEDphoticstimulatorsontheback.Thesystemreceivescommandsfromaremotehostcomputertodeliverspecifiedelectricalstimulationstothehearing,painandvisualsensesoftheratrespectively.Theresultsdemonstratethatthethreestimuliworkingroupsfortheratnavigation.Wecancontroltherattoproceedandmakerightandleftturnswithgreatefficiency.Thisexperimentverifiedthattheratwasabletoreachasettingdestinationinthewayofcablewiththehelpofapersonthroughtheappropriatecoordinationofthethreestimulators.Thetelemetryvideocameramountedontheheadoftheratalsoachieveddistantimageacquisitionandhelpedtoadjustitsnavigationpathoveradistanceof300m.Inaword,thenon-invasivemotioncontrolnavigationsystemisagood,stableandreliablebio-robot.

简介：Withtheincreaseinminingdepth,mineheatharmhasappearedtobemoreprominent.Themineheatharmcouldberesolvedorreducedbyicerefrigeration.Thus,icetransportationthroughpipelinebecomesacriticalproblem;typicallyflowresistanceoccursintheelbow.Inthepresentstudy,accordingtotheanalysisofthesurfacemorphologyoffishscale,abiomimeticfunctionalsurfacestructurefortheinteriorwallofelbowisdesigned.Basedonthetheoryofliquid-solidtwophaseflow,aCFDnumericalsimulationofice-watermixtureflowingthroughtheelbowiscarriedoutusingfiniteelementmethod.Conventionalexperimentsofpressuredropandflowresistanceforbothbionicandcommonelbowsareconductedtotesttheeffectofthebionicelbowonflowresistancereduction.Itisfoundthatwiththeincreaseintheicemassfractionintheice-watermixture,theeffectofbionicelbowonresistancereductionbecomesmoreobvious.

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简介：Thispaperproposesanewadaptivelineardomainsystemidentificationmethodforsmallunmannedaerialrotorcraft.Byusingtheflashmemoryintegratedintothemicroguidenavigationcontrolmodule,systemrecordsthedatasequencesofflighttestsasinputs(controlsignalsforservos)andoutputs(aircraft’sattitudeandvelocityinformation).Afterdatapreprocessing,thesystemconstructsthehorizontalandverticaldynamicmodelforthesmallunmannedaerialrotorcraftusingadaptivegeneticalgorithm.Theidentifiedmodelisverifiedbyaseriesofsimulationsandtests.Comparisonbetweenflightdataandtheone-steppredictiondataobtainedfromtheidentificationmodelshowsthatthedynamicmodelhasagoodestimationforrealunmannedaerialrotorcraftsystem.Basedontheproposeddynamicmodel,thesmallunmannedaerialrotorcraftcanperformhovering,turning,andstraightflighttasksinrealflighttests.

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简介：以pBRTM/HCV-3011模板,通过PCR法扩增ns5a、LISDR(左端序列)和RISDR(右端序列),分别经XhoⅠ/EcoRⅠ、XhoⅠ/HindⅢ和EcoRⅠ/HindⅢ双酶切,连入穿梭质粒pEGFP-N3中,构建重组质粒pEGFP-N3-ns5a和pEGFP-N3-ns5a-△ISDR.对重组质粒进行酶切分析和序列测定.将这2种重组质粒通过电穿孔法转染HeLa细胞,然后在G418选择压力下进行有限稀释法筛选,用RT-PCR和荧光显微镜鉴定.经酶切鉴定和基因测序证实,重组穿梭质粒已插入目的片段ns5a、LISDR和RISDR.RT-PCR和荧光显微镜检测到目的基因的表达.以上结果说明成功构建了真核表达载体pEGFP-N3-ns5a和pEGFP-N3-ns5a-△ISDR,目的基因在HeLa细胞中得到表达,为研究HCVNS5A中是否存在抗干扰素治疗的功能蛋白提供了实验材料.

简介：Thispaperproposesanautopilotsystemthatcanbeusedtocontrolthesmallscalerotorcraftduringtheflighttestforlinear-frequency-domainsystemidentification.Theinputfrequency-sweepisgeneratedautomaticallyaspartoftheautopilotcontrolcommand.Thereforethebandwidthcoverageandconsistencyofthefrequency-sweepareguaranteedtoproducehighqualitydataforsystemidentification.Besidethat,wecansetthesafetyparametersduringtheflighttest(maximumroll/pitchvalue,minimumaltitude,etc.)sothesafetyofthewholeflighttestisguaranteed.Thisautopilotsystemisvalidatedusinghardwareintheloopsimulatorforhoverflightcondition.

简介：在新一期美国《科学》杂志对“2008年值得关注的科研热点”的预测中，生命科学就占了5个席位，可以预见，2008年将又是一个生命科学研究热点年。

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简介：目的：建立一种灵敏、特异、快速的ELISA方法，用于猕猴血清中抗死亡受体5（DR5）单克隆抗体的检测。方法：采用双抗夹心ELlSA法，用猴血清吸附的羊抗入IgG包被于96孔酶标板，加入待测样品，用HRP标记的猴血清吸附的羊抗人IgG进行检测，加底物显色，读取D450值。结果：建立了检测抗DR5单克隆抗体的ELISA方法并进行了确证，方法的线性范围为12．5-800ng／mL，定量下限为12．5ng／mL，板内和板间精密度均小于15％，准确度为-4-15％，冻融稳定性和稀释稳定性良好。结论：方法学确证结果表明，本研究建立的抗DR5单克隆抗体检测方法符合新生物制品临床前药代动力学研究指导原则要求，可用于抗DR5单克隆抗体的检测。