摘要
Wepreviouslyperformedtranscriptomesequencingandfoundthatgenesformatrixmetalloproteinases(MMPs),suchasMMP7and12,seemtobehighlyupregulatedfollowingperipheralnerveinjury,andmaybeinvolvedinnerverepair.Inthepresentstudy,wesystematicallydeterminedtheexpressionlevelsofMMPsandtheirregulatorsat1,4,7and14daysaftersciaticnervecrushinjury.Thenumberofdifferentiallyexpressedgeneswaselevatedat4and7daysafterinjury,butdecreasedat14daysafterinjury.Amongthedifferentiallyexpressedgenes,thosemostup-regulatedshowedfoldchangesofmorethan214,whilethosemostdown-regulatedexhibitedfoldchangesofmorethan2-10.Genesequencingshowedthat,atalltimepointsafterinjury,avarietyofMMPgenesinthe'InhibitionofMMPs'pathwaywereup-regulated,andtheirinhibitorgenesweredown-regulated.Expressionofkeyup-anddown-regulatedgeneswasverifiedbyquantitativereal-timepolymerasechainreactionanalysisandfoundtobeconsistentwithtranscriptomesequencing.TheseresultssuggestthatMMP-relatedgenesarestronglyinvolvedintheprocessofperipheralnerveregeneration.
出版日期
2016年07月17日（中国期刊网平台首次上网日期，不代表论文的发表时间）
