摘要
CirculatingTumorCells(CTC)havethepotentialtobeusedclinicallyasadiagnostictoolandatreatmenttoolinthefieldofoncology.Asadiagnostictool,CTCmaybeusedtoindicatethepresenceofatumorbeforeitislargeenoughtocausenoticeablesymptoms.Asatreatmenttool,CTCisolatedfrompatientsmaybeusedtotesttheefficacyofchemotherapyoptionstopersonalizepatienttreatment.Onewayfortumorstospreadisthroughmetastasisviathecirculatorysystem.CTCareabletoexploitthenaturalleukocyterecruitmentprocessthatisinitiallymediatedbyrollingontransientselectinbonds.OurcapturedevicestakeadvantageofthisnaturallyoccurringrecruitmentsteptoisolateCTCfromwholebloodbyflowingsamplesthroughselectinandantibody-coatedmicrotubes.Wholebloodwasspikedwithaknownconcentrationoflabeledcancercellsandthenperfusedthroughpre-coatedmicrotubes.Microtubeswerethenrinsedtoremoveunboundcellsandthenumberoflabeledcellscapturedonthelumenwasassessed.CTCweresuccessfullycapturedfromwholebloodataclinicallyrelevantlevelontheorderof10cellspermL.Combinationtubeswithselectinandantibodycoatedsurfaceexhibitedhighercaptureratethantubescoatedwithselectinaloneorantibodyalone.Additionally,CTCcapturewasdemonstratedwiththeKG1ahematopoieticcelllineandtheDU145epithelialcellline.Thus,theinvivoprocessofselectin-mediatedCTCrecruitmenttodistantvesselwallscanbeusedinvitrototargetCTCtoatubelumen.ThebiomolecularcoatingscanalsobeusedtocaptureCTCofhematopoieticandepithelialtumororiginandisdemonstratedtosensitivitiesdowntotheorderof10CTCpermL.Inarelatedstudyaimedatreducingthebloodbornemetastaticcancerload,wehaveshownthatcellscapturedtoasurfacecanbeneutralizedbyareceptor-mediatedbiochemicalsignal.IntheproposedmethodwehaveshownthatusingacombinedselectinandTRAIL(TNFRelatedApoptosisInducingLigandorApo2L)functionalizedsurfaceweareabl
出版日期
2009年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
