简介:Thisreviewbeginswithabriefcommentaryonthediversityofplacentationmechanisms,andthengoesontoexaminetheextensivealterationswhichoccurintheplasmamembraneofuterineepithelialcellsduringearlypregnancyacrossspecies.Ultrastructural,biochemicalandmoregeneralmorphologicaldatarevealthatstrikinglycommonphenomenaoccurinthisplasmamembraneduringearlypregnancydespitethediversityofplacentaltypes-fromepitheliochorialtohemochorial,whichultimatelyformindifferentspecies.Toencapsulatetheconceptthatcommonmorphologicalandmolecularalterationsoccuracrossspecies,thattheyarefoundbasolaterallyaswellasapically,andthatmoreovertheyareanongoingprocessduringmuchofearlypregnancy,notjustaneventatthetimeattachment,braneduringearlypregnancyarekeytouterinereceptivity.
简介:Immatureembryosofricevarieties“Xiushui11”and“Chunjiang11”preculturedfor4dwereinfectedandtransformedbyAgrobacteriumtumefaciensstrainEHA101/pExT7(containingthespiderinsecticidalgene).Theresistantcalliweretransferredontothedifferentiationmediumandplantswereregenerated.Thetransformationfrequencyreached56%~72%measuredasnumbersofGeneticin(G418)-resistantcalliproducedand36%~60%measuredasnumbersoftransgenicplantsregenerated,respectively.PCRandSouthernblotanalysisoftransgenicplantsconfirmedthattheT-DNAhadbeenintegratedintothericegenome.InsectbioassaysusingT1transgenicplantsindicatedthatthemortalityoftheleaffolder(Cnaphalocrasismedinalis)after7dofleaffeedingreached38%~61%andthecorrectedmortalityofthestripedstemborer(Chilosuppressalis)after7dofleaffeedingreached16%~75%.Theinsectbioassayresultsdemonstratedthatthetransgenicplantsexpressingthespiderinsecticidalproteinconferredenhancedresistancetothesepests.