摘要
BACKGROUND:Duringthecellularagingprocess,thenumberofmitochondria,generationofadenosinetriphosphate(ATP),activityofrespiratorychainenzymecomplex1and4,andoxidationdecrease.OBJECTIVE:Toobservetheeffectsofaqueousandspirituousextract,aswellaspolysaccharidesfromFructusschizandrae(MagnoliaVine)onenergymetabolismandmitochondrialanti-oxidationincranialnervecellsofaD-gal-inducedagingmousemodel.DESIGN,TIMEANDSETTING:Arandomized,controlled,animalstudy.TheexperimentwasconductedattheDepartmentofBiochemistry,QiqiharMedicalCollegebetweenMarchandJuly2006.MATERIALS:Fiftyhealthy,Kunmingmiceofbothsexes,aged2-3monthsoldandweighing18-22g,wereusedforthepresentstudy.FructusschizandraewaspurchasedfromtheMedicalCollegeofJiamusiUniversity.Aqueousextracts,spirituousextracts,andpolysaccharidesfromFructusschizandraewereprepared.D-galactose(D-gal)isaproductoftheSecondReagentFactory,ShanghaiCity,China.Mn-superoxidedismutase(Mn-SOD)kit,malonaldehyde(MDA)kit,proteinquantificationkit,andinorganicphosphorustestingkitwerepurchasedfromJianChengBioeng.Co.,China.METHODS:Fiftymicewererandomlydividedintofivegroups,with10miceineachgroup:youngcontrol,agingmodel,aqueousFructusschizandraeextract,spirituousFructusschizandraeextract,andFructusschizandraepolysaccharides.Overacourseof30days,miceinagingmodel,aqueousFructusschizandraeextract,spirituousFructusschizandraeextract,andFructusschizandraepolysaccharidesgroupswereinjectedsubcutaneouslywithD-gal(100mg/kg)intothenapeoftheneckdaily,andadministeredintragastricallywithanequalvolumeofsterile,warmwater(agingmodel),aqueousFructusschizandraeextract(2g/kg),spirituousFructusschizandraeextract(2g/kg),orFructusschizandraepolysaccharides(0.2g/kg),respectively.Miceintheyoungcontrolgroupwereinjectedintothenapeoftheneckwithphysiologicalsaline
出版日期
2008年10月20日（中国期刊网平台首次上网日期，不代表论文的发表时间）
