摘要
Methodsfordeterminingnienelowmolecular-weightoragnicacidsinrootexudatesweredevelopedbyusingreversed-phasehighperformanceliquiedchromatographywithUV(ultraviolet)detectionat214nm.Themobilephasewas18mmolL^-1kH2PO4adjustedtopH2.25withphosporicacidndtheflowratewas0.3mLmin^-1,Theanalyticalcolumnwasareversed-phasesilicabasedC-18column(shim-packCLC-ODS).Therootexudateswerecollectedthroughsubmergingthewholerootsystemintoaerateddeionizedwaterfor2hours,Thefilteredexudatesolutionswereconcentratedtodrynessbyrotaryevaporationat40℃,dissolvedin10mLmobilephase.Thechromatoraphicconditionsoforganicaciddeterminationwereanalyzed.Theresultsshowedthattherewasahighselectivityandsensitivityintheorganicaciddeterminationbyreversed-phasehighperformanceliquidchromatography.Coefficientsofvariationfororganicacieddeterminationwerelowerthan10%exceptlacticacid.Therecoverieswereconsistentlybetween80.1%to108.3%.Detectionlimitswereapproximately0.05to4.5mgL^-1fororganicacidsexceptsuccinicacidwiththedetectionlimitof7.0mgL^-1.Phosphorusdeficiencymaycontributetothereleaseoforganicacidsinsoybeanrootexudatesespeciallymalic,lacticandcitricacids.
出版日期
1998年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
