摘要
Osteocytesactasmechanosensorsinbone,whichcansendmechanicalsignalsdirectlytoosteoblaststhroughgapjunctions.However,underphysiologicalconditionsthenumberofgapjunctionsislimitedbecauseofthequantityvarianceofthetwokindsofcells.Inthisstudy,thepossibilityofindirectinteractionbetweenthesetwocellswasinvestigated.Anewflowchamperwasdesignedinwhichosteocytesandosteoblastswerecoculturedintwochampersseparately.OsteocyteswereexposedtofluidflowandthentheALPactivity,osteocalcinandosteopotinofosteoblastsweredetermined.TheresultsshowedthateitherALPactivityorproductionofosteocalcinandosteopotininosteoblaststhatwerecoculturedwithshearedosteocytesincreased,whichindicatedthatosteocytescouldregulateosteoblastsindirectlythroughsomesolublefactors.
出版日期
2012年04月14日(中国期刊网平台首次上网日期,不代表论文的发表时间)