简介：(QS)治安法官察觉到是细菌用称为autoinducers的细胞外的信号由交流的细菌的房间房间通讯进程。二个QS系统在EscherichiacoliK-12被识别了，包括一个未经触动的QS系统2那被周期的安培(营地)营地受体蛋白质(CRP)刺激建筑群和一个部分QS系统1那由对另外的微生物引起的种类产生的信号作出回应的SdiA(房间部门禁止者的suppressor)组成。在QS之间的关系在E的系统1和系统2。然而，coli仍然保持阴暗。这里，我们显示出那EAL域蛋白质，由ydiV，和营地编码了在E涉及在二个QS系统之间的相互作用。coli。sdiA和ydiV的表示被葡萄糖禁止。SdiA在一个剂量依赖者，而是nonspecific绑在ydiV倡导者区域，举止；细胞外的autoinducer1以一种sdiA依赖的方式从另外的种类刺激ydiV表示。而且，我们发现双sdiA-ydiV变化，然而并非单个变化，在导致QS系统2的抑制的细胞内部的营地集中引起2褶层减少。这些结果显示对重要环境暗示作出回应的发信号的小径例如autoinducers和葡萄糖，在E为他们的控制一起被连接。coli。

简介：Usingtwo-colourflowcytometry＞200antibodiessubmittedtothe8thInternationalWorkshopofHumanLeukocyteDifferentiationAntigens(HLDA8)havebeenanalyzedfortheirreactivitywithrestingandactivatedCD203c+basophils.Fourantibodieseithernon-reactiveorweaklyreactivewithrestingbasophilsexhibitedanincreasedreactivitywithbasophilsactivatedbyanti-IgE-mediatedcross-linkingofthehighaffinityIgEreceptor(FcεRI).TheseincludeantibodiesagainstCD164(WS-80160,cloneN6B6andWS-80162,clone67D2),aswellastworeagentswithpreviouslyunknownspecificitiesthatwereidentifiedasCD13(WS-80274,cloneA8)andCD107a(WS-80280,cloneE63-880).Theactivationpatternsfollowedeitherthe'CD203c-like'or'CD63-like'activationprofile.TheCD203cprofileischaracterizedbyarapidandsignificantupregulation(ofCD13,CD164,andCD203c),reachingmaximumlevelsafter5-15minofstimulation.ThePhosphoinositide-3-kinase(PI3K)-specificinhibitorWortmannininhibitedtheupregulationofthesemarkerswhereas12-O-tetradecanoyl-phorbol-13-acetate(TPA)inducedarapidandFcεRI-independentupregulationwithin1-2min.IntheCD63profile,maximumupregulation(ofCD63andCD107a)wasdetectedonlyafter20-40min,andupregulationbyTPAreachedmaximumlevelsafter60min.Insummary,ourdataidentifyCD13,CD107a,andCD164asnovelbasophil-activationantigens.Basedontimekineticsofupregulation,wehypothesizethatmoleculesofthe'CD203cgroup'andthe'CD63group'arelinkedtotwodifferentmechanismsofbasophilactivation.