摘要
BACKGROUND:Erythropoietinandrecombinanthumanerythropoietin(rhEPO)inhibitapoptosisofmotorneuronscausedbyspinalcordinjuryandbraindamageinrats.However,itstillremainstobeshownwhetherrhEPOcanprotectfacialmotoneurons(FMNs)aswell.OBJECTIVE:TotesttheneuroprotectiveeffectsofrhEPOoninjuredFMNs,aswellastheinfluenceonCaspase-3expression.DESIGN,TIMEANDSETTING:Randomized,controlled,animalexperiment.ThisstudywasperformedattheCentralLaboratoryofBasicMedicalCollege,ChongqingMedicalUniversityfromJanuarytoOctober2007.MATERIALS:Seventy-fivefemaleSDrats,weighing210–230g.rhEPOinjectionwasprovidedbySanshengpharmaceuticalscompany,ShenyangCity,LiaoningProvince,China,andtheLicensenumberwasHMLNS20010001.METHODS:Atotalof75femaleratswererandomlydividedintorhEPOtreatment,control,andshamoperationgroups,with25ratsineachgroup.RatmodelsoffacialnerveinjurywereestablishedintherhEPOtreatmentgroupandthecontrolgroupbycrushingthemaintrunkoftheleftfacialnerve.Surgicalmicroscopicobservationofthefacialnervedamagedisplayedperineurialdisruption.Theleftstylomastoidforamenoftheshamoperationgroupwereonlyexposed,butwithoutnerveinjury.TherhEPOtreatmentgroupwastreatedwithrhEPO(5000U/kg,i.p.)oncefollowinginjuryandonceadayfortwoweeks.Thecontrolandshamoperationgroupsweretreatedwiththesamedoseofnormalsaline(i.p.),oncefollowinginjuryandonceadayfortwoweeks.MAINOUTCOMEMEASURES:Ratsweresacrificed3,7,14,21,and28daysafterinjury,FMNsurvivalafterfacialnerveinjurywasanalyzedbyToluidinebluestaining,andthensurvivalratios(L/R)werecalculated.ThenumberofapoptoticprofilesintheinjuredFMNswereevaluatedbyTUNELstaining.ExpressionofCaspase-3inthefacialnucleuswasdetectedbyimmunohistochemistrymethods.RESULTS:Atotalof75ratswereincludedinthefinalanalysis.FMNsurvivalratios,bothin
出版日期
2008年05月15日（中国期刊网平台首次上网日期，不代表论文的发表时间）
